A bacterial strain, JLT2016T, was isolated from a sample of South-eastern Pacific deep-sea water. Cells were Gram-stain-negative, aerobic, devoid of flagella, motile by gliding and rod-shaped. Colonies were mucoid and cream. Growth occurred at 1.0–11.0 % (w/v) NaCl, 10–40 °C and pH 4.0–9.0. The major fatty acids were summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c) (60.5 %), C19 : 0 cyclo ω8c (10.9 %) and C16 : 0 (9.0 %). The polar lipids included diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and two sphingoglycolipids. The DNA G+C content was 67.1 mol%. The closest relative of strain JLT2016T was Salipiger mucosus A3T (96.7 % 16S rRNA gene sequence similarity). The results of phylogenetic analyses with different treeing algorithms indicated that this strain belonged to the Roseobacterclade in the order Rhodobacterales. Based on polyphasic analysis, strain JLT2016T is considered to represent a novel genus and species, for which the name Thiobacimonas profunda gen. nov., sp. nov. is proposed. The type strain is JLT2016T ( = LMG 27365T = CGMCC 1.12377T).
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Funding
This study was supported by the:
National Key Basic Research Program of China
(Award 2013CB955700)
National Natural Science Foundation of China project
(Award 41276131 and 91028001)
ChoJ.-C.,
GiovannoniS. J.(2003).Parvularcula bermudensis gen. nov., sp. nov., a marine bacterium that forms a deep branch in the α-Proteobacteria
. . Int J Syst Evol Microbiol53, 1031–1036. [View Article][PubMed]
ChoJ. C.,
GiovannoniS. J.(2006).Pelagibaca bermudensis gen. nov., sp. nov., a novel marine bacterium within the Roseobacter clade in the order Rhodobacterales
. . Int J Syst Evol Microbiol56, 855–859. [View Article][PubMed]
CollinsM. D.,
GoodfellowM.,
MinnikinD. E.(1980). Fatty acid, isoprenoid quinone and polar lipid composition in the classification of Curtobacterium and related taxa. . J Gen Microbiol118, 29–37.[PubMed]
EmbleyT. M.(1991). The linear PCR reaction: a simple and robust method for sequencing amplified rRNA genes. . Lett Appl Microbiol13, 171–174. [View Article][PubMed]
FraserS. L.,
JorgensenJ. H.(1997). Reappraisal of the antimicrobial susceptibilities of Chryseobacterium and Flavobacterium species and methods for reliable susceptibility testing. . Antimicrob Agents Chemother41, 2738–2741.[PubMed]
GarrityG. M.,
BellJ. A.,
LilburnT.(2005). Order III. Rhodobacterales ord. nov.. In Bergey’s Manual of Systematic Bacteriology, , 2nd edn., vol. 2, p. 161. Edited by
BrennerD. J.,
KriegN. R.,
StaleyJ. T.,
GarrityG. M.
. New York:: Springer;.
GerhardtP.,
MurrayR. G. E.,
WoodW. A.,
KriegN. R.(editors) (1994).Methods for General and Molecular Bacteriology. Washington, DC:: American Society for Microbiology;.
GiovannoniS.,
RappéM.(2000). Evolution, diversity and molecular ecology of marine prokaryotes. . In Microbial Ecology of the Oceans, pp. 47–84. Edited by
KirchmanD. L.
. New York:: Wiley;.
HallT. A.(1999). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. . Nucleic Acids Symp Ser41, 95–98.
Martínez-CánovasM. J.,
QuesadaE.,
Martínez-ChecaF.,
del MoralA.,
BéjarV.(2004).Salipiger mucescens gen. nov., sp. nov., a moderately halophilic, exopolysaccharide-producing bacterium isolated from hypersaline soil, belonging to the α-Proteobacteria
. . Int J Syst Evol Microbiol54, 1735–1740. [View Article][PubMed]
PukallR.,
BuntefussD.,
FrühlingA.,
RohdeM.,
KroppenstedtR. M.,
BurghardtJ.,
LebaronP.,
BernardL.,
StackebrandtE.(1999).Sulfitobacter mediterraneus sp. nov., a new sulfite-oxidizing member of the α-Proteobacteria
. . Int J Syst Bacteriol49, 513–519. [View Article][PubMed]
RügerH. J.,
KrambeckH. J.(1994). Evaluation of the BIOLOG substratemetabolism system for classification of marine bacteria. . Syst Appl Microbiol17, 281–288. [View Article]
SmibertR. M.,
KriegN. R.(1994). Phenotypic characterization. . In Methods for General and Molecular Microbiology, pp. 611–654. Edited by
GerhardtP.,
MurrayR. G. E.,
WoodW. A.,
KriegN. R.
. Washington, DC:: American Society for Microbiology;.
TalaveraG.,
CastresanaJ.(2007). Improvement in phylogenies after removing divergent and ambiguously aligned blocks from protein sequence alignment. . Syst Biol56, 564–577. [CrossRef]
TanT. F.,
WangB. J.,
ShaoZ. Z.(2009).Donghicola xiamenensis sp. nov., a marine bacterium isolated from seawater of the Taiwan Strait in China. . Int J Syst Evol Microbiol59, 1143–1147. [View Article][PubMed]
TindallB. J.(1990a). A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. . Syst Appl Microbiol13, 128–130. [View Article]
ZechH.,
TholeS.,
SchreiberK.,
KalhöferD.,
VogetS.,
BrinkhoffT.,
SimonM.,
SchomburgD.,
RabusR.(2009). Growth phase-dependent global protein and metabolite profiles of Phaeobacter gallaeciensis strain DSM 17395, a member of the marine Roseobacter-clade. . Proteomics9, 3677–3697. [View Article][PubMed]