1887

Abstract

A novel anaerobic propionigenic bacterium, strain ADRI, was isolated from sediment of an acid rock drainage environment (Tinto River, Spain). Cells were small (0.4–0.6×1–1.7 µm), non-motile and non-spore-forming rods. Cells possessed a Gram-negative cell-wall structure and were vancomycin-resistant. Strain ADRI utilized yeast extract and various sugars as substrates and formed propionate, lactate and acetate as major fermentation products. The optimum growth temperature was 30 °C and the optimum pH for growth was pH 6.5, but strain ADRI was able to grow at a pH as low as 3.0. Oxidase, indole formation, and urease and catalase activities were negative. Aesculin and gelatin were hydrolysed. The predominant cellular fatty acids of strain ADRI were anteiso-C (30.3 %), iso-C (29.2 %) and iso-C 3-OH (14.9 %). Major menaquinones were MK-8 (52 %) and MK-9 (48 %). The genomic DNA G+C content was 39.9 mol%. Phylogenetically, strain ADRI was affiliated to the family of the phylum . The most closely related cultured species were with 16S rRNA gene sequence similarity of 87.5 % and several species of the genus (similarities of 83.5–85.4 % to the type strains). Based on the distinctive ecological, phenotypic and phylogenetic characteristics of strain ADRI, a novel genus and species, gen. nov., sp. nov., is proposed. The type strain is ADRI ( = JCM 19374 = DSM 27471).

Funding
This study was supported by the:
  • European Research Council (Award 323009)
  • Netherlands Ministry of Education, Culture and Science
  • Netherlands Science Foundation (NWO) (Award 024.002.002)
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2014-12-01
2021-10-19
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