1887

Abstract

A Gram-stain-positive, rod-shaped, endospore-forming bacterium, strain D45, was isolated from soil in Nanjing, China. The organism grew optimally at 30 °C, pH 7.0 and with 0 % NaCl (w/v). The 16S rRNA gene sequence of the isolate showed similarities lower than 97 % with respect to species of the genus . The predominant respiratory quinone was MK-7, with MK-6 present as a minor component; anteiso-C and iso-C were the major fatty acids. The polar lipids of strain D45 were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, two aminophospholipids, four phospholipids, two glycolipids, one aminolipid and two lipids. The DNA G+C content was 59.5 mol%. DNA–DNA hybridization of the isolate with two reference strains showed relatedness values of 33.4 % with DSM 18997 and 25.8 % with DSM 17683. The phylogenetic, chemotaxonomic and phenotypic data supported the classification of strain D45 as a representative of a novel species of the genus , for which the name sp. nov. is proposed. The type strain is D45 ( = CCTCC AB 2014067 = DSM 28246).

Funding
This study was supported by the:
  • National Nature Science Foundation of China (Award 41201251)
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2014-09-01
2021-10-26
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