1887

Abstract

A novel actinobacterial strain, designated Z1R7, was isolated from a soil sample collected from Burgazada, in the Marmara Sea (Turkey), and the strain identity was determined using a polyphasic taxonomic approach. The organism had chemotaxonomic and morphological properties consistent with its classification in the genus and it formed a distinct phyletic line in the 16S rRNA gene tree, together with the type strains GW41-1564 (95.76 %), YIM 60475 (95.64 %), YIM 47672 (95.53 %), S1412 (95.29 %), MCCC 1A01535 (94.74 %), YIM 65188 (94.59 %) and NEAU-LZS-5 (94.68 %). Chomotaxonomic data revealed that strain Z1R7 possesed MK-9 (H) as the predominant menaquinone, -diaminopimelic acid as the diagnostic diamino acid, and galactose, glucose and ribose as whole cell sugars. Diphosphatidylglycerol, phoshphatidylethanolamine and phosphatidylinositol were the predominant polar lipids; iso-C, anteiso-C and anteiso-C were the major fatty acids, and the genomic DNA G+C content was 69.4 mol%. On the basis of these genotypic and phenotypic data, it is proposed that isolate Z1R7 ( = KCTC 29434 = DSM 42126) should be classified in the genus as sp. nov.

Funding
This study was supported by the:
  • , Ondokuz Mayis University , (Award PYO. FEN. 1904.13.004)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.065870-0
2014-12-01
2021-03-08
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/64/12/4043.html?itemId=/content/journal/ijsem/10.1099/ijs.0.065870-0&mimeType=html&fmt=ahah

