1887

Abstract

A pink-pigmented, non-motile, coccoid bacterial strain, designated G3-6-20, was isolated from a soil sample collected in the Grove Mountains, East Antarctica. This strain was resistant to UV irradiation (810 J m) and slightly more sensitive to desiccation as compared with . Phylogenetic analyses based on the 16S rRNA gene sequence of the isolate indicated that the organism belongs to the genus . Highest sequence similarities were with CC-FR2-10 (93.5 %), X-82 (92.8 %), Wt/1a (92.5 %), MJ27 (92.3 %), R-12 (92.3 %), PB314 (92.2 %) and DSM 20539 (92.2 %). Major fatty acids were Cω7, summed feature 3 (Cω7 and/or Cω6), anteiso-C and C. The G+C content of the genomic DNA of strain G3-6-20 was 63.1 mol%. Menaquinone 8 (MK-8) was the predominant respiratory quinone. Based on its phylogenetic position, and chemotaxonomic and phenotypic characteristics, strain G3-6-20 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is G3-6-20 ( = DSM 27864 = CCTCC AB 2013263).

Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award CHINARE2013-04-01, 41276175 and CHINARE2013-02-01)
  • National Programs for High Technology Research and Development of China (Award 2012AA021706)
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2015-02-01
2021-04-17
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References

