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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 65, Issue Pt_2

sp. nov., an alkaliphilic, halotolerant actinobacteria isolated from the western Pacific Ocean Free

Abstract

A Gram-staining-positive, aerobic, motile and non-spore-forming actinobacteria, designated strain F10, was isolated from a deep-sea sediment of the western Pacific Ocean. Phylogenetic and phenotypic properties of the organism supported that it belonged to the genus . Strain F10 shared highest 16S rRNA gene sequence similarity of 96.8 % with DSM 17733, followed by YIM 70097 (96.7 %) and CAAS 252 (96.6 %). The organism grew at 4–50 °C, at pH 7.0–12.0 and in the presence of 0–12 % (w/v) NaCl, with optimal growth occurring at 40 °C, at pH 9.0 and in the presence of 1 % (w/v) NaCl. The peptidoglycan type was A4(alpha), -Lys–Gly–-Glu. The polar lipid profile of strain F10 consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, two unknown glycolipids and two unknown lipids. The isolate contained MK-9 (92 %) and MK-8 (5.8 %) as the major components of the menaquinone system, and anteiso-C (50.9 %) and anteiso-C (29.8 %) as the predominant fatty acids. The G+C content of the genomic DNA of strain F10 was 66.2 mol%. Based on phenotypic, genotypic and phylogenetic analyses, strain F10 represents a novel species of the genus for which the name sp. nov. is proposed. The type strain is F10 ( = LMG 28112 = CGMCC 1.12781 = JCM 19766 = MCCC 1A09946).

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Funding
This study was supported by the:
  • COMRA program (Award DY125-15-R-01)
  • National Natural Science Foundation of China (Award 41206160 and 41006072)
  • National Infrastructure of Microbial Resources (Award NIRM2014-9)
© 2015 IUMS
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2015-02-01
2025-04-23
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Nesterenkonia alkaliphila sp. nov., an alkaliphilic, halotolerant actinobacteria isolated from the western Pacific Ocean
Int J Syst Evol Microbiol 65, 516 (2015); https://doi.org/10.1099/ijs.0.065623-0
/content/journal/ijsem/10.1099/ijs.0.065623-0
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