1887

Abstract

A Gram-stain-positive, rod-shaped, endospore-forming bacterium, strain D45, was isolated from soil in Nanjing, China. The organism grew optimally at 30 °C, pH 7.0 and with 0 % NaCl (w/v). The 16S rRNA gene sequence of the isolate showed similarities lower than 97 % with respect to species of the genus . The predominant respiratory quinone was MK-7, with MK-6 present as a minor component; anteiso-C and iso-C were the major fatty acids. The polar lipids of strain D45 were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, two aminophospholipids, four phospholipids, two glycolipids, one aminolipid and two lipids. The DNA G+C content was 59.5 mol%. DNA–DNA hybridization of the isolate with two reference strains showed relatedness values of 33.4 % with DSM 18997 and 25.8 % with DSM 17683. The phylogenetic, chemotaxonomic and phenotypic data supported the classification of strain D45 as a representative of a novel species of the genus , for which the name sp. nov. is proposed. The type strain is D45 ( = CCTCC AB 2014067 = DSM 28246).

Funding
This study was supported by the:
  • National Nature Science Foundation of China (Award 41201251)
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2014-09-01
2024-12-09
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References

  1. Cai F., Wang Y., Qi H., Dai J., Yu B., An H., Rahman E., Fang C. ( 2010 ). Cohnella luojiensis sp. nov., isolated from soil of a Euphrates poplar forest. . Int J Syst Evol Microbiol 60, 16051608. [View Article] [PubMed]
    [Google Scholar]
  2. Cho E. A., Lee J. S., Lee K. C., Jung H. C., Pan J. G., Pyun Y. R. ( 2007 ). Cohnella laeviribosi sp. nov., isolated from a volcanic pond. . Int J Syst Evol Microbiol 57, 29022907. [View Article] [PubMed]
    [Google Scholar]
  3. Collins M. D. ( 1985 ). Isoprenoid quinone analysis in classification and identification. . In Chemical Methods in Bacterial Systematics, pp. 267287. Edited by Goodfellow M., Minnikin D. E. . London:: Academic Press;.
    [Google Scholar]
  4. De Ley J., Cattoir H., Reynaerts A. ( 1970 ). The quantitative measurement of DNA hybridization from renaturation rates. . Eur J Biochem 12, 133142. [View Article] [PubMed]
    [Google Scholar]
  5. Flores-Félix J. D., Carro L., Ramírez-Bahena M.-H., Tejedor C., Igual J. M., Peix A., Velázquez E. ( 2014 ). Cohnella lupini sp. nov., an endophytic bacterium isolated from root nodules of Lupinus albus . . Int J Syst Evol Microbiol 64, 8387. [View Article] [PubMed]
    [Google Scholar]
  6. García-Fraile P., Velázquez E., Mateos P. F., Martínez-Molina E., Rivas R. ( 2008 ). Cohnella phaseoli sp. nov., isolated from root nodules of Phaseolus coccineus in Spain, and emended description of the genus Cohnella . . Int J Syst Evol Microbiol 58, 18551859. [View Article] [PubMed]
    [Google Scholar]
  7. Gordon R. E., Barnett D. A., Handerhan J. E., Pang C. H.-N. ( 1974 ). Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. . Int J Syst Bacteriol 24, 5463. [View Article]
    [Google Scholar]
  8. Hameed A., Hung M.-H., Lin S.-Y., Hsu Y.-H., Liu Y.-C., Shahina M., Lai W.-A., Huang H.-C., Young L.-S., Young C.-C. ( 2013 ). Cohnella formosensis sp. nov., a xylanolytic bacterium isolated from the rhizosphere of Medicago sativa L.. Int J Syst Evol Microbiol 63, 28062812. [View Article] [PubMed]
    [Google Scholar]
  9. Huang Z., Sheng X.-F., Zhao F., He L.-Y., Huang J., Wang Q. ( 2012 ). Isoptericola nanjingensis sp. nov., a mineral-weathering bacterium. . Int J Syst Evol Microbiol 62, 971976. [View Article] [PubMed]
    [Google Scholar]
  10. Jiang F., Dai J., Wang Y., Xue X., Xu M., Li W., Fang C., Peng F. ( 2012 ). Cohnella arctica sp. nov., isolated from Arctic tundra soil. . Int J Syst Evol Microbiol 62, 817821. [View Article] [PubMed]
    [Google Scholar]
  11. Kämpfer P., Rosselló-Mora R., Falsen E., Busse H.-J., Tindall B. J. ( 2006 ). Cohnella thermotolerans gen. nov., sp. nov., and classification of ‘Paenibacillus hongkongensis’ as Cohnella hongkongensis sp. nov.. Int J Syst Evol Microbiol 56, 781786. [View Article] [PubMed]
    [Google Scholar]
  12. Kämpfer P., Glaeser S. P., McInroy J. A., Busse H.-J. ( 2014 ). Cohnella rhizosphaerae sp. nov., isolated from the rhizosphere environment of Zea mays . . Int J Syst Evol Microbiol 64, 18111816. [View Article] [PubMed]
