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Abstract

Strain LTF Kr01, a novel mesophilic, anaerobic, halotolerant, rod-shaped bacterium, was isolated from a drain at the bottom of a corroded kerosene storage tank of the Société Tunisienne des Industries de Raffinage (STIR), Bizerte, northern Tunisia. Cells were Gram-positive-staining rods, occurred singly or in pairs, and were motile by one lateral flagellum. Strain LTF Kr01 grew at temperatures between 15 and 40 °C (optimum 30 °C), between pH 5.5 and 8.2 (optimum pH 7.2) and at NaCl concentrations between 0 and 50 g l (optimum 5 g l). It reduced thiosulfate and elemental sulfur into sulfide, but did not reduce sulfate or sulfite. It utilized a wide range of carbohydrates (cellobiose, -glucose, -fructose, -mannitol, -ribose, sucrose, -xylose, maltose, -galactose, starch and trehalose) and produced acetate, CO and H as end products from glucose fermentation. The DNA G+C content was 37.4 mol%. The predominant cellular fatty acids were C and C. Phylogenetic analysis of the 16S rRNA gene sequence suggested that was the closest relative of strain LTF Kr01 (gene sequence similarity of 94.6 %). Based on phenotypic, phylogenetic and genotypic taxonomic characteristics, strain LTF Kr01 is proposed to represent a novel species of the genus , order , for which the name sp. nov. is proposed. The type strain is LTF Kr01 ( = DSM 28034 = JCM 19376).

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2015-01-01
2019-11-22
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