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Volume 65, Issue Pt_2

sp. nov., a moderately halophilic bacterium isolated from saltern soil Free

Abstract

A Gram-stain-positive, moderately halophilic bacterium, designated BH043, was isolated from saltern soil of Gomso in Korea. Cells were motile rods, producing ellipsoidal endospores at a terminal position in swollen sporangia. Strain BH043 was strictly aerobic, grew at pH 6.0–10.0 (optimal growth at pH 7.5), at 10–55 °C (optimal growth at 30 °C) and at salinities of 1–20 % (w/v) NaCl, growing optimally with 7 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequence indicated that strain BH043 belongs to the family and was most closely related to the type strains of the five recognized species of the genus , showing sequence similarity to Y32 (97.5 % similarity), BH030004 (97.4 %), BH030062 (97.0 %), JSM 072002 (96.4 %) and JSM 076056 (96.2 %). The major cellular fatty acids of strain BH043 were iso-C and anteiso-C. The genomic DNA G+C content was 42.5 mol%. The major isoprenoid quinone was MK-7 and -diaminopimelic acid was present in the cell-wall peptidoglycan as the diagnostic diamino acid. The major polar lipids were phosphatidylethanolamine and phosphatidylglycerol. DNA–DNA relatedness between strain BH043 and the type strains of other species of the genus , CGMCC 1.10680 and KCTC 3917 and KCTC 3890, was 35, 24 and 18 %, respectively. On the basis of polyphasic analysis from this study, strain BH043 represents a novel species of the genus for which the name sp. nov. is proposed. The type strain is BH043 ( = KACC 17607 = NBRC 109831 = NCAIM B.02529).

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Funding
This study was supported by the:
  • Regional SubGenBank Support Program of Rural Development Administration
© 2015 IUMS
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2015-02-01
2024-12-09
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Pontibacillus salicampi sp. nov., a moderately halophilic bacterium isolated from saltern soil
Int J Syst Evol Microbiol 65, 375 (2015); https://doi.org/10.1099/ijs.0.066423-0
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