1887

Abstract

Two novel Gram-stain-negative strains (CCBAU 03422 and CCBAU 03415) isolated from root nodules of were classified phylogenetically into the genus based on the comparative analysis of 16S rRNA and genes. They showed 99.8 % rRNA gene sequence similarities to LMG 22836, and strain CCBAU 03422 showed 91.2 and 88.6 % gene sequence similarities to strains LMG 26470 and LMG 22836, respectively. Strain CCBAU 03422 contained Q-10 as its major quinone and showed a cellular fatty acid profile, carbon source utilization and other phenotypic characteristics differing from type strains of related species. DNA–DNA relatedness (lower than 48.8 %) further confirmed the differences between the novel strains and the type strains of related species. Strain CCBAU 03422 could nodulate and fix nitrogen effectively on its original host plant, . Based upon the results mentioned above, a novel species named is proposed and the type strain is CCBAU 03422 ( = A-6-3 = LMG 27899 = HAMBI 3508).

Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 31270052)
  • Chinese Universities Scientific Fund (Award 2014QT003)
  • Instituto Politecnico Nacional, Mexico (Award 2014124 and SIP 20130828)
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2015-02-01
2024-03-28
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