1887

Abstract

In order to clarify the taxonomic position of serotypes 20, 22 and 26 of , biochemical and molecular genetic studies were performed on isolates (SUT-7, SUT-286, SUT-319, SUT-328 and SUT-380) reacted with specific antisera of serotypes 20, 22 or 26 from the saliva of healthy pigs as well as reference strains of serotypes 20, 22 and 26. Comparative gene sequencing showed high genetic relatedness among our isolates, but marked differences from the type strain NCTC 10234, i.e. 74.8–75.7 % sequence similarity. The genomic relatedness between the isolates and other strains of species of the genus , including , was calculated using the average nucleotide identity values of whole genome sequences, which indicated that serotypes 20, 22 and 26 should be removed taxonomically from and treated as a novel genomic species. Comparative sequence analysis revealed 99.0–100 % sequence similarities for the 16S rRNA genes between the reference strains of serotypes 20, 22 and 26, and our isolates. Isolate STU-286 had relatively high 16S rRNA gene sequence similarity with NCTC 10234 (98.8 %). SUT-286 could be distinguished from and other closely related species of the genus using biochemical tests. Due to its phylogenetic and phenotypic similarities to we propose naming the novel species sp. nov., with SUT-286 ( = JCM 30273 = DSM 29126) as the type strain.

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2015-02-01
2019-10-14
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