References

  1. Bérdy J. ( 2005 ). Bioactive microbial metabolites. . J Antibiot 58, 126. [CrossRef] [PubMed]
    [Google Scholar]
  2. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E. ( 1977 ). Distribution of menaquinones in actinomycetes and corynebacteria. . J Gen Microbiol 100, 221230. [CrossRef] [PubMed]
    [Google Scholar]
  3. Felsenstein J. ( 1981 ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17, 368376. [CrossRef] [PubMed]
    [Google Scholar]
  4. Felsenstein J. ( 1985 ). Confidence limits on phylogeny: an approach using the bootstrap. . Evolution 39, 783791. [CrossRef]
    [Google Scholar]
  5. Gonzalez J. M., Saiz-Jimenez C. ( 2005 ). A simple fluorimetric method for the estimation of DNA-DNA relatedness between closely related microorganisms by thermal denaturation temperatures. . Extremophiles 9, 7579. [CrossRef] [PubMed]
    [Google Scholar]
  6. Goodfellow M., Fiedler H.-P. ( 2010 ). A guide to successful bioprospecting: informed by actinobacterial systematics. . Antonie van Leeuwenhoek 98, 119142. [CrossRef] [PubMed]
    [Google Scholar]
  7. Jones K. L. ( 1949 ). Fresh isolates of actinomycetes in which the presence of sporogenous aerial mycelia is a fluctuating characteristic. . J Bacteriol 57, 141145.[PubMed]
    [Google Scholar]
  8. Jukes T. H., Cantor C. R. ( 1969 ). Evolution of protein molecules. . In Mammalian Protein Metabolism, vol. 3, pp. 21132. Edited by Munro H. N. . New York:: Academic Press;. [CrossRef]
    [Google Scholar]
  9. Kämpfer P. ( 2012 ). Genus I. Streptomyces Waksman and Henrici 1943, 339 emend Witt and Stackebrandt 1990, 370 emend Wellington, Stackebrandt, Sanders, Wolstrup and Jorgensen 1992, 159. . In Bergey’s manual of systematic bacteriology, vol. 5, pp. 14551767. Edited by Whitman W., Goodfellow M., Kämpfer P., Busse H.-J., Trujillo M. E., Garrity G., Ludwig W., Suzuki K., Parte A. . New York, Dordrecht, Heidelberg, London:: Springer;.
    [Google Scholar]
  10. Kämpfer P., Kroppenstedt R. M. ( 1996 ). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. . Can J Microbiol 42, 9891005. [CrossRef]
    [Google Scholar]
  11. Kelly K. L. ( 1964 ). Inter-Society Color Council-National Bureau of Standards Color Name Charts Illustrated with Centroid Colors Washington, DC:: US Government Printing Office;.
    [Google Scholar]
  12. Kim O.-S., Cho Y.-J., Lee K., Yoon S.-H., Kim M., Na H., Park S.-C., Jeon Y. S., Lee J. H. & other authors ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716721. [CrossRef] [PubMed]
    [Google Scholar]
  13. Kluge A. G., Farris F. S. ( 1969 ). Quantitative phyletics and the evolution of anurans. . Syst Zool 18, 132. [CrossRef]
    [Google Scholar]
  14. Kroppenstedt R. M. ( 1982 ). Separation of bacterial menaquinones by HPLC using reverse phase (RP18) and a silver loaded ion exchanger as stationary phases. . J Liq Chromatogr 5, 23592367. [CrossRef]
    [Google Scholar]
  15. Kroppenstedt R. M., Goodfellow M. ( 2006 ). The family Thermomonosporaceae: Actinocorallia, Actinomadura, Spirillispora and Thermomonospora . . In The Prokaryotes, Archaea and Bacteria: Firmicutes, Actinomycetes, , 3rd edn., vol. 3, pp. 682724. Edited by Dworkin M., Falkow S., Schleifer K. H., Stackebrandt E. . New York:: Springer;. [CrossRef]
    [Google Scholar]
  16. Lechevalier M. P., Lechevalier H. ( 1970 ). Chemical composition as a criterion in the classification of aerobic actinomycetes. . Int J Syst Bacteriol 20, 435443. [CrossRef]
    [Google Scholar]
  17. Lechevalier M. P., Lechevalier H. A. ( 1980 ). The chemotaxonomy of actinomycetes. . In Actinomycete Taxonomy, pp. 227291. Edited by Dietz A., Thayer D. W. . Fairfax, VA:: Society for Industrial Microbiology;.
    [Google Scholar]
  18. Lechevalier M. P., De Bièvre C., Lechevalier H. A. ( 1977 ). Chemotaxonomy of aerobic actinomycetes: phospholipid composition. . Biochem Syst Ecol 5, 249260. [CrossRef]
    [Google Scholar]
  19. Manfio G. P., Atalan E., Zakrzewska-Czerwinska J., Mordarski M., Rodríguez C., Collins M. D., Goodfellow M. ( 2003 ). Classification of novel soil streptomycetes as Streptomyces aureus sp. nov., Streptomyces laceyi sp. nov. and Streptomyces sanglieri sp. nov.. Antonie van Leeuwenhoek 83, 245255. [CrossRef] [PubMed]
    [Google Scholar]
  20. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. ( 1984 ). An integrated producer for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2, 233241. [CrossRef]
    [Google Scholar]
  21. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  22. Santhanam R., Rong X., Huang Y., Goodfellow M. ( 2013 ). Streptomyces erringtonii sp. nov. and Streptomyces kaempferi sp. nov., isolated from a hay meadow soil. . Antonie van Leeuwenhoek 103, 7987. [CrossRef] [PubMed]
    [Google Scholar]
  23. Sasser M. ( 1990 ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. . Newark, DE:: MIDI Inc;.
  24. Shirling E. B., Gottlieb D. ( 1966 ). Methods for characterization of Streptomyces species. . Int J Syst Bacteriol 16, 313340. [CrossRef]
    [Google Scholar]
  25. Staneck J. L., Roberts G. D. ( 1974 ). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. . Appl Microbiol 28, 226231.[PubMed]
    [Google Scholar]
  26. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 27312739. [CrossRef] [PubMed]
    [Google Scholar]
  27. Veyisoglu A., Sahin N. ( 2014 ). Streptomyces hoynatensis sp. nov., isolated from deep marine sediment. . Int J Syst Evol Microbiol 64, 819826. [CrossRef] [PubMed]
    [Google Scholar]
  28. Waksman S. A. ( 1967 ). The Actinomycetes, A summary of current knowledge. . New York:: Ronald Press;.
  29. Waksman S. A., Henrici A. T. ( 1943 ). The nomenclature and classifcation of the actinomycetes. . J Bacteriol 46, 337341.[PubMed]
    [Google Scholar]
  30. Weisburg W. G., Barns S. M., Pelletier D. A., Lane D. J. ( 1991 ). 16S ribosomal DNA amplification for phylogenetic study. . J Bacteriol 173, 697703.[PubMed]
    [Google Scholar]
  31. Williams S. T., Goodfellow M., Alderson G., Wellington E. M. H., Sneath P. H. A., Sackin M. J. ( 1983 ). Numerical classification of Streptomyces and related genera. . J Gen Microbiol 129, 17431813.[PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.065870-0
Loading
/content/journal/ijsem/10.1099/ijs.0.065870-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error