1887

Abstract

A Gram-stain-positive, rod-shaped, endospore-forming bacterium, strain D45, was isolated from soil in Nanjing, China. The organism grew optimally at 30 °C, pH 7.0 and with 0 % NaCl (w/v). The 16S rRNA gene sequence of the isolate showed similarities lower than 97 % with respect to species of the genus . The predominant respiratory quinone was MK-7, with MK-6 present as a minor component; anteiso-C and iso-C were the major fatty acids. The polar lipids of strain D45 were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, two aminophospholipids, four phospholipids, two glycolipids, one aminolipid and two lipids. The DNA G+C content was 59.5 mol%. DNA–DNA hybridization of the isolate with two reference strains showed relatedness values of 33.4 % with DSM 18997 and 25.8 % with DSM 17683. The phylogenetic, chemotaxonomic and phenotypic data supported the classification of strain D45 as a representative of a novel species of the genus , for which the name sp. nov. is proposed. The type strain is D45 ( = CCTCC AB 2014067 = DSM 28246).

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2014-09-01
2019-11-22
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References

  1. Cai F. , Wang Y. , Qi H. , Dai J. , Yu B. , An H. , Rahman E. , Fang C. . ( 2010; ). Cohnella luojiensis sp. nov., isolated from soil of a Euphrates poplar forest. . Int J Syst Evol Microbiol 60:, 1605–1608. [CrossRef] [PubMed]
    [Google Scholar]
  2. Cho E. A. , Lee J. S. , Lee K. C. , Jung H. C. , Pan J. G. , Pyun Y. R. . ( 2007; ). Cohnella laeviribosi sp. nov., isolated from a volcanic pond. . Int J Syst Evol Microbiol 57:, 2902–2907. [CrossRef] [PubMed]
    [Google Scholar]
  3. Collins M. D. . ( 1985; ). Isoprenoid quinone analysis in classification and identification. . In Chemical Methods in Bacterial Systematics, pp. 267–287. Edited by Goodfellow M. , Minnikin D. E. . . London:: Academic Press;.
    [Google Scholar]
  4. De Ley J. , Cattoir H. , Reynaerts A. . ( 1970; ). The quantitative measurement of DNA hybridization from renaturation rates. . Eur J Biochem 12:, 133–142. [CrossRef] [PubMed]
    [Google Scholar]
  5. Flores-Félix J. D. , Carro L. , Ramírez-Bahena M.-H. , Tejedor C. , Igual J. M. , Peix A. , Velázquez E. . ( 2014; ). Cohnella lupini sp. nov., an endophytic bacterium isolated from root nodules of Lupinus albus . . Int J Syst Evol Microbiol 64:, 83–87. [CrossRef] [PubMed]
    [Google Scholar]
  6. García-Fraile P. , Velázquez E. , Mateos P. F. , Martínez-Molina E. , Rivas R. . ( 2008; ). Cohnella phaseoli sp. nov., isolated from root nodules of Phaseolus coccineus in Spain, and emended description of the genus Cohnella . . Int J Syst Evol Microbiol 58:, 1855–1859. [CrossRef] [PubMed]
    [Google Scholar]
  7. Gordon R. E. , Barnett D. A. , Handerhan J. E. , Pang C. H.-N. . ( 1974; ). Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. . Int J Syst Bacteriol 24:, 54–63. [CrossRef]
    [Google Scholar]
  8. Hameed A. , Hung M.-H. , Lin S.-Y. , Hsu Y.-H. , Liu Y.-C. , Shahina M. , Lai W.-A. , Huang H.-C. , Young L.-S. , Young C.-C. . ( 2013; ). Cohnella formosensis sp. nov., a xylanolytic bacterium isolated from the rhizosphere of Medicago sativa L.. Int J Syst Evol Microbiol 63:, 2806–2812. [CrossRef] [PubMed]
    [Google Scholar]
  9. Huang Z. , Sheng X.-F. , Zhao F. , He L.-Y. , Huang J. , Wang Q. . ( 2012; ). Isoptericola nanjingensis sp. nov., a mineral-weathering bacterium. . Int J Syst Evol Microbiol 62:, 971–976. [CrossRef] [PubMed]
    [Google Scholar]
  10. Jiang F. , Dai J. , Wang Y. , Xue X. , Xu M. , Li W. , Fang C. , Peng F. . ( 2012; ). Cohnella arctica sp. nov., isolated from Arctic tundra soil. . Int J Syst Evol Microbiol 62:, 817–821. [CrossRef] [PubMed]
    [Google Scholar]
  11. Kämpfer P. , Rosselló-Mora R. , Falsen E. , Busse H.-J. , Tindall B. J. . ( 2006; ). Cohnella thermotolerans gen. nov., sp. nov., and classification of ‘Paenibacillus hongkongensis’ as Cohnella hongkongensis sp. nov.. Int J Syst Evol Microbiol 56:, 781–786. [CrossRef] [PubMed]
    [Google Scholar]
  12. Kämpfer P. , Glaeser S. P. , McInroy J. A. , Busse H.-J. . ( 2014; ). Cohnella rhizosphaerae sp. nov., isolated from the rhizosphere environment of Zea mays . . Int J Syst Evol Microbiol 64:, 1811–1816. [CrossRef] [PubMed]
