1887

Abstract

Four Gram-stain-negative, aerobic, rod-shaped bacterial strains, MM-124, MM-126, NB-68 and NB-77, were isolated from the coastal seawater or a region with a bloom of sea sparkle around Geoje island in Korea. The sequence similarity values of the 16S rRNA gene between the isolates and DSM 12244 ranged from 97.7 to 98.2 %, and phylogenetic relationships suggested that they belong to a phylogenetic branch that includes the genera and . The isoprenoid quinone of all three novel strains was ubiquinone-10 and the major fatty acid was -vaccenic acid, as in other species of the genus . However, there were several differences in the morphological, physiological and biochemical characteristics among the four strains and the reference species of the genus . Moreover, the average nucleotide identity values between the three sequenced isolates and the reference strains were below 76.33, indicating that genomic variation exists between the isolates and reference strains. Chemotaxonomic characteristics together with phylogenetic affiliations and genomic distances illustrate that strains MM-124, NB-68 and NB-77 represent novel species of the genus , for which the names g sp. nov. (type strain MM-124 = KCTC 32124 = JCM 18835), sp. nov. (type strain NB-68 = KCTC 32122 = JCM 18833) and sp. nov. (type strain NB-77 = KCTC 32123 = JCM 18834) are proposed.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.065961-0
2014-11-01
2019-11-21
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/64/11/3760.html?itemId=/content/journal/ijsem/10.1099/ijs.0.065961-0&mimeType=html&fmt=ahah

