A Gram-reaction-variable, rod-shaped, motile, facultatively aerobic and endospore-forming bacterium, designated strain GSS02T, was isolated from a forest soil. Strain GSS02T was capable of reducing humic substances and Fe(III) oxides. Strain GSS02T grew optimally at 35 °C, at pH 78 and in the presence of 1 % NaCl. The predominant menaquinone was MK-7. The major cellular fatty acids were anteiso-C15 : 0 and iso-C16 : 0 and the polar lipid profile contained mainly phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylglycerol, with moderate amounts of two unknown aminophospholipids and a minor amount of one unknown lipid. The DNA G+C content was 53.4 mol%. Comparative 16S rRNA gene sequence analysis showed that strain GSS02T was related most closely to Paenibacillus terrigena JCM 21741T (98.1 % similarity). Mean DNA–DNA relatedness between strain GSS02T and P. terrigena JCM 21741T was 58.8±0.5 %. The phylogenetic, chemotaxonomic and phenotypic results clearly demonstrated that strain GSS02T belongs to the genus Paenibacillus and represents a novel species, for which the name Paenibacillus guangzhouensis sp. nov. is proposed. The type strain is GSS02T ( = KCTC 33171T = CCTCC AB 2013236T).
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Funding
This study was supported by the:
National Natural Science Foundation of China
(Award 41201227 and 41301257)
China Postdoctoral Science Foundation Grant
(Award 2013M531828)
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