1887

Abstract

A Gram-stain-negative bacterium, designated SP-35, was isolated from compost and was subjected to a taxonomic study. This isolate was short-rod-shaped and non-spore-forming. Phylogenetic analysis based on 16S rRNA sequence comparison indicated the isolate was related to the genus . 16S rRNA gene sequence analysis showed that its closest neighbours were the type strains Dant 3-8 (96.8 % similarity), DSM 50244 (96.5 %), CY01 (95.9 %) and YY287 (95.6 %). Using phylogenetic analysis, DNA–DNA hybridization, fatty acid composition data and a range of physiological and biochemical characteristics we could clearly distinguish strain SP-35 from type strains of the genus . The genomic DNA G+C content of strain SP-35 was 63.1 mol%. The predominant cellular fatty acids were C, C cyclo, summed feature 3 (Cω6 and/or Cω7) and summed feature 8 (Cω6 and/or Cω7). The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidlyglycerol. Differences in phenotypic and phylogenetic characteristics support the classification of strain SP-35 as a representative of a novel species in the genus , for which the name sp. nov. is proposed. The type strain is SP-35 ( = DSM 26136 = JCM 18194).

Funding
This study was supported by the:
  • , Natural Science Foundation of Jiangsu Province, China , (Award BK2012695)
  • , Technology Innovation Fund for Small and Medium-sized Enterprises of Jiangsu Province, China , (Award BC2013209)
  • , Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry, China
  • , Priority Academic Program Development of Jiangsu Higher Education Institutions
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2014-12-01
2021-03-07
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