References

  1. Berresheim H., Eisele F. L., Tanner D. J., Mcinnes L. M., Ramsey-Bell D. C., Covert D. S.. ( 1993;). Atmospheric sulfur chemistry and cloud condensation nuclei (CCN) concentrations over the northeastern pacific coast. . J Geophys Res-Atmos 98: (D7), 12701–12711. [CrossRef]
    [Google Scholar]
  2. Brinkhoff T., Giebel H. A., Simon M.. ( 2008;). Diversity, ecology, and genomics of the Roseobacter clade: a short overview. . Arch Microbiol 189:, 531–539. [CrossRef][PubMed]
    [Google Scholar]
  3. Buchan A., González J. M., Moran M. A.. ( 2005;). Overview of the marine roseobacter lineage. . Appl Environ Microbiol 71:, 5665–5677. [CrossRef][PubMed]
    [Google Scholar]
  4. Camelo-Castillo A., Benítez-Páez A., Belda-Ferre P., Cabrera-Rubio R., Mira A.. ( 2014;). Streptococcus dentisani sp. nov., a novel member of the mitis group. . Int J Syst Evol Microbiol 64:, 60–65. [CrossRef][PubMed]
    [Google Scholar]
  5. Chun J., Lee J. H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y. W.. ( 2007;). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. . Int J Syst Evol Microbiol 57:, 2259–2261. [CrossRef][PubMed]
    [Google Scholar]
  6. Curson A. R. J., Rogers R., Todd J. D., Brearley C. A., Johnston A. W. B.. ( 2008;). Molecular genetic analysis of a dimethylsulfoniopropionate lyase that liberates the climate-changing gas dimethylsulfide in several marine alpha-proteobacteria and Rhodobacter sphaeroides. . Environ Microbiol 10:, 757–767. [CrossRef][PubMed]
    [Google Scholar]
  7. Durán D., Rey L., Mayo J., Zúñiga-Dávila D., Imperial J., Ruiz-Argüeso T., Martínez-Romero E., Ormeño-Orrillo E.. ( 2014;). Bradyrhizobium paxllaeri sp. nov. and Bradyrhizobium icense sp. nov., nitrogen-fixing rhizobial symbionts of Lima bean (Phaseolus lunatus L.) in Peru. . Int J Syst Evol Microbiol 64:, 2072–2078. [CrossRef][PubMed]
    [Google Scholar]
  8. Goris J., Konstantinidis K. T., Klappenbach J. A., Coenye T., Vandamme P., Tiedje J. M.. ( 2007;). DNA-DNA hybridization values and their relationship to whole-genome sequence similarities. . Int J Syst Evol Microbiol 57:, 81–91. [CrossRef][PubMed]
    [Google Scholar]
  9. Ivanova E. P., Gorshkova N. M., Sawabe T., Zhukova N. V., Hayashi K., Kurilenko V. V., Alexeeva Y., Buljan V., Nicolau D. V.. & other authors ( 2004;). Sulfitobacter delicatus sp. nov. and Sulfitobacter dubius sp. nov., respectively from a starfish (Stellaster equestris) and sea grass (Zostera marina). . Int J Syst Evol Microbiol 54:, 475–480. [CrossRef][PubMed]
    [Google Scholar]
  10. Kim M., Oh H. S., Park S. C., Chun J.. ( 2014;). Towards a taxonomic coherence between average nucleotide identity and 16S rRNA gene sequence similarity for species demarcation of prokaryotes. . Int J Syst Evol Microbiol 64:, 346–351. [CrossRef][PubMed]
    [Google Scholar]
  11. Labrenz M., Tindall B. J., Lawson P. A., Collins M. D., Schumann P., Hirsch P.. ( 2000;). Staleya guttiformis gen. nov., sp. nov. and Sulfitobacter brevis sp. nov., alpha-3-Proteobacteria from hypersaline, heliothermal and meromictic antarctic Ekho Lake. . Int J Syst Evol Microbiol 50:, 303–313. [CrossRef][PubMed]
    [Google Scholar]
  12. Lander E. S., Waterman M. S.. ( 1988;). Genomic mapping by fingerprinting random clones: a mathematical analysis. . Genomics 2:, 231–239. [CrossRef][PubMed]
    [Google Scholar]
  13. Lucena T., Ruvira M. A., Macián M. C., Pujalte M. J., Arahal D. R.. ( 2014;). Roseovarius albus sp. nov., a new alphaproteobacterium isolated from the Mediterranean Sea. . Antonie van Leeuwenhoek 105:, 671–678. [CrossRef][PubMed]
    [Google Scholar]
  14. Ludwig W., Strunk O., Westram R., Richter L., Meier H., Yadhukumar, Buchner A., Lai T., Steppi S.. & other authors ( 2004;). arb: a software environment for sequence data. . Nucleic Acids Res 32:, 1363–1371. [CrossRef][PubMed]
    [Google Scholar]
  15. Minnikin D. E., Patel P. V., Alshamaony L., Goodfellow M.. ( 1977;). Polar lipid composition in the classification of Nocardia and related bacteria. . Int J Syst Evol Microbiol 27:, 104–117.
    [Google Scholar]
  16. Mou X. Z., Moran M. A., Stepanauskas R., González J. M., Hodson R. E.. ( 2005;). Flow-cytometric cell sorting and subsequent molecular analyses for culture-independent identification of bacterioplankton involved in dimethylsulfoniopropionate transformations. . Appl Environ Microbiol 71:, 1405–1416. [CrossRef][PubMed]
    [Google Scholar]
  17. Muffler K., Ulber R.. ( 2008;). Fed-batch cultivation of the marine bacterium Sulfitobacter pontiacus using immobilized substrate and purification of sulfite oxidase by application of membrane adsorber technology. . Biotechnol Bioeng 99:, 870–875. [CrossRef][PubMed]
    [Google Scholar]
  18. Park J. R., Bae J. W., Nam Y. D., Chang H. W., Kwon H. Y., Quan Z. X., Park Y. H.. ( 2007;). Sulfitobacter litoralis sp. nov., a marine bacterium isolated from the East Sea, Korea. . Int J Syst Evol Microbiol 57:, 692–695. [CrossRef][PubMed]
    [Google Scholar]
  19. Pukall R., Buntefuß D., Frühling A., Rohde M., Kroppenstedt R. M., Burghardt J., Lebaron P., Bernard L., Stackebrandt E.. ( 1999;). Sulfitobacter mediterraneus sp. nov., a new sulfite-oxidizing member of the α-Proteobacteria. . Int J Syst Bacteriol 49:, 513–519. [CrossRef][PubMed]
    [Google Scholar]
  20. Sasser M.. ( 1990;). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101 Newark. DE:: MIDI Inc;.
    [Google Scholar]
  21. Skerman V. B. D.. ( 1967;). A Guide to the Identification of the Genera of Bacteria, with Methods and Digests of Generic Characteristics, , 2nd edn.. Baltimore, MD:: Williams & Wilkins;.
    [Google Scholar]
  22. Sorokin D. Y.. ( 1995b;). Sulfitobacter pontiacus gen. nov, sp. nov - a new heterotrophic bacterium from the black-sea, specialized on sulfite oxidation. . Microbiology 64:, 295–305.
    [Google Scholar]
  23. Swofford D. L.. ( 1993;). paup: a computer-program for phylogenetic inference using maximum parsimony. . J Gen Physiol 102:, A9.
    [Google Scholar]
  24. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S.. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739. [CrossRef][PubMed]
    [Google Scholar]
  25. Yoon J. H., Kang S. J., Oh T. K.. ( 2007a;). Sulfitobacter marinus sp. nov., isolated from seawater of the East Sea in Korea. . Int J Syst Evol Microbiol 57:, 302–305. [CrossRef][PubMed]
    [Google Scholar]
  26. Yoon J-H., Kang S-J., Lee M-H., Oh T-K.. ( 2007b;). Description of Sulfitobacter donghicola sp. nov., isolated from seawater of the East Sea in Korea, transfer of Staleya guttiformis Labrenz et al. 2000 to the genus Sulfitobacter as Sulfitobacter guttiformis comb. nov. and emended description of the genus Sulfitobacter. . Int J Syst Evol Microbiol 57:, 1788–1792. [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.065961-0
Loading
/content/journal/ijsem/10.1099/ijs.0.065961-0
Loading

Data & Media loading...

Supplements

Supplementary Data 

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error