1887

Abstract

Two novel Gram-stain-negative strains (CCBAU 03422 and CCBAU 03415) isolated from root nodules of were classified phylogenetically into the genus based on the comparative analysis of 16S rRNA and genes. They showed 99.8 % rRNA gene sequence similarities to LMG 22836, and strain CCBAU 03422 showed 91.2 and 88.6 % gene sequence similarities to strains LMG 26470 and LMG 22836, respectively. Strain CCBAU 03422 contained Q-10 as its major quinone and showed a cellular fatty acid profile, carbon source utilization and other phenotypic characteristics differing from type strains of related species. DNA–DNA relatedness (lower than 48.8 %) further confirmed the differences between the novel strains and the type strains of related species. Strain CCBAU 03422 could nodulate and fix nitrogen effectively on its original host plant, . Based upon the results mentioned above, a novel species named is proposed and the type strain is CCBAU 03422 ( = A-6-3 = LMG 27899 = HAMBI 3508).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.067017-0
2015-02-01
2019-12-09
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/2/399.html?itemId=/content/journal/ijsem/10.1099/ijs.0.067017-0&mimeType=html&fmt=ahah

References

  1. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J.. ( 1990;). Basic local alignment search tool. . J Mol Biol 215:, 403–410. [CrossRef][PubMed]
    [Google Scholar]
  2. Bochner B. R., Gadzinski P., Panomitros E.. ( 2001;). Phenotype microarrays for high-throughput phenotypic testing and assay of gene function. . Genome Res 11:, 1246–1255. [CrossRef][PubMed]
    [Google Scholar]
  3. Chun J., Goodfellow M.. ( 1995;). A phylogenetic analysis of the genus Nocardia with 16S rRNA gene sequences. . Int J Syst Bacteriol 45:, 240–245. [CrossRef][PubMed]
    [Google Scholar]
  4. Ezaki T., Hashimoto Y., Yabuuchi E.. ( 1989;). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39:, 224–229. [CrossRef]
    [Google Scholar]
  5. Flores-Félix J.-D., Carro L., Velázquez E., Valverde Á., Cerda-Castillo E., García-Fraile P., Rivas R.. ( 2013;). Phyllobacterium endophyticum sp. nov., isolated from nodules of Phaseolus vulgaris. . Int J Syst Evol Microbiol 63:, 821–826. [CrossRef][PubMed]
    [Google Scholar]
  6. Gao J. L., Sun J. G., Li Y., Wang E. T., Chen W. X.. ( 1994;). Numerical taxonomy and DNA relatedness of tropical rhizobia isolated from Hainan Province, China. . Int J Syst Bacteriol 44:, 151–158. [CrossRef]
    [Google Scholar]
  7. Gao J. L., Turner S. L., Kan F. L., Wang E. T., Tan Z. Y., Qiu Y. H., Gu J., Terefework Z., Young J. P. W.. & other authors ( 2004;). Mesorhizobium septentrionale sp. nov. and Mesorhizobium temperatum sp. nov., isolated from Astragalus adsurgens growing in the northern regions of China. . Int J Syst Evol Microbiol 54:, 2003–2012. [CrossRef][PubMed]
    [Google Scholar]
  8. Gaunt M. W., Turner S. L., Rigottier-Gois L., Lloyd-Macgilp S. A., Young J. P.. ( 2001;). Phylogenies of atpD and recA support the small subunit rRNA-based classification of rhizobia. . Int J Syst Evol Microbiol 51:, 2037–2048. [CrossRef][PubMed]
    [Google Scholar]
  9. Jordan D. C.. ( 1984;). Family III. Rhizobiaceae. . In Bergey’s Manual of Systematic Bacteriology, vol. 1, pp. 234–242. Edited by Krieg N. R., Holt J. G... Baltimore, MD:: Williams and Wilkins;.
    [Google Scholar]
  10. Jurado V., Laiz L., Gonzalez J. M., Hernandez-Marine M., Valens M., Sáiz-Jimenez C.. ( 2005;). Phyllobacterium catacumbae sp. nov., a member of the order ‘Rhizobiales’ isolated from Roman catacombs. . Int J Syst Evol Microbiol 55:, 1487–1490. [CrossRef][PubMed]
    [Google Scholar]
  11. Knösel D. H.. ( 1962;). Prüfung von Bakterien auf Fähigkeit zür Sternbildung. . Zentralbl Bakteriol Parasitenkd Infektionskr Hyg 116:, 79–100 (in German).
    [Google Scholar]
  12. Laguerre G., Nour S. M., Macheret V., Sanjuan J., Drouin P., Amarger N.. ( 2001;). Classification of rhizobia based on nodC and nifH gene analysis reveals a close phylogenetic relationship among Phaseolus vulgaris symbionts. . Microbiology 147:, 981–993.[PubMed]
    [Google Scholar]
  13. Lee J.-S., Shin Y. K., Yoon J.-H., Takeuchi M., Pyun Y.-R., Park Y.-H.. ( 2001;). Sphingomonas aquatilis sp. nov., Sphingomonas koreensis sp. nov., and Sphingomonas taejonensis sp. nov., yellow-pigmented bacteria isolated from natural mineral water. . Int J Syst Evol Microbiol 51:, 1491–1498.[PubMed]
    [Google Scholar]
  14. Li M., Li Y., Chen W. F., Sui X. H., Li Y. Jr, Li Y., Wang E. T., Chen W. X.. ( 2012;). Genetic diversity, community structure and distribution of rhizobia in the root nodules of Caragana spp. from arid and semi-arid alkaline deserts, in the north of China. . Syst Appl Microbiol 35:, 239–245. [CrossRef][PubMed]
