1887

Abstract

Five Gran-stain-negative, facultatively anaerobic, motile, bacterial strains were isolated from symptomatic bark tissue of canker. Strains grew at 4–41 °C, pH 4–10 and 0–6 % (w/v) salinity. They were positive with respect to catalase activity and negative for oxidase activity, nitrate reduction and the Voges–Proskauer reaction. Analysis of 16S rRNA gene sequences indicated that these five poplar isolates belong to the genus , having highest sequence similarity of 95.98 % with LMG 26270. These five isolates formed a single cluster based on multilocus sequence analysis, indicating that they all belong to a single taxon within the genus , which was confirmed by DNA–DNA hybridization. The DNA G+C content was 54.9–55.7 mol%, and the main fatty acids were C, Cω7, C cyclo and Cω7/iso-C 2-OH. Based on these results, we describe a novel species of the genus with the proposed name sp. nov. The type strain is D9-5 ( = CFCC 11963 = KCTC 42088).

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2015-02-01
2019-10-15
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