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Abstract

A bacterial strain designated RKEM611 was isolated from the octocoral , collected off the coast of San Salvador, The Bahamas. The strain is Gram-stain-negative, an obligate aerobe, and pleomorphic. It requires NaCl for growth and exhibits optimal growth at 1–2 % (w/v) NaCl, 30–37 °C and pH 6.0–8.0. The predominant cellular fatty acids are Cω5 and C; the major respiratory quinone is menaquinone MK-6, and the DNA G+C content is 46.3 mol%. Based on phylogenetic analysis of the 16S rRNA gene, in addition to phenotypic characteristics, RKEM611 represents a novel species and genus of a novel family within the order . The names fam. nov. and gen., nov., sp., nov. are proposed. Isolate RKEM611 ( = NCCB 100521 = LMG 28452) is the type strain.

Funding
This study was supported by the:
  • , Canadian Research Chair
  • , NSERC
  • , Jeanne and Jean-Louis Lévesgue Foundation
  • , Canadian Foundation for Innovation
  • , Atlantic Canada Opportunities Agency
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2015-02-01
2020-09-28
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References

  1. Altschul S. F., Madden T. L., Schäffer A. A., Zhang J., Zhang Z., Miller W., Lipman D. J. ( 1997 ). Gapped blast and psi-blast: a new generation of protein database search programs. . Nucleic Acids Res 25, 33893402. [CrossRef] [PubMed]
    [Google Scholar]
  2. Baer M. L., Ravel J., Chun J., Hill R. T., Williams H. N. ( 2000 ). A proposal for the reclassification of Bdellovibrio stolpii and Bdellovibrio starrii into a new genus, Bacteriovorax gen. nov. as Bacteriovorax stolpii comb. nov. and Bacteriovorax starrii comb. nov., respectively. . Int J Syst Evol Microbiol 50, 219224. [CrossRef] [PubMed]
    [Google Scholar]
  3. Baer M. L., Ravel J., Piñeiro S. A., Guether-Borg D., Williams H. N. ( 2004 ). Reclassification of salt-water Bdellovibrio sp. as Bacteriovorax marinus sp. nov. and Bacteriovorax litoralis sp. nov.. Int J Syst Evol Microbiol 54, 10111016. [CrossRef] [PubMed]
    [Google Scholar]
  4. Bauer A. W., Kirby W. M. M., Sherris J. C., Turck M. ( 1966 ). Antibiotic susceptibility testing by a standardized single disk method. . Am J Clin Pathol 45, 493496.[PubMed]
    [Google Scholar]
  5. Chen H., Young S., Berhane T. K., Williams H. N. ( 2012 ). Predatory Bacteriovorax communities ordered by various prey species. . PLoS ONE 7, e34174. [CrossRef] [PubMed]
    [Google Scholar]
  6. Davidov Y., Jurkevitch E. ( 2004 ). Diversity and evolution of Bdellovibrio-and-like organisms (BALOs), reclassification of Bacteriovorax starrii as Peredibacter starrii gen. nov., comb. nov., and description of the Bacteriovorax-Peredibacter clade as Bacteriovoracaceae fam. nov. . Int J Syst Evol Microbiol 54, 14391452. [CrossRef] [PubMed]
    [Google Scholar]
  7. Eck R. V., Dayhoff M. O. ( 1966 ). Atlas of Protein Sequence and Structure. Silver Spring, MD:: National Biomedical Research Foundation;.
    [Google Scholar]
  8. Edwards U., Rogall T., Blöcker H., Emde M., Böttger E. C. ( 1989 ). Isolation and direct complete nucleotide determination of entire genes. Characterization of a gene coding for 16S ribosomal RNA. . Nucleic Acids Res 17, 78437853. [CrossRef] [PubMed]
    [Google Scholar]
  9. Felsenstein J. ( 1985 ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39, 783791. [CrossRef]
    [Google Scholar]
  10. Garrity G. M., Bell J. A., Lilburn T. ( 2005 ). Order VII. Bdellovibrionales . . In Bergey’s Manual of Systematic Bacteriology, , 2nd edn., vol. 2, pp. 10401058. Edited by Brenner D. J., Krieg N. R., Staley J. T., Garrity G. M. . New York:: Springer;.
    [Google Scholar]
  11. Gontang E. A., Fenical W., Jensen P. R. ( 2007 ). Phylogenetic diversity of gram-positive bacteria cultured from marine sediments. . Appl Environ Microbiol 73, 32723282. [CrossRef] [PubMed]
    [Google Scholar]
  12. Jukes T. H., Cantor C. R. ( 1969 ). Evolution of protein molecules. . In Mammalian Protein Metabolism, vol. 3, pp. 21132. Edited by Munro H. N. . New York:: Academic Press;. [CrossRef]
