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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 64, Issue Pt_12

gen. nov., sp. nov., isolated from the golden sea squirt Free

Abstract

A Gram-stain-negative, aerobic, non-motile and coccoid, ovoid or rod-shaped bacterial strain, designated RSS1-M3, was isolated from a golden sea squirt () collected from the East Sea, South Korea. Strain RSS1-M3 grew optimally at 30 °C, at pH 7.0–8.0 and in presence of 2.0 % (w/v) NaCl. Strain RSS1-M3 exhibited the highest 16S rRNA gene sequence similarity (96.55 %) to the type strain of . Neighbour-joining and maximum-likelihood phylogenetic trees based on 16S rRNA gene sequences revealed that strain RSS1-M3 clustered with the type strains, or proposed type strains, of , , and , showing sequence similarity of 94.88–96.32 %. Strain RSS1-M3 contained Q-10 as the predominant ubiquinone and Cω7 and C as the major fatty acids. The polar lipid profile of strain RSS1-M3, containing phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, one unidentified aminolipid and one unidentified lipid as major components, could be distinguished from those of the phylogenetically related genera. The DNA G+C content of strain RSS1-M3 was 55.8 mol%. On the basis of the phylogenetic, chemotaxonomic and phenotypic properties, strain RSS1-M3 is considered to represent a novel species of a new genus within the class , for which the name gen. nov., sp. nov. is proposed. The type strain is RSS1-M3 ( = KCTC 42118 = CECT 8599).

  • Published Online:
Funding
This study was supported by the:
  • National Fisheries Research and Development Institute (NFRDI)
  • Ministry of Science, ICT & Future Planning (MSIP) of the Republic of Korea (Award NRF-2013M3A9A5075953)
© 2014 IUMS
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2014-12-01
2025-03-15
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Ascidiaceihabitans donghaensis gen. nov., sp. nov., isolated from the golden sea squirt Halocynthia aurantium
Int J Syst Evol Microbiol 64, 3970 (2014); https://doi.org/10.1099/ijs.0.066399-0
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