1887

Abstract

A novel, aerobic, heterotrophic, orange-pigmented, Gram-staining-negative, rod-shaped, gliding bacterial strain, designated JLT2000, was isolated from surface water of the South China Sea. The strain was oxidase- and catalase-positive. The major cellular fatty acids of strain JLT2000 were C, iso-C G, iso-C, iso-C 3-OH, summed feature 3 (comprising Cω7c and/or Cω6), C and C. MK-7 was the major respiratory quinone and the major polar lipids were phosphatidylcholine and phosphatidylethanolamine. The genomic DNA G+C content of strain JLT2000 was 37.9 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain JLT2000 formed a branch within the genus , but was clearly separated from the two established species of this genus, and . The 16S rRNA gene sequence similarity of strain JLT2000 with the type strains of these two species was 95.8 % and 96.1 %, respectively. Strain JLT2000 had a shorter cell length and wider growth range in different temperatures and salinities than those of NBRC 15989 and NBRC 15983. In addition, strain JLT2000 could utilize more carbon sources and hydrolyse more polymers than NBRC 15989 and NBRC 15983. Based on this polyphasic analysis, strain JLT2000 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is JLT2000 ( = JCM 18012 = CGMCC 1.10832).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.066027-0
2015-02-01
2019-10-23
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/2/452.html?itemId=/content/journal/ijsem/10.1099/ijs.0.066027-0&mimeType=html&fmt=ahah

