1887

Abstract

A Gram-staining-positive, aerobic, motile and non-spore-forming actinobacteria, designated strain F10, was isolated from a deep-sea sediment of the western Pacific Ocean. Phylogenetic and phenotypic properties of the organism supported that it belonged to the genus . Strain F10 shared highest 16S rRNA gene sequence similarity of 96.8 % with DSM 17733, followed by YIM 70097 (96.7 %) and CAAS 252 (96.6 %). The organism grew at 4–50 °C, at pH 7.0–12.0 and in the presence of 0–12 % (w/v) NaCl, with optimal growth occurring at 40 °C, at pH 9.0 and in the presence of 1 % (w/v) NaCl. The peptidoglycan type was A4(alpha), -Lys–Gly–-Glu. The polar lipid profile of strain F10 consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, two unknown glycolipids and two unknown lipids. The isolate contained MK-9 (92 %) and MK-8 (5.8 %) as the major components of the menaquinone system, and anteiso-C (50.9 %) and anteiso-C (29.8 %) as the predominant fatty acids. The G+C content of the genomic DNA of strain F10 was 66.2 mol%. Based on phenotypic, genotypic and phylogenetic analyses, strain F10 represents a novel species of the genus for which the name sp. nov. is proposed. The type strain is F10 ( = LMG 28112 = CGMCC 1.12781 = JCM 19766 = MCCC 1A09946).

Funding
This study was supported by the:
  • COMRA program (Award DY125-15-R-01)
  • National Natural Science Foundation of China (Award 41206160 and 41006072)
  • National Infrastructure of Microbial Resources (Award NIRM2014-9)
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2015-02-01
2021-04-22
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References