    [Google Scholar]
  15. Mandel M., Marmur J.. ( 1968;). Use of ultraviolet absorbance-temperature profile for determining the guanine plus cytosine content of DNA. . Methods Enzymol 12:, 195–206. [CrossRef]
    [Google Scholar]
  16. Mantelin S., Saux M. F., Zakhia F., Béna G., Bonneau S., Jeder H., de Lajudie P., Cleyet-Marel J.-C.. ( 2006;). Emended description of the genus Phyllobacterium and description of four novel species associated with plant roots: Phyllobacterium bourgognense sp. nov., Phyllobacterium ifriqiyense sp. nov., Phyllobacterium leguminum sp. nov. and Phyllobacterium brassicacearum sp. nov.. Int J Syst Evol Microbiol 56:, 827–839. [CrossRef][PubMed]
    [Google Scholar]
  17. Martinez-Romero E., Rosenblueth M.. ( 1990;). Increased bean (Phaseolus vulgaris L.) nodulation competitiveness of genetically modified Rhizobium strains. . Appl Environ Microbiol 56:, 2384–2388.[PubMed]
    [Google Scholar]
  18. Mergaert J., Swings J.. ( 2005;). Family IV. Phyllobacteriaceae fam. nov.. In Bergey’s Manual of Systematic Bacteriology, , 2nd edn., vol. 2, pp. 393–396. Edited by Brenner D. J., Krieg N. R., Staley J. T... New York:: Springer;.
    [Google Scholar]
  19. Mergaert J., Cnockaert M. C., Swings J.. ( 2002;). Phyllobacterium myrsinacearum (subjective synonym Phyllobacterium rubiacearum) emend.. Int J Syst Evol Microbiol 52:, 1821–1823. [CrossRef][PubMed]
    [Google Scholar]
  20. Rivas R., García-Fraile P., Mateos P. F., Martínez-Molina E., Velázquez E.. ( 2007;). Characterization of xylanolytic bacteria present in the bract phyllosphere of the date palm Phoenix dactylifera. . Lett Appl Microbiol 44:, 181–187. [CrossRef][PubMed]
    [Google Scholar]
  21. Rogers J. S., Swofford D. L.. ( 1998;). A fast method for approximating maximum likelihoods of phylogenetic trees from nucleotide sequences. . Syst Biol 47:, 77–89. [CrossRef][PubMed]
    [Google Scholar]
  22. Sánchez M., Ramírez-Bahena M.-H., Peix A., Lorite M. J., Sanjuán J., Velázquez E., Monza J.. ( 2014;). Phyllobacterium loti sp. nov. isolated from nodules of Lotus corniculatus. . Int J Syst Evol Microbiol 64:, 781–786. [CrossRef][PubMed]
    [Google Scholar]
  23. Sasser M.. ( 1990;). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101, Newark, DE:: MIDI Inc;.
    [Google Scholar]
  24. Smibert R. M., Krieg N. R.. ( 1994;). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R... Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  25. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S.. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739. [CrossRef][PubMed]
    [Google Scholar]
  26. Terefework Z., Kaijalainen S., Lindström K.. ( 2001;). AFLP fingerprinting as a tool to study the genetic diversity of Rhizobium galegae isolated from Galega orientalis and Galega officinalis. . J Biotechnol 91:, 169–180. [CrossRef][PubMed]
    [Google Scholar]
  27. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G.. ( 1997;). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25:, 4876–4882. [CrossRef][PubMed]
    [Google Scholar]
  28. Valverde A., Velázquez E., Fernández-Santos F., Vizcaíno N., Rivas R., Mateos P. F., Martínez-Molina E., Igual J. M., Willems A.. ( 2005;). Phyllobacterium trifolii sp. nov., nodulating Trifolium and Lupinus in Spanish soils. . Int J Syst Evol Microbiol 55:, 1985–1989. [CrossRef][PubMed]
    [Google Scholar]
  29. Vincent J. M.. ( 1970;). A Manual for the Practical Study of the Root-Nodule Bacteria, vol. 15. Oxford:: Blackwell Scientific;.
    [Google Scholar]
  30. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E.. & other authors ( 1987;). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37:, 463–464. [CrossRef]
    [Google Scholar]
  31. Willems A., Doignon-Bourcier F., Goris J., Coopman R., de Lajudie P., De Vos P., Gillis M.. ( 2001;). DNA-DNA hybridization study of Bradyrhizobium strains. . Int J Syst Evol Microbiol 51:, 1315–1322.[PubMed]
    [Google Scholar]
  32. Zhao C. T., Wang E. T., Chen W. F., Chen W. X.. ( 2008;). Diverse genomic species and evidences of symbiotic gene lateral transfer detected among the rhizobia associated with Astragalus species grown in the temperate regions of China. . FEMS Microbiol Lett 286:, 263–273. [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.067017-0
Loading
/content/journal/ijsem/10.1099/ijs.0.067017-0
Loading

Data & Media loading...

Supplements

Supplementary Data



PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error