1887

Abstract

During the study of bacteria associated with bats affected by white-nose syndrome hibernating in caves in the Czech Republic, we isolated two facultatively anaerobic, Gram-stain-negative bacteria, designated strains 12 and 52. Strains 12 and 52 were motile, rod-like bacteria (0.5–0.6 µm in diameter; 1–1.3 µm long), with optimal growth at 20–35 °C and pH 6–8. On the basis of the almost complete sequence of their 16S rRNA genes they should be classified within the genus ; the closest relatives to strains 12 and 52 were DSM 4597 (99.5 % similarity in 16S rRNA gene sequences) and DSM 4569 (99.5 % similarity in 16S rRNA gene sequences), respectively. DNA–DNA relatedness between strain 12 and DSM 4597 was only 37.1 % and between strain 52 and DSM 4569 was only 56.2 %. Both values are far below the 70 % threshold value for species delineation. In view of these data, we propose the inclusion of the two isolates in the genus as representatives of sp. nov. (type strain 12 = CECT 8594 = DSM 28726) and sp. nov. (type strain 52 = CECT 8595 = DSM 28727).

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2015-01-01
2019-08-24
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