1887

Abstract

A novel bacterial strain designated CB4 was isolated from soil from the Hallasan, Jeju, Korea. Strain CB4 was found to be strictly aerobic, Gram-stain-positive, rod-shaped, motile and formed creamy greyish colonies on nutrient agar. The major fatty acids were identified as iso-C and iso-C, and the predominant isoprenoid quinone as MK-7. The cell-wall peptidoglycan contained glycine and alanine as the diagnostic amino acids and phosphatidyl--methylethanolamine, phosphatidylethanolamine, diphosphatidylglycerol and an unidentified aminophospholipid as the polar lipids. The genomic DNA G+C content of strain CB4 was 46.5 mol%. Phylogenetic analysis, based on 16S rRNA gene sequence similarities, showed that strain CB4 forms a deep branch within the genus , sharing the highest level of sequence homology with DSM 5562 (96.5 %). On the basis of the phenotypic, chemotaxonomic and phylogenetic characteristics, strain CB4 is considered to represent a novel species within the genus , for which the name sp. nov. is proposed. The type strain is CB4 ( = KCTC 33505 = CECT 8566). An emended description of the genus is also proposed.

Funding
This study was supported by the:
  • Ministry of Science, ICT and Future Planning
  • KRIBB Research Initiative Program
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.066779-0
2014-11-01
2024-04-23
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/64/11/3792.html?itemId=/content/journal/ijsem/10.1099/ijs.0.066779-0&mimeType=html&fmt=ahah