    [Google Scholar]
  13. Koval S. F., Hynes S. H., Flannagan R. S., Pasternak Z., Davidov Y., Jurkevitch E. ( 2013 ). Bdellovibrio exovorus sp. nov., a novel predator of Caulobacter crescentus . . Int J Syst Evol Microbiol 63, 146151. [CrossRef] [PubMed]
    [Google Scholar]
  14. Lane D. J. ( 1991 ). 16S/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 115175. Edited by Stackebrandt E., Goodfellow M. . New York:: Wiley;.
    [Google Scholar]
  15. Manefield M., Whiteley A. S., Griffiths R. I., Bailey M. J. ( 2002 ). RNA stable isotope probing, a novel means of linking microbial community function to phylogeny. . Appl Environ Microbiol 68, 53675373. [CrossRef] [PubMed]
    [Google Scholar]
  16. Marbach A., Varon M., Shilo M. ( 1975 ). Properties of marine bdellovibrios. . Microb Ecol 2, 284295. [CrossRef] [PubMed]
    [Google Scholar]
  17. Mesbah M., Premachandran U., Whitman W. B. ( 1989 ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39, 159167. [CrossRef]
    [Google Scholar]
  18. Miller L. T. ( 1982 ). Single derivatization method for routine analysis of bacterial whole-cell fatty acid methyl esters, including hydroxy acids. . J Clin Microbiol 16, 584586.[PubMed]
    [Google Scholar]
  19. Nei M., Kumar S. ( 2000 ). Molecular Evolution and Phylogenetics. New York:: Oxford University Press;.
    [Google Scholar]
  20. Piñeiro S. A., Williams H. N., Stine O. C. ( 2008 ). Phylogenetic relationships amongst the saltwater members of the genus Bacteriovorax using rpoB sequences and reclassification of Bacteriovorax stolpii as Bacteriolyticum stolpii gen. nov., comb. nov.. Int J Syst Evol Microbiol 58, 12031209. [CrossRef] [PubMed]
    [Google Scholar]
  21. Rzhetsky A., Nei M. ( 1992 ). A simple method for estimating and testing minimum-evolution trees. . Mol Biol Evol 9, 945967.
    [Google Scholar]
  22. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  23. Sasser M. ( 1990 ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. . Newark, DE:: MIDI Inc;.
  24. Seidler R. J., Starr M. P. ( 1969 ). Isolation and characterization of host-independent Bdellovibrios. . J Bacteriol 100, 769785.[PubMed]
    [Google Scholar]
  25. Seidler R. J., Starr M. P., Mandel M. ( 1969 ). Deoxyribonucleic acid characterization of Bdellovibrios. . J Bacteriol 100, 786790.[PubMed]
    [Google Scholar]
  26. Seidler R. J., Mandel M., Baptist J. N. ( 1972 ). Molecular heterogeneity of the Bdellovibrios: evidence of two new species. . J Bacteriol 109, 209217.[PubMed]
    [Google Scholar]
  27. Snyder A. R., Williams H. N., Baer M. L., Walker K. E., Stine O. C. ( 2002 ). 16S rDNA sequence analysis of environmental Bdellovibrio-and-like organisms (BALO) reveals extensive diversity. . Int J Syst Evol Microbiol 52, 20892094. [CrossRef] [PubMed]
    [Google Scholar]
  28. Stolp H., Starr M. P. ( 1963 ). Bdellovibrio bacteriovorus gen. et sp. n., a predatory ectoparasitic, and bacteriolytic microorganism. . Antonie van Leeuwenhoek 29, 217248. [CrossRef] [PubMed]
    [Google Scholar]
  29. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. ( 2013 ). mega6: molecular evolutionary genetics analysis version 6.0. . Mol Biol Evol 30, 27252729. [CrossRef] [PubMed]
    [Google Scholar]
  30. Tindall B. J. ( 1990a ). A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. . Syst Appl Microbiol 13, 128130. [CrossRef]
    [Google Scholar]
  31. Tindall B. J. ( 1990b ). Lipid composition of Halobacterium lacusprofundi . . FEMS Microbiol Lett 66, 199202. [CrossRef]
    [Google Scholar]
  32. Williams H. N., Falkler W. A. Jr ( 1984 ). Distribution of bdellovibrios in the water column of an estuary. . Can J Microbiol 30, 971974. [CrossRef] [PubMed]
    [Google Scholar]
  33. Zuker M. ( 2003 ). Mfold web server for nucleic acid folding and hybridization prediction. . Nucleic Acids Res 31, 34063415. [CrossRef] [PubMed]
    [Google Scholar]
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