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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 64, Issue Pt_12

Morphology, ontogenetic features and SSU rRNA gene-based phylogeny of a soil ciliate, spec. nov. (Protista, Ciliophora, Stichotrichia) Free

Abstract

The morphology, ontogeny and SSU rRNA gene-based phylogeny of spec. nov., isolated from the slightly saline soil of a mangrove wetland in Zhanjiang, southern China, were investigated. The novel species was characterized by having five to eight buccal cirri arranged in a row, three to five transverse cirri, four macronuclear nodules aligned, and 17–32 and 20–34 cirri in frontoventral rows V and VI, respectively, both extending to the transverse cirri. The main ontogenetic features of the novel species were as follows: (1) the parental adoral zone of the membranelles is completely inherited by the proter; (2) the frontoventral and transverse cirri are formed in a six-anlagen mode; (3) basically, the frontal-ventral-transverse cirral anlagen II–V generate one transverse cirrus each at their posterior ends, while anlage VI provides no transverse cirrus; (4) both marginal rows and dorsal kineties develop intrakinetally, no dorsal kinety fragment is formed; and (5) the macronuclear nodules fuse into a single mass at the middle stage. Phylogenetic analyses based on the SSU rRNA gene showed that the novel species groups with the clade containing , , and two species of the genus . Given present knowledge, it was considered to be still too early to come to a final conclusion regarding the familial classification of the genus ; further investigations of key taxa with additional molecular markers are required.

  • Published Online:
Funding
This study was supported by the:
  • Natural Science Foundation of China (Award 31111120437 and 41176119, 31030059)
  • King Saud University, Deanship of Scientific Research, International Research Group Program (Award IRG14-22)
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2014-12-01
2024-04-20
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Morphology, ontogenetic features and SSU rRNA gene-based phylogeny of a soil ciliate, Bistichella cystiformans spec. nov. (Protista, Ciliophora, Stichotrichia)
Int J Syst Evol Microbiol 64, 4049 (2014); https://doi.org/10.1099/ijs.0.066381-0
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