References

  1. Andrews J. M..BSAC Working Party on Susceptibility Testing ( 2008;). BSAC standardized disc susceptibility testing method (version 7). . J Antimicrob Chemother 62:, 256–278. [CrossRef][PubMed]
    [Google Scholar]
  2. Bernardet J. F., Nakagawa Y., Holmes B..Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes ( 2002;). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. . Int J Syst Evol Microbiol 52:, 1049–1070. [CrossRef][PubMed]
    [Google Scholar]
  3. Bowman J. P.. ( 2000;). Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov.. Int J Syst Evol Microbiol 50:, 1861–1868.[PubMed]
    [Google Scholar]
  4. Cappuccino J. G., Sherman N.. ( 2002;). Microbiology: a laboratory Manual, , 6th edn.. Menlo Park,CA:: Benjamin/Cummings;.
    [Google Scholar]
  5. Dong X.-Z., Cai M.-Y.. ( 2001;). Determinative manual for routine bacteriology. Peking:: Scientific Press;.
    [Google Scholar]
  6. Fautz E., Reichenbach H.. ( 1980;). A simple test for flexirubin-type pigments. . FEMS Microbiol Lett 8:, 87–91. [CrossRef]
    [Google Scholar]
  7. Fraser S. L., Jorgensen J. H.. ( 1997;). Reappraisal of the antimicrobial susceptibilities of Chryseobacterium and Flavobacterium species and methods for reliable susceptibility testing. . Antimicrob Agents Chemother 41:, 2738–2741.[PubMed]
    [Google Scholar]
  8. Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R.. ( 1994;). Methods for General and Molecular Bacteriology. Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  9. Harrison A. P. Jr. ( 1984;). The acidophilic thiobacilli and other acidophilic bacteria that share their habitat. . Annu Rev Microbiol 38:, 265–292. [CrossRef][PubMed]
    [Google Scholar]
  10. Kates M.. ( 1986;). Techniques of Lipidology: Isolation, Analysis and Identification of Lipids (Laboratory Techniques in Biochemistry and Molecular Biology), , 2nd edn., vol. 3. Amsterdam:: Elsevier;.
    [Google Scholar]
  11. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H.. & other authors ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62:, 716–721. [CrossRef][PubMed]
    [Google Scholar]
  12. Komagata K., Suzuki K.. ( 1987;). Lipid and cell-wall analysis in bacterial systematics. . Methods Microbiol 19:, 161–207. [CrossRef]
    [Google Scholar]
  13. Lane D. J.. ( 1991;). 16S/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–175. Edited by Stackebrandt E., Goodfellow M... NY:: John Wiley & Sons;.
    [Google Scholar]
  14. Larkin M. A., Blackshields G., Brown N. P., Chenna R., McGettigan P. A., McWilliam H., Valentin F., Wallace I. M., Wilm A.. & other authors ( 2007;). Clustal W and Clustal X version 2.0. . Bioinformatics 23:, 2947–2948. [CrossRef][PubMed]
    [Google Scholar]
  15. Leadbetter E. R.. ( 1974;). Genus II Flexibacter Soriano 1945, Lewin 1969, 192 emend. mut. char.. In Bergey’s Manual of Determinative Bacteriology, , 8th edn., pp. 105–107. Edited by Buchanan R. E., Gibbons N. E... Baltimore:: Williams & Wilkins;.
    [Google Scholar]
  16. Lewin R. A.. ( 1969;). A classification of flexibacteria. . J Gen Microbiol 58:, 189–206. [CrossRef][PubMed]
    [Google Scholar]
  17. Ludwig, W., Euzéby, J. & Whitman, W. B. (2008). Draft taxonomic outline of the Bacteroidetes, Planctomycetes, Chlamydiae, Spiroehaetes, Fibrobacteres, Fusobacteria, Acidobacteria, Verrucomicrobia, Dictyoglomi, and Gemmatimonadetes. http://www.bergeys.org/outlines.html
  18. Mesbah M., Premachandran U., Whitman W. B.. ( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39:, 159–167. [CrossRef]
    [Google Scholar]
  19. Nakagawa Y., Sakane T., Suzuki M., Hatano K.. ( 2002;). Phylogenetic structure of the genera Flexibacter, Flexithrix, and Microscilla deduced from 16S rRNA sequence analysis. . J Gen Appl Microbiol 48:, 155–165. [CrossRef][PubMed]
    [Google Scholar]
  20. Nedashkovskaya O. I., Vancanneyt M., Kim S. B., Bae K. S.. ( 2010;). Reclassification of Flexibacter tractuosus (Lewin 1969) Leadbetter 1974 and ‘Microscilla sericea’ Lewin 1969 in the genus Marivirga gen. nov. as Marivirga tractuosa nom. rev., comb. nov. and Marivirga sericea nom. rev., comb. nov.. Int J Syst Evol Microbiol 60:, 1858–1863. [CrossRef][PubMed]
    [Google Scholar]
  21. Reichenbach H.. ( 1989;). Genus Flexibacter Soriano 1945, 92,AL emend. . In Bergey’s Manual of Systematic Bacteriology, vol. 3, pp. 2061–2071. Edited by Staley J. T., Bryant M. P., Pfennig N., Holt J. G... Baltimore:: Williams & Wilkins;.
    [Google Scholar]
  22. Smibert R. M., Krieg N. R.. ( 1994;). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R... Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  23. Sorokin D. Y., Banciu H., van Loosdrecht M., Kuenen J. G.. ( 2003;). Growth physiology and competitive interaction of obligately chemolithoautotrophic, haloalkaliphilic, sulfur-oxidizing bacteria from soda lakes. . Extremophiles 7:, 195–203.[PubMed]
    [Google Scholar]
  24. Suzuki M., Nakagawa Y., Harayama S., Yamamoto S.. ( 2001;). Phylogenetic analysis and taxonomic study of marine Cytophaga-like bacteria: proposal for Tenacibaculum gen. nov. with Tenacibaculum maritimum comb. nov. and Tenacibaculum ovolyticum comb. nov., and description of Tenacibaculum mesophilum sp. nov. and Tenacibaculum amylolyticum sp. nov.. Int J Syst Evol Microbiol 51:, 1639–1652. [CrossRef][PubMed]
    [Google Scholar]
  25. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S.. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739. [CrossRef][PubMed]
    [Google Scholar]
  26. Tindall B. J.. ( 1990a;). A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. . Syst Appl Microbiol 13:, 128–130. [CrossRef]
    [Google Scholar]
  27. Tindall B. J.. ( 1990b;). Lipid composition of Halobacterium lacusprofundi. . FEMS Microbiol Lett 66:, 199–202. [CrossRef]
    [Google Scholar]
  28. Yurkov V. V., Krieger S., Stackebrandt E., Beatty J. T.. ( 1999;). Citromicrobium bathyomarinum, a novel aerobic bacterium isolated from deep-sea hydrothermal vent plume waters that contains photosynthetic pigment-protein complexes. . J Bacteriol 181:, 4517–4525.[PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.066027-0
Loading
/content/journal/ijsem/10.1099/ijs.0.066027-0
Loading

Data & Media loading...

Supplements

Supplementary Data



PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error