1887

Abstract

A Gram-stain-negative, aerobic, non-motile and coccoid, ovoid or rod-shaped bacterial strain, designated RSS1-M3, was isolated from a golden sea squirt () collected from the East Sea, South Korea. Strain RSS1-M3 grew optimally at 30 °C, at pH 7.0–8.0 and in presence of 2.0 % (w/v) NaCl. Strain RSS1-M3 exhibited the highest 16S rRNA gene sequence similarity (96.55 %) to the type strain of . Neighbour-joining and maximum-likelihood phylogenetic trees based on 16S rRNA gene sequences revealed that strain RSS1-M3 clustered with the type strains, or proposed type strains, of , , and , showing sequence similarity of 94.88–96.32 %. Strain RSS1-M3 contained Q-10 as the predominant ubiquinone and Cω7 and C as the major fatty acids. The polar lipid profile of strain RSS1-M3, containing phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, one unidentified aminolipid and one unidentified lipid as major components, could be distinguished from those of the phylogenetically related genera. The DNA G+C content of strain RSS1-M3 was 55.8 mol%. On the basis of the phylogenetic, chemotaxonomic and phenotypic properties, strain RSS1-M3 is considered to represent a novel species of a new genus within the class , for which the name gen. nov., sp. nov. is proposed. The type strain is RSS1-M3 ( = KCTC 42118 = CECT 8599).

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2014-12-01
2019-11-15
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