1887

Abstract

A novel Fe(III)-reducing bacterium, designated GSS01, was isolated from a forest soil sample using a liquid medium containing acetate and ferrihydrite as electron donor and electron acceptor, respectively. Cells of strain GSS01 were strictly anaerobic, Gram-stain-negative, motile, non-spore-forming and slightly curved rod-shaped. Growth occurred at 16–40 °C and optimally at 30 °C. The DNA G+C content was 60.9 mol%. The major respiratory quinone was MK-8. The major fatty acids were C, C and Cω7/Cω6. Strain GSS01 was able to grow with ferrihydrite, Fe(III) citrate, Mn(IV), sulfur, nitrate or anthraquinone-2,6-disulfonate, but not with fumarate, as sole electron acceptor when acetate was the sole electron donor. The isolate was able to utilize acetate, ethanol, glucose, lactate, butyrate, pyruvate, benzoate, benzaldehyde, -cresol and phenol but not toluene, -cresol, propionate, malate or succinate as sole electron donor when ferrihydrite was the sole electron acceptor. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain GSS01 was most closely related to PCA (98.3 % sequence similarity) and exhibited low similarities (94.9–91.8 %) to the type strains of other species of the genus . The DNA–DNA relatedness between strain GSS01 and PCA was 41.4±1.1 %. On the basis of phylogenetic analysis, phenotypic characterization and physiological tests, strain GSS01 is believed to represent a novel species of the genus , and the name sp. nov. is proposed. The type strain is GSS01 ( = KCTC 4545 = MCCC 1K00269).

Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 41301257 and 41222006)
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2014-11-01
2021-07-29
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References

