1887

Abstract

A Gram-stain-negative, coccoid- or oval-shaped, gliding bacterial strain, designated HDW-31, belonging to the class , was isolated from seawater of the Yellow Sea, Korea, and was subjected to a taxonomic study using a polyphasic approach. Strain HDW-31 grew optimally at pH 7.0–8.0, at 30 °C and in the presence of 2–3 % (w/v) NaCl. Neighbour-joining, maximum-likelihood and maximum-parsimony phylogenetic trees based on 16S rRNA gene sequences showed that strain HDW-31 fell within the clade comprising the genus , clustering with the type strains of and , with which strain HDW-31 exhibited 97.0 and 96.0 % sequence similarity values, respectively. Sequence similarities to the type strains of the other recognized species of the genus were 93.5–96.0 %. The DNA G+C content was 57.9 mol% and mean DNA–DNA relatedness between strain HDW-31 and the type strain of was 5.3 %. Strain HDW-31 contained Q-10 as the predominant ubiquinone and summed feature 8 (Cω7 and/or Cω6), summed feature 3 (Cω6 and/or Cω7) and C as the major fatty acids. The major polar lipids were phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, a sphingoglycolipid, two unidentified glycolipids and an unidentified lipid. Differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, demonstrated that strain HDW-31 is distinguishable from recognized species of the genus . On the basis of the data presented, strain HDW-31 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is HDW-31 ( = KCTC 42006 = CECT 8527).

Funding
This study was supported by the:
  • National Institute of Biological Resources (NIBR)
  • Ministry of Environment (MOE)
  • Program for Collection, Management and Utilization of Biological Resources
  • BK 21 program
  • Ministry of Science, ICT & Future Planning (MSIP) of the Republic of Korea
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2014-12-01
2021-10-22
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