  1. Cerny G. ( 1978 ). Studies on the aminopeptidase test for the distinction of gram-negative from gram-positive bacteria. . Eur J Appl Microbiol Biotechnol 5, 113122. [CrossRef]
    [Google Scholar]
  2. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E. ( 1977 ). Distribution of menaquinones in actinomycetes and corynebacteria. . J Gen Microbiol 100, 221230. [CrossRef] [PubMed]
    [Google Scholar]
  3. Collins M. D., Lawson P. A., Labrenz M., Tindall B. J., Weiss N., Hirsch P. ( 2002 ). Nesterenkonia lacusekhoensis sp. nov., isolated from hypersaline Ekho Lake, East Antarctica, and emended description of the genus Nesterenkonia . . Int J Syst Evol Microbiol 52, 11451150. [CrossRef] [PubMed]
    [Google Scholar]
  4. Delgado O., Quillaguamán J., Bakhtiar S., Mattiasson B., Gessesse A., Hatti-Kaul R. ( 2006 ). Nesterenkonia aethiopica sp. nov., an alkaliphilic, moderate halophile isolated from an Ethiopian soda lake. . Int J Syst Evol Microbiol 56, 12291232. [CrossRef] [PubMed]
    [Google Scholar]
  5. Felsenstein J. ( 1981 ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17, 368376. [CrossRef] [PubMed]
    [Google Scholar]
  6. Felsenstein J. ( 1985 ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39, 783791. [CrossRef]
    [Google Scholar]
  7. Fitch W. M. ( 1971 ). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20, 406416. [CrossRef]
    [Google Scholar]
  8. Govender L., Naidoo L., Setati M. E. ( 2013 ). Nesterenkonia suensis sp. nov., a haloalkaliphilic actinobacterium isolated from a salt pan. . Int J Syst Evol Microbiol 63, 4146. [CrossRef] [PubMed]
    [Google Scholar]
  9. Kates M. ( 1986 ). General analytical procedures. . In Techniques of Lipidology, pp. 112185. Edited by Kates M. . Amsterdam:: Elsevier;.
    [Google Scholar]
  10. Kim O.-S., Cho Y.-J., Lee K., Yoon S.-H., Kim M., Na H., Park S.-C., Jeon Y. S., Lee J.-H. & other authors ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716721. [CrossRef] [PubMed]
    [Google Scholar]
  11. Kimura M. ( 1980 ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16, 111120. [CrossRef] [PubMed]
    [Google Scholar]
  12. Leifson E. ( 1960 ). Atlas of Bacterial Flagellation. London:: Academic Press;.
    [Google Scholar]
  13. Li W. J., Chen H. H., Zhang Y. Q., Schumann P., Stackebrandt E., Xu L. H., Jiang C. L. ( 2004 ). Nesterenkonia halotolerans sp. nov. and Nesterenkonia xinjiangensis sp. nov., actinobacteria from saline soils in the west of China. . Int J Syst Evol Microbiol 54, 837841. [CrossRef] [PubMed]
    [Google Scholar]
  14. Li W. J., Chen H. H., Kim C. J., Zhang Y. Q., Park D. J., Lee J. C., Xu L. H., Jiang C. L. ( 2005 ). Nesterenkonia sandarakina sp. nov. and Nesterenkonia lutea sp. nov., novel actinobacteria, and emended description of the genus Nesterenkonia . . Int J Syst Evol Microbiol 55, 463466. [CrossRef] [PubMed]
    [Google Scholar]
  15. Li W. J., Xu P., Schumann P., Zhang Y. Q., Pukall R., Xu L. H., Stackebrandt E., Jiang C. L. ( 2007 ). Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China), and emended description of the genus Georgenia . . Int J Syst Evol Microbiol 57, 14241428. [CrossRef] [PubMed]
    [Google Scholar]
  16. Li W. J., Zhang Y. Q., Schumann P., Liu H. Y., Yu L. Y., Zhang Y. Q., Stackebrandt E., Xu L. H., Jiang C. L. ( 2008 ). Nesterenkonia halophila sp. nov., a moderately halophilic, alkalitolerant actinobacterium isolated from a saline soil. . Int J Syst Evol Microbiol 58, 13591363. [CrossRef] [PubMed]
    [Google Scholar]
  17. Luo H. Y., Miao L. H., Fang C., Yang P. L., Wang Y. R., Shi P. J., Yao B., Fan Y. L. ( 2008 ). Nesterenkonia flava sp. nov., isolated from paper-mill effluent. . Int J Syst Evol Microbiol 58, 19271930. [CrossRef] [PubMed]
    [Google Scholar]
  18. Luo H. Y., Wang Y. R., Miao L. H., Yang P. L., Shi P. J., Fang C. X., Yao B., Fan Y. L. ( 2009 ). Nesterenkonia alba sp. nov., an alkaliphilic actinobacterium isolated from the black liquor treatment system of a cotton pulp mill. . Int J Syst Evol Microbiol 59, 863868. [CrossRef] [PubMed]
    [Google Scholar]
  19. Mesbah M., Premachandran U., Whitman W. B. ( 1989 ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39, 159167. [CrossRef]
    [Google Scholar]
  20. Mota R. R., Márquez M. C., Arahal D. R., Mellado E., Ventosa A. ( 1997 ). Polyphasic taxonomy of Nesterenkonia halobia . . Int J Syst Bacteriol 47, 12311235. [CrossRef] [PubMed]
    [Google Scholar]
  21. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  22. Sasser M. ( 1990 ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. . Newark, DE:: MIDI Inc;.
  23. Schleifer K. H. ( 1985 ). Analysis of the chemical composition and primary structure of murein. . Methods Microbiol 18, 123156. [CrossRef]
    [Google Scholar]
  24. Schleifer K. H., Kandler O. ( 1972 ). Peptidoglycan types of bacterial cell walls and their taxonomic implications. . Bacteriol Rev 36, 407477.[PubMed]
    [Google Scholar]
  25. Schumann P. ( 2011 ). Peptidoglycan Structure. . Methods Microbiol 38, 101129. [CrossRef]
    [Google Scholar]
  26. Stackebrandt E., Goebel B. M. ( 1994 ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol 44, 846849. [CrossRef]
    [Google Scholar]
  27. Stackebrandt E., Koch C., Gvozdiak O., Schumann P. ( 1995 ). Taxonomic dissection of the genus Micrococcus: Kocuria gen. nov., Nesterenkonia gen. nov., Kytococcus gen. nov., Dermacoccus gen. nov., and Micrococcus Cohn 1872 gen. emend.. Int J Syst Bacteriol 45, 682692. [CrossRef] [PubMed]
    [Google Scholar]
  28. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 27312739. [CrossRef] [PubMed]
    [Google Scholar]
  29. Xu L. H., Li W. J., Liu Z. H., Jiang C. L. ( 2007 ). Physiological and biochemical characteristics. . In Actinomycete Systematics: Principles, Methods and Practice. pp. 40–53. Beijing:: Science Press;.
    [Google Scholar]
  30. Yoon J. H., Jung S. Y., Kim W., Nam S. W., Oh T. K. ( 2006 ). Nesterenkonia jeotgali sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. . Int J Syst Evol Microbiol 56, 25872592. [CrossRef] [PubMed]
    [Google Scholar]
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