References

  1. Allan R. N., Lebbe L., Heyrman J., De Vos P., Buchanan C. J., Logan N. A. ( 2005 ). Brevibacillus levickii sp. nov. and Aneurinibacillus terranovensis sp. nov., two novel thermoacidophiles isolated from geothermal soils of northern Victoria Land, Antarctica. . Int J Syst Evol Microbiol 55, 10391050. [View Article] [PubMed]
    [Google Scholar]
  2. Aoyama S. ( 1952 ). Studies on the thiamin-decomposing bacterium. I. Bacteriological researches of a new thiamin decomposing bacillus Bacillus aneurinolyticus Kimura et Aoyama. . Acta Sch Med Univ Kioto 30, 127132.[PubMed]
    [Google Scholar]
  3. Bower V. E., Bates R. G. ( 1955 ). pH Values of the Clark and Lubs Buffer Solutions at 25 °C. . J Res Natl Bur Stand 55, 197200. [View Article]
    [Google Scholar]
  4. Felsenstein J. ( 1981 ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17, 368376. [View Article] [PubMed]
    [Google Scholar]
  5. Felsenstein J. ( 1985 ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39, 783791. [View Article]
    [Google Scholar]
  6. Felsenstein J. ( 2009 ). phylip (phylogeny inference package) version 3.69. Distributed by the author. . Department of Genome Sciences, University of Washington;, Seattle, USA:.
    [Google Scholar]
  7. Fitch W. M. ( 1971 ). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20, 406416. [View Article]
    [Google Scholar]
  8. Goto K., Fujita R., Kato Y., Asahara M., Yokota A. ( 2004 ). Reclassification of Brevibacillus brevis strains NCIMB 13288 and DSM 6472 ( = NRRL NRS-887) as Aneurinibacillus danicus sp. nov. and Brevibacillus limnophilus sp. nov.. Int J Syst Evol Microbiol 54, 419427. [View Article] [PubMed]
    [Google Scholar]
  9. Hall T. A. ( 1999 ). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. . Nucleic Acids Symp Ser 41, 9598.
    [Google Scholar]
  10. Heyndrickx M., Lebbe L., Vancanneyt M., Kersters K., De Vos P., Logan N. A., Forsyth G., Nazli S., Ali N., Berkeley R. C. W. ( 1997 ). A polyphasic reassessment of the genus Aneurinibacillus, reclassification of Bacillus thermoaerophilus (Meier-Stauffer et al., 1996) as Aneurinibacillus thermoaerophilus comb. nov., and emended descriptions of A. aneurinilyticus corrig., A. migulanus, and A. thermoaerophilus . . Int J Syst Bacteriol 47, 808817. [View Article]
    [Google Scholar]
  11. Jukes T. H., Cantor C. R. ( 1969 ). Evolution of protein molecules. . In Mammalian Protein Metabolism, vol. 3, pp. 21132. Edited by Munro H. N. . New York:: Academic Press;. [View Article]
    [Google Scholar]
  12. Lányí B. ( 1987 ). Classical and rapid identification methods for medically important bacteria. . Methods Microbiol 19, 167. [View Article]
    [Google Scholar]
  13. Lee K. C., Kim K. K., Eom M. K., Kim M. J., Lee J.-S. ( 2011 ). Fontibacillus panacisegetis sp. nov., isolated from soil of a ginseng field. . Int J Syst Evol Microbiol 61, 369374. [View Article] [PubMed]
    [Google Scholar]
  14. Meier-Stauffer K., Busse H.-J., Rainey F. A., Burghardt J., Scheberl A., Hollaus F., Kuen B., Makristathis A., Sleytr U. B., Messner P. ( 1996 ). Description of Bacillus thermoaerophilus sp. nov., to include sugar beet isolates and Bacillus brevis ATCC 12990. . Int J Syst Bacteriol 46, 532541. [View Article]
    [Google Scholar]
  15. Minnikin D. E., Patel P. V., Alshamaony L., Goodfellow M. ( 1977 ). Polar lipid composition in the classification of Nocardia and related bacteria. . Int J Syst Bacteriol 27, 104117. [View Article]
    [Google Scholar]
  16. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  17. Sasser M. ( 1990 ). Identification of bacteria by gas chromatography of cellular fatty acids. MIDI Technical Note 101. Newark, DE:: MIDI Inc;.
    [Google Scholar]
  18. Schleifer K. H., Kandler O. ( 1972 ). Peptidoglycan types of bacterial cell walls and their taxonomic implications. . Bacteriol Rev 36, 407477.[PubMed]
    [Google Scholar]
  19. Shida O., Takagi H., Kadowaki K., Yano H., Abe M., Udaka S., Komagata K. ( 1994 ). Bacillus aneurinolyticus sp. nov., nom. rev.. Int J Syst Bacteriol 44, 143150. [View Article]
    [Google Scholar]
  20. Shida O., Takagi H., Kadowaki K., Komagata K. ( 1996 ). Proposal for two new genera, Brevibacillus gen. nov. and Aneurinibacillus gen. nov.. Int J Syst Bacteriol 46, 939946. [View Article] [PubMed]
    [Google Scholar]
  21. Shin Y. K., Lee J.-S., Chun C. O., Kim H.-J., Park Y.-H. ( 1996 ). Isoprenoid quinone profiles of Leclercia adecarboxylata KCTC 1036T . . J Microbiol Biotechnol 6, 6869.
    [Google Scholar]
  22. Skerman V. B. D. ( 1967 ). A Guide to the Identfication of the Genera of Bacteria, , 2nd edn.. Baltimore:: Williams & Wilkins;.
    [Google Scholar]
  23. Stackebrandt E., Goebel B. M. ( 1994 ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol 44, 846849. [View Article]
    [Google Scholar]
  24. Takagi H., Shida O., Kadowaki K., Komagata K., Udaka S. ( 1993 ). Characterization of Bacillus brevis with descriptions of Bacillus migulanus sp. nov., Bacillus choshinensis sp. nov., Bacillus parabrevis sp. nov., and Bacillus galactophilus sp. nov.. Int J Syst Bacteriol 43, 221231. [View Article] [PubMed]
    [Google Scholar]
  25. Tamaoka J., Komagata K. ( 1984 ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. . FEMS Microbiol Lett 25, 125128. [View Article]
    [Google Scholar]
  26. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. ( 1997 ). The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25, 48764882. [View Article] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.066779-0
Loading
/content/journal/ijsem/10.1099/ijs.0.066779-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error