    [Google Scholar]
  13. Khianngam S. , Tanasupawat S. , Akaracharanya A. , Kim K. K. , Lee K. C. , Lee J. S. . ( 2010a; ). Cohnella xylanilytica sp. nov. and Cohnella terrae sp. nov., xylanolytic bacteria from soil. . Int J Syst Evol Microbiol 60:, 2913–2917. [CrossRef] [PubMed]
    [Google Scholar]
  14. Khianngam S. , Tanasupawat S. , Akaracharanya A. , Kim K. K. , Lee K. C. , Lee J. S. . ( 2010b; ). Cohnella thailandensis sp. nov., a xylanolytic bacterium from Thai soil. . Int J Syst Evol Microbiol 60:, 2284–2287. [CrossRef] [PubMed]
    [Google Scholar]
  15. Khianngam S. , Tanasupawat S. , Akaracharanya A. , Kim K. K. , Lee K. C. , Lee J. S. . ( 2012; ). Cohnella cellulosilytica sp. nov., isolated from buffalo faeces. . Int J Syst Evol Microbiol 62:, 1921–1925. [CrossRef] [PubMed]
    [Google Scholar]
  16. Kim S. J. , Weon H. Y. , Kim Y. S. , Anandham R. , Jeon Y. A. , Hong S. B. , Kwon S.-W. . ( 2010; ). Cohnella yongneupensis sp. nov. and Cohnella ginsengisoli sp. nov., isolated from two different soils. . Int J Syst Evol Microbiol 60:, 526–530. [CrossRef] [PubMed]
    [Google Scholar]
  17. Kim S. J. , Weon H. Y. , Kim Y. S. , Kwon S.-W. . ( 2011; ). Cohnella soli sp. nov. and Cohnella suwonensis sp. nov. Isolated from soil samples in Korea. . J Microbiol 49:, 1033–1038. [CrossRef] [PubMed]
    [Google Scholar]
  18. Kim O. S. , Cho Y. J. , Lee K. , Yoon S. H. , Kim M. , Na H. , Park S. C. , Jeon Y. S. , Lee J. H. . & other authors ( 2012; ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62:, 716–721. [CrossRef] [PubMed]
    [Google Scholar]
  19. Luo X. , Wang Z. , Dai J. , Zhang L. , Fang C. . ( 2010; ). Cohnella damensis sp. nov., a motile xylanolytic bacteria isolated from a low altitude area in Tibet. . J Microbiol Biotechnol 20:, 410–414.[PubMed] [CrossRef]
    [Google Scholar]
  20. Mayilraj S. , Ruckmani A. , Kaur C. , Kaur I. , Klenk H. P. . ( 2011; ). Cohnella ferri sp. nov. a novel member of the genus Cohnella isolated from haematite ore. . Curr Microbiol 62:, 1704–1709.[CrossRef]
    [Google Scholar]
  21. Mesbah M. , Premachandran U. , Whitman W. B. . ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high performance liquid chromatography. . Int J Syst Bacteriol 39:, 159–167. [CrossRef]
    [Google Scholar]
  22. Rhuland L. E. , Work E. , Denman R. F. , Hoare D. S. . ( 1955; ). The behavior of the isomers of α,ϵ-diaminopimelic acid on paper chromatograms. . J Am Chem Soc 77:, 4844–4846. [CrossRef]
    [Google Scholar]
  23. Shen P. , Fan X. R. , Li G. W. . ( 1999; ). Experiment of Microbiology. Beijing:: Higher Education Press;.
    [Google Scholar]
  24. Shiratori H. , Tagami Y. , Beppu T. , Ueda K. . ( 2010; ). Cohnella fontinalis sp. nov., a xylanolytic bacterium isolated from fresh water. . Int J Syst Evol Microbiol 60:, 1344–1348. [CrossRef] [PubMed]
    [Google Scholar]
  25. Stackebrandt E. , Goebel B. M. . ( 1994; ). Taxonomic note: a place for DNA–DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol 44:, 846–849. [CrossRef]
    [Google Scholar]
  26. Tindall B. J. . ( 1990a; ). A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. . Syst Appl Microbiol 13:, 128–130. [CrossRef]
    [Google Scholar]
  27. Tindall B. J. . ( 1990b; ). Lipid composition of Halobacterium lacusprofundi . . FEMS Microbiol Lett 66:, 199–202. [CrossRef]
    [Google Scholar]
  28. Wang L.-Y. , Chen S.-F. , Wang L. , Zhou Y.-G. , Liu H.-C. . ( 2012; ). Cohnella plantaginis sp. nov., a novel nitrogen-fixing species isolated from plantain rhizosphere soil. . Antonie van Leeuwenhoek 102:, 83–89. [CrossRef] [PubMed]
    [Google Scholar]
  29. Wayne L. G. , Brenner D. J. , Colwell R. R. , Grimont P. A. D. , Kandler O. , Krichevsky M. I. , Moore L. H. , Moore W. E. C. , Murray R. G. E. . & other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37:, 463–464. [CrossRef]
    [Google Scholar]
  30. Yoon J. H. , Jung Y. T. . ( 2012; ). Cohnella boryungensis sp. nov., isolated from soil. . Antonie van Leeuwenhoek 101:, 769–775. [CrossRef] [PubMed]
    [Google Scholar]
  31. Yoon M. H. , Ten L. N. , Im W. T. . ( 2007; ). Cohnella panacarvi sp. nov., a xylanolytic bacterium isolated from ginseng cultivating soil. . J Microbiol Biotechnol 17:, 913–918.[PubMed]
    [Google Scholar]
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