1887

Abstract

A group of strains representing species of the genus isolated from patients with leptospirosis in Mayotte (Indian Ocean), were previously found to be considerably divergent from other known species of the genus . This was inferred from sequence analysis of (16S rRNA) and other genetic loci and suggests that they belong to a novel species. Two strains from each serogroup currently identified within this novel species were studied. Spirochaete, aerobic, motile, helix-shaped strains grew well at 30–37 °C, but not at 13 °C or in the presence of 8-azaguanine. Draft genomes of the strains were also analysed to study the DNA relatedness with other species of the genus . The new isolates formed a distinct clade, which was most closely related to , in multilocus sequence analysis using concatenated sequences of the genes , , , , and . Analysis of average nucleotide identity and genome-to-genome distances, which have recently been proposed as reliable substitutes for classical DNA–DNA hybridization, further confirmed that these isolates should be classified as representatives of a novel species. The G+C content of the genomic DNA was 39.5 mol%. These isolates are considered to represent a novel species, for which the name sp. nov. is proposed, with 200901116 ( = CIP 110703 = DSM 28999) as the type strain.

Funding
This study was supported by the:
  • , National Institute of Allergy and Infectious Diseases, National Institutes of Health, Department of Health and Human Services , (Award HHSN272200900007C)
  • , Institut Pasteur
  • , French Ministry of Health (InVS)
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2014-12-01
2020-09-29
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