  1. Asker D., Awad T. S., Beppu T., Ueda K. ( 2009 ). Deinococcus aquiradiocola sp. nov., isolated from a radioactive site in Japan. . Int J Syst Evol Microbiol 59, 144149. [CrossRef] [PubMed]
    [Google Scholar]
  2. Brooks B. W., Murray R. G. E. ( 1981 ). Nomenclature for “Micrococcus radiodurans” and other radiation-resistant cocci: Deinococcaceae fam. nov. and Deinococcus gen. nov., including five species. . Int J Syst Bacteriol 31, 353360. [CrossRef]
    [Google Scholar]
  3. Buck J. D. ( 1982 ). Nonstaining (KOH) method for determination of gram reactions of marine bacteria. . Appl Environ Microbiol 44, 992993.[PubMed]
    [Google Scholar]
  4. Callegan R. P., Nobre M. F., McTernan P. M., Battista J. R., Navarro-González R., McKay C. P., da Costa M. S., Rainey F. A. ( 2008 ). Description of four novel psychrophilic, ionizing radiation-sensitive Deinococcus species from alpine environments. . Int J Syst Evol Microbiol 58, 12521258. [CrossRef] [PubMed]
    [Google Scholar]
  5. Chen W., Wang B.-J., Hong H.-Z., Yang H., Liu S.-J. ( 2012 ). Deinococcus reticulitermitis sp. nov., isolated from a termite gut. . Int J Syst Evol Microbiol 62, 7883. [CrossRef] [PubMed]
    [Google Scholar]
  6. Convey P., Gibson J. A. E., Hillenbrand C. D., Hodgson D. A., Pugh P. J. A., Smellie J. L., Stevens M. I. ( 2008 ). Antarctic terrestrial life – challenging the history of the frozen continent?. Biol Rev Camb Philos Soc 83, 103117. [CrossRef] [PubMed]
    [Google Scholar]
  7. de Groot A., Chapon V., Servant P., Christen R., Saux M. F., Sommer S., Heulin T. ( 2005 ). Deinococcus deserti sp. nov., a gamma-radiation-tolerant bacterium isolated from the Sahara Desert. . Int J Syst Evol Microbiol 55, 24412446. [CrossRef] [PubMed]
    [Google Scholar]
  8. Felsenstein J. ( 2009 ). phylip (phylogeny inference package), version 3.69. Distributed by the author. . Department of Genome Sciences, University of Washington, Seattle, USA;.
  9. Ferreira A. C., Nobre M. F., Rainey F. A., Silva M. T., Wait R., Burghardt J., Chung A. P., da Costa M. S. ( 1997 ). Deinococcus geothermalis sp. nov. and Deinococcus murrayi sp. nov., two extremely radiation-resistant and slightly thermophilic species from hot springs. . Int J Syst Bacteriol 47, 939947. [CrossRef] [PubMed]
    [Google Scholar]
  10. Hirsch P., Gallikowski C. A., Siebert J., Peissl K., Kroppenstedt R., Schumann P., Stackebrandt E., Anderson R. ( 2004 ). Deinococcus frigens sp. nov., Deinococcus saxicola sp. nov., and Deinococcus marmoris sp. nov., low temperature and draught-tolerating, UV-resistant bacteria from continental Antarctica. . Syst Appl Microbiol 27, 636645. [CrossRef] [PubMed]
    [Google Scholar]
  11. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. & other authors ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716721. [CrossRef] [PubMed]
    [Google Scholar]
  12. Lai W.-A., Kämpfer P., Arun A. B., Shen F.-T., Huber B., Rekha P. D., Young C.-C. ( 2006 ). Deinococcus ficus sp. nov., isolated from the rhizosphere of Ficus religiosa L.. Int J Syst Evol Microbiol 56, 787791. [CrossRef] [PubMed]
    [Google Scholar]
  13. Lester E. D., Satomi M., Ponce A. ( 2007 ). Microflora of extreme arid Atacama Desert soils. . Soil Biol Biochem 39, 704708. [CrossRef]
    [Google Scholar]
  14. Li H.-R., Yu Y., Luo W., Zeng Y.-X. ( 2010 ). Marisediminicola antarctica gen. nov., sp. nov., an actinobacterium isolated from the Antarctic. . Int J Syst Evol Microbiol 60, 25352539. [CrossRef] [PubMed]
    [Google Scholar]
  15. Mesbah M., Premachandran U., Whitman W. B. ( 1989 ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39, 159167. [CrossRef]
    [Google Scholar]
  16. Oyaizu H., Stackebrandt E., Schleifer K. H., Ludwig W., Pohla H., Ito H., Hirata A., Oyaizu Y., Komagata K. ( 1987 ). A radiation-resistant rod-shaped bacterium, Deinococcus grandis gen. nov., sp. nov., with peptidoglycan containing ornithine. . Int J Syst Bacteriol 37, 6267. [CrossRef]
    [Google Scholar]
  17. Peng F., Zhang L., Luo X.-S., Dai J., An H.-L., Tang Y.-L., Fang C.-X. ( 2009 ). Deinococcus xinjiangensis sp. nov., isolated from desert soil. . Int J Syst Evol Microbiol 59, 709713. [CrossRef] [PubMed]
    [Google Scholar]
  18. Rainey F. A., Ray K., Ferreira M., Gatz B. Z., Nobre M. F., Bagaley D., Rash B. A., Park M. J., Earl A. M. & other authors ( 2005 ). Extensive diversity of ionizing-radiation-resistant bacteria recovered from Sonoran Desert soil and description of nine new species of the genus Deinococcus obtained from a single soil sample. . Appl Environ Microbiol 71, 52255235. [CrossRef] [PubMed]
    [Google Scholar]
  19. Schleifer K. H., Kandler O. ( 1972 ). Peptidoglycan types of bacterial cell walls and their taxonomic implications. . Bacteriol Rev 36, 407477.[PubMed]
    [Google Scholar]
  20. Smibert R. M., Krieg N. R. ( 1994 ). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  21. Srinivasan S., Lee J.-J., Lim S., Joe M., Kim M. K. ( 2012a ). Deinococcus humi sp. nov., isolated from soil. . Int J Syst Evol Microbiol 62, 28442850. [CrossRef] [PubMed]
    [Google Scholar]
  22. Srinivasan S., Kim M. K., Lim S., Joe M., Lee M. ( 2012b ). Deinococcus daejeonensis sp. nov., isolated from sludge in a sewage disposal plant. . Int J Syst Evol Microbiol 62, 12651270. [CrossRef] [PubMed]
    [Google Scholar]
  23. Suresh K., Reddy G. S., Sengupta S., Shivaji S. ( 2004 ). Deinococcus indicus sp. nov., an arsenic-resistant bacterium from an aquifer in West Bengal, India. . Int J Syst Evol Microbiol 54, 457461. [CrossRef] [PubMed]
    [Google Scholar]
  24. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 27312739. [CrossRef] [PubMed]
    [Google Scholar]
  25. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. ( 1997 ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25, 48764882. [CrossRef] [PubMed]
    [Google Scholar]
  26. Tindall B. J. ( 1990 ). Lipid composition of Halobacterium lacusprofundi . . FEMS Microbiol Lett 66, 199202. [CrossRef]
    [Google Scholar]
  27. Wang W., Mao J., Zhang Z.-D., Tang Q.-Y., Xie Y.-Q., Zhu J., Zhang L.-X., Liu Z.-H., Shi Y.-H., Goodfellow M. ( 2010 ). Deinococcus wulumuqiensis sp. nov., and Deinococcus xibeiensis sp. nov., isolated from radiation-polluted soil. . Int J Syst Evol Microbiol 60, 20062010. [CrossRef] [PubMed]
    [Google Scholar]
  28. Weon H.-Y., Kim B.-Y., Schumann P., Son J.-A., Jang J., Go S.-J., Kwon S.-W. ( 2007 ). Deinococcus cellulosilyticus sp. nov., isolated from air. . Int J Syst Evol Microbiol 57, 16851688. [CrossRef] [PubMed]
    [Google Scholar]
  29. Xie C. H., Yokota A. ( 2003 ). Phylogenetic analyses of Lampropedia hyalina based on the 16S rRNA gene sequence. . J Gen Appl Microbiol 49, 345349. [CrossRef] [PubMed]
    [Google Scholar]
  30. Yang Y., Itoh T., Yokobori S.-i., Itahashi S., Shimada H., Satoh K., Ohba H., Narumi I., Yamagishi A. ( 2009 ). Deinococcus aerius sp. nov., isolated from the high atmosphere. . Int J Syst Evol Microbiol 59, 18621866. [CrossRef] [PubMed]
    [Google Scholar]
  31. Zhang Y.-Q., Sun C.-H., Li W.-J., Yu L.-Y., Zhou J.-Q., Zhang Y.-Q., Xu L.-H., Jiang C.-L. ( 2007 ). Deinococcus yunweiensis sp. nov., a gamma- and UV-radiation-resistant bacterium from China. . Int J Syst Evol Microbiol 57, 370375. [CrossRef] [PubMed]
    [Google Scholar]
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