    [Google Scholar]
  13. Khianngam S., Tanasupawat S., Akaracharanya A., Kim K. K., Lee K. C., Lee J. S. ( 2010a ). Cohnella xylanilytica sp. nov. and Cohnella terrae sp. nov., xylanolytic bacteria from soil. . Int J Syst Evol Microbiol 60, 29132917. [View Article] [PubMed]
    [Google Scholar]
  14. Khianngam S., Tanasupawat S., Akaracharanya A., Kim K. K., Lee K. C., Lee J. S. ( 2010b ). Cohnella thailandensis sp. nov., a xylanolytic bacterium from Thai soil. . Int J Syst Evol Microbiol 60, 22842287. [View Article] [PubMed]
    [Google Scholar]
  15. Khianngam S., Tanasupawat S., Akaracharanya A., Kim K. K., Lee K. C., Lee J. S. ( 2012 ). Cohnella cellulosilytica sp. nov., isolated from buffalo faeces. . Int J Syst Evol Microbiol 62, 19211925. [View Article] [PubMed]
    [Google Scholar]
  16. Kim S. J., Weon H. Y., Kim Y. S., Anandham R., Jeon Y. A., Hong S. B., Kwon S.-W. ( 2010 ). Cohnella yongneupensis sp. nov. and Cohnella ginsengisoli sp. nov., isolated from two different soils. . Int J Syst Evol Microbiol 60, 526530. [View Article] [PubMed]
    [Google Scholar]
  17. Kim S. J., Weon H. Y., Kim Y. S., Kwon S.-W. ( 2011 ). Cohnella soli sp. nov. and Cohnella suwonensis sp. nov. Isolated from soil samples in Korea. . J Microbiol 49, 10331038. [View Article] [PubMed]
    [Google Scholar]
  18. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. & other authors ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716721. [View Article] [PubMed]
    [Google Scholar]
  19. Luo X., Wang Z., Dai J., Zhang L., Fang C. ( 2010 ). Cohnella damensis sp. nov., a motile xylanolytic bacteria isolated from a low altitude area in Tibet. . J Microbiol Biotechnol 20, 410414.[PubMed] [CrossRef]
    [Google Scholar]
  20. Mayilraj S., Ruckmani A., Kaur C., Kaur I., Klenk H. P. ( 2011 ). Cohnella ferri sp. nov. a novel member of the genus Cohnella isolated from haematite ore. . Curr Microbiol 62, 17041709. [CrossRef]
    [Google Scholar]
  21. Mesbah M., Premachandran U., Whitman W. B. ( 1989 ). Precise measurement of the G+C content of deoxyribonucleic acid by high performance liquid chromatography. . Int J Syst Bacteriol 39, 159167. [View Article]
    [Google Scholar]
  22. Rhuland L. E., Work E., Denman R. F., Hoare D. S. ( 1955 ). The behavior of the isomers of α,ϵ-diaminopimelic acid on paper chromatograms. . J Am Chem Soc 77, 48444846. [View Article]
    [Google Scholar]
  23. Shen P., Fan X. R., Li G. W. ( 1999 ). Experiment of Microbiology. Beijing:: Higher Education Press;.
    [Google Scholar]
  24. Shiratori H., Tagami Y., Beppu T., Ueda K. ( 2010 ). Cohnella fontinalis sp. nov., a xylanolytic bacterium isolated from fresh water. . Int J Syst Evol Microbiol 60, 13441348. [View Article] [PubMed]
    [Google Scholar]
  25. Stackebrandt E., Goebel B. M. ( 1994 ). Taxonomic note: a place for DNA–DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol 44, 846849. [View Article]
    [Google Scholar]
  26. Tindall B. J. ( 1990a ). A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. . Syst Appl Microbiol 13, 128130. [View Article]
    [Google Scholar]
  27. Tindall B. J. ( 1990b ). Lipid composition of Halobacterium lacusprofundi . . FEMS Microbiol Lett 66, 199202. [View Article]
    [Google Scholar]
  28. Wang L.-Y., Chen S.-F., Wang L., Zhou Y.-G., Liu H.-C. ( 2012 ). Cohnella plantaginis sp. nov., a novel nitrogen-fixing species isolated from plantain rhizosphere soil. . Antonie van Leeuwenhoek 102, 8389. [View Article] [PubMed]
    [Google Scholar]
  29. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. & other authors ( 1987 ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37, 463464. [View Article]
    [Google Scholar]
  30. Yoon J. H., Jung Y. T. ( 2012 ). Cohnella boryungensis sp. nov., isolated from soil. . Antonie van Leeuwenhoek 101, 769775. [View Article] [PubMed]
    [Google Scholar]
  31. Yoon M. H., Ten L. N., Im W. T. ( 2007 ). Cohnella panacarvi sp. nov., a xylanolytic bacterium isolated from ginseng cultivating soil. . J Microbiol Biotechnol 17, 913918.[PubMed]
    [Google Scholar]
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