  1. Baker G. C., Smith J. J., Cowan D. A. ( 2003 ). Review and re-analysis of domain-specific 16S primers. . J Microbiol Methods 55, 541555. [View Article] [PubMed]
    [Google Scholar]
  2. Canfield D. E., Kristensen E., Thamdrup B. ( 2005 ). The iron and manganese cycles. . Adv Mar Biol 48, 269312. [View Article]
    [Google Scholar]
  3. Coates J. D., Phillips E. J., Lonergan D. J., Jenter H., Lovley D. R. ( 1996 ). Isolation of Geobacter species from diverse sedimentary environments. . Appl Environ Microbiol 62, 15311536.[PubMed]
    [Google Scholar]
  4. Coates J. D., Bhupathiraju V. K., Achenbach L. A., Mclnerney M. J., Lovley D. R. ( 2001 ). Geobacter hydrogenophilus, Geobacter chapellei and Geobacter grbiciae, three new, strictly anaerobic, dissimilatory Fe(III)-reducers. . Int J Syst Evol Microbiol 51, 581588.[PubMed]
    [Google Scholar]
  5. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E. ( 1977 ). Distribution of menaquinones in actinomycetes and corynebacteria. . J Gen Microbiol 100, 221230. [View Article] [PubMed]
    [Google Scholar]
  6. Ding P., Shen C., Wang N., Yi W., Liu K., Ding X., Fu D. ( 2009 ). Carbon isotopic composition and its implications on paleoclimate of the underground ancient forest ecosystem in Sihui, Guangdong. . Science in China Series D: Earth Sciences 52, 638646. [View Article]
    [Google Scholar]
  7. Ezaki T., Hashimoto Y., Yabuuchi E. ( 1989 ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39, 224229. [View Article]
    [Google Scholar]
  8. Felsenstein J. ( 1985 ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39, 783791. [View Article]
    [Google Scholar]
  9. Fredrickson J. K., Gorby Y. A. ( 1996 ). Environmental processes mediated by iron-reducing bacteria. . Curr Opin Biotechnol 7, 287294. [View Article] [PubMed]
    [Google Scholar]
  10. Groth I., Schumann P., Rainey F. A., Martin K., Schuetze B., Augsten K. ( 1997 ). Demetria terragena gen. nov., sp. nov., a new genus of actinomycetes isolated from compost soil. . Int J Syst Bacteriol 47, 11291133. [View Article] [PubMed]
    [Google Scholar]
  11. Hedrick D. B., Peacock A. D., Lovley D. R., Woodard T. L., Nevin K. P., Long P. E., White D. C. ( 2009 ). Polar lipid fatty acids, LPS-hydroxy fatty acids, and respiratory quinones of three Geobacter strains, and variation with electron acceptor. . J Ind Microbiol Biotechnol 36, 205209. [View Article] [PubMed]
    [Google Scholar]
  12. Kim O.-S., Cho Y.-J., Lee K., Yoon S.-H., Kim M., Na H., Park S.-C., Jeon Y. S., Lee J.-H. & other authors ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716721. [View Article] [PubMed]
    [Google Scholar]
  13. Kunapuli U., Jahn M. K., Lueders T., Geyer R., Heipieper H. J., Meckenstock R. U. ( 2010 ). Desulfitobacterium aromaticivorans sp. nov. and Geobacter toluenoxydans sp. nov., iron-reducing bacteria capable of anaerobic degradation of monoaromatic hydrocarbons. . Int J Syst Evol Microbiol 60, 686695. [View Article] [PubMed]
    [Google Scholar]
  14. Li X. M., Zhou S. G., Li F. B., Wu C. Y., Zhuang L., Xu W., Liu L. ( 2009 ). Fe(III) oxide reduction and carbon tetrachloride dechlorination by a newly isolated Klebsiella pneumoniae strain L17. . J Appl Microbiol 106, 130139. [View Article] [PubMed]
    [Google Scholar]
  15. Lovley D. R., Giovannoni S. J., White D. C., Champine J. E., Phillips E. J., Gorby Y. A., Goodwin S. ( 1993 ). Geobacter metallireducens gen. nov. sp. nov., a microorganism capable of coupling the complete oxidation of organic compounds to the reduction of iron and other metals. . Arch Microbiol 159, 336344. [View Article] [PubMed]
    [Google Scholar]
  16. Lovley D. R., Holmes D. E., Nevin K. P. ( 2004 ). Dissimilatory Fe(III) and Mn(IV) reduction. . Adv Microb Physiol 49, 219286. [View Article] [PubMed]
    [Google Scholar]
  17. Mesbah M., Premachandran U., Whitman W. B. ( 1989 ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39, 159167. [View Article]
    [Google Scholar]
  18. Prakash O., Gihring T. M., Dalton D. D., Chin K. J., Green S. J., Akob D. M., Wanger G., Kostka J. E. ( 2010 ). Geobacter daltonii sp. nov., an Fe(III)- and uranium(VI)-reducing bacterium isolated from a shallow subsurface exposed to mixed heavy metal and hydrocarbon contamination. . Int J Syst Evol Microbiol 60, 546553. [View Article] [PubMed]
    [Google Scholar]
  19. Roden E. E., Wetzel R. G. ( 1996 ). Organic carbon oxidation and suppression of methane production by microbial Fe(III) oxide reduction in vegetated and unvegetated freshwater wetland sediments. . Limnol Oceanogr 41, 17331748. [View Article]
    [Google Scholar]
  20. Shelobolina E. S., Nevin K. P., Blakeney-Hayward J. D., Johnsen C. V., Plaia T. W., Krader P., Woodard T., Holmes D. E., Vanpraagh C. G., Lovley D. R. ( 2007 ). Geobacter pickeringii sp. nov., Geobacter argillaceus sp. nov. and Pelosinus fermentans gen. nov., sp. nov., isolated from subsurface kaolin lenses. . Int J Syst Evol Microbiol 57, 126135. [View Article] [PubMed]
    [Google Scholar]
  21. Shelobolina E. S., Vrionis H. A., Findlay R. H., Lovley D. R. ( 2008 ). Geobacter uraniireducens sp. nov., isolated from subsurface sediment undergoing uranium bioremediation. . Int J Syst Evol Microbiol 58, 10751078. [View Article] [PubMed]
    [Google Scholar]
  22. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 27312739. [View Article] [PubMed]
    [Google Scholar]
  23. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. ( 1997 ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25, 48764882. [View Article] [PubMed]
    [Google Scholar]
  24. Viulu S., Nakamura K., Kojima A., Yoshiyasu Y., Saitou S., Takamizawa K. ( 2013a ). Geobacter sulfurreducens subsp. ethanolicus, subsp. nov., an ethanol-utilizing dissimilatory Fe(III)-reducing bacterium from a lotus field. . J Gen Appl Microbiol 59, 325334. [View Article] [PubMed]
    [Google Scholar]
  25. Viulu S., Nakamura K., Okada Y., Saitou S., Takamizawa K. ( 2013b ). Geobacter luticola sp. nov., an Fe(III)-reducing bacterium isolated from lotus field mud. . Int J Syst Evol Microbiol 63, 442448. [View Article] [PubMed]
    [Google Scholar]
  26. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. et al. ( 1987 ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37, 463464. [View Article]
    [Google Scholar]
  27. Wu C. Y., Zhuang L., Zhou S. G., Li F. B., Li X. M. ( 2010 ). Fe(III)-enhanced anaerobic transformation of 2,4-dichlorophenoxyacetic acid by an iron-reducing bacterium Comamonas koreensis CY01. . FEMS Microbiol Ecol 71, 106113. [View Article] [PubMed]
    [Google Scholar]
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