1887

Abstract

Two Gram-stain-negative, non-motile, rod-shaped bacterial strains, designated BM5-7 and BM9-1 were isolated from soil of the root system of a mangrove forest. Phylogenetic analysis based on 16S rRNA gene sequences showed that the two isolates belong to the genus . The chemotaxonomic characteristics of these isolates included the presence of C cyclo ω8 and Cω7 as the major cellular fatty acids and Q-10 as the dominant ubiquinone. The genomic DNA G+C contents of strains BM5-7 and BM9-1 were 61.0 and 59.7 mol% (HPLC method), respectively. The 16S rRNA gene sequence similarity between the two strains was 98.1 %, but DNA–DNA hybridization indicated 44 % relatedness. Strains BM5-7 and BM9-1 exhibited 16S rRNA gene sequence similarities of 98.0–99.2 % and 97.7–98.1 %, respectively, with type strains of and . Combined data from phenotypic, phylogenetic and DNA–DNA relatedness studies demonstrated that strains BM5-7 and BM9-1 are representatives of two novel species of the genus , for which the names sp. nov. (type strain BM5-7 = DSM 28101 = LMG 27958) and sp. nov. (type strain BM9-1 = DSM 28102 = LMG 27959) are proposed.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.066373-0
2014-09-01
2019-11-18
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/64/9/3104.html?itemId=/content/journal/ijsem/10.1099/ijs.0.066373-0&mimeType=html&fmt=ahah

References

  1. Bibi F., Chung E. J., Khan A., Jeon C. O., Chung Y. R.. ( 2013;). Martelella endophytica sp. nov., an antifungal bacterium associated with a halophyte. . Int J Syst Evol Microbiol 63:, 2914–2919. [CrossRef][PubMed]
    [Google Scholar]
  2. Cleenwerck I., Vandemeulebroecke K., Janssens D., Swings J.. ( 2002;). Re-examination of the genus Acetobacter, with descriptions of Acetobacter cerevisiae sp. nov. and Acetobacter malorum sp. nov.. Int J Syst Evol Microbiol 52:, 1551–1558. [CrossRef][PubMed]
    [Google Scholar]
  3. Collins M. D.. ( 1985;). Isoprenoid quinone analysis in classification and identification. . In Chemical Methods in Bacterial Systematics, pp. 267–287. Edited by Goodfellow M., Minnikin D. E... London:: Academic Press;.
    [Google Scholar]
  4. Ezaki T., Hashimoto Y., Yabuuchi E.. ( 1989;). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39:, 224–229. [CrossRef]
    [Google Scholar]
  5. Felsenstein, J. (2009). phylip (phylogeny inference package) version 3.69. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
  6. Goris J., Suzuki K., De Vos P., Nakase T., Kersters K.. ( 1998;). Evaluation of a microplate DNA-DNA hybridization method compared with the initial renaturation method. . Can J Microbiol 44:, 1148–1153. [CrossRef]
    [Google Scholar]
  7. Kim O.-S., Cho Y.-J., Lee K., Yoon S.-H., Kim M., Na H., Park S.-C., Jeon Y. S., Lee J.-H.. & other authors ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62:, 716–721. [CrossRef][PubMed]
    [Google Scholar]
  8. Mesbah M., Premachandran U., Whitman W. B.. ( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39:, 159–167. [CrossRef]
    [Google Scholar]
  9. Rivas R., Sánchez-Márquez S., Mateos P. F., Martínez-Molina E., Velázquez E.. ( 2005;). Martelella mediterranea gen. nov., sp. nov., a novel α-proteobacterium isolated from a subterranean saline lake. . Int J Syst Evol Microbiol 55:, 955–959. [CrossRef][PubMed]
    [Google Scholar]
  10. Saitou N., Nei M.. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  11. Sambrook J., Frisch E. F., Maniatis T.. ( 1989;). Molecular Cloning: a Laboratory Manual, , 2nd edn.. Cold Spring Harbor, NY:: Cold Spring Harbor Laboratory;.
    [Google Scholar]
  12. Sasser, M. (1990). Identification of bacteria by gas chromatography of cellular fatty acids MIDI Technical Note 101. Newark, DE: MIDI Inc.
  13. Süßmuth R., Eberspächer J., Haag R., Springer W.. ( 1987;). Biochemisch-mikrobiologisches Praktikum. Stuttgart:: Georg Thieme Verlag;.
    [Google Scholar]
  14. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S.. ( 2013;). mega6: molecular evolutionary genetics analysis version 6.0. . Mol Biol Evol 30:, 2725–2729. [CrossRef][PubMed]
    [Google Scholar]
  15. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G.. ( 1997;). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25:, 4876–4882. [CrossRef][PubMed]
    [Google Scholar]
  16. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E.. & other authors ( 1987;). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37:, 463–464. [CrossRef]
    [Google Scholar]
  17. Wu C., Lu X., Qin M., Wang Y., Ruan J.. ( 1989;). Analysis of menaquinone compound in microbial cells by HPLC. . Microbiology [English translation of Microbiology (Beijing)] 16:, 176–178 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.066373-0
Loading
/content/journal/ijsem/10.1099/ijs.0.066373-0
Loading

Data & Media loading...

Supplements

Supplementary Material 

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error