1887

Abstract

A novel actinobacterium, designated strain NEAU-yn17, was isolated from a soil sample collected at the wastewater discharge site of a pesticide factory in Harbin, northern China, and characterized using a polyphasic approach. Morphological and chemotaxonomic properties of strain NEAU-yn17 were consistent with the description of the genus , such as the spore arrangement, the diamino acid of the peptidoglycan, the whole-cell hydrolysates, the predominant menaquinone and the phospholipid profile. Phylogenetic analysis based on 16S rRNA gene sequences demonstrated that strain NEAU-yn17 should also be classified in the genus , with DSM 45456 (99.52 % sequence similarity) and JCM 12329 (99.04 %) as the nearest phylogenetic relatives. A combination of DNA–DNA hybridization results and some phenotypic characteristics indicated that strain NEAU-yn17 can be distinguished from its closest relatives. Therefore, strain NEAU-yn17 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is NEAU-yn17 ( = CGMCC 4.7097 = DSM 45878).

Funding
This study was supported by the:
  • , National Outstanding Youth Foundation , (Award 31225024)
  • , National Key Project for Basic Research , (Award 2010CB126102)
  • , National Key Technology R&D Program , (Award 2012BAD19B06)
  • , Program for New Century Excellent Talents in University , (Award NCET-11-0953)
  • , National Natural Science Foundation of China , (Award 31071750, 31171913 and 31372006)
  • , Outstanding Youth Foundation of Heilongjiang Province , (Award JC201201)
  • , Chang Jiang Scholar Candidates Program for Provincial Universities in Heilongjiang (CSCP)
  • , Science and Technology Research Project of Heilongjiang Provincial Educational Commission , (Award 12541001)
  • , Youth Science Foundation of Heilongjiang Province , (Award QC2014C013)
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2014-12-01
2020-09-25
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References

  1. Atlas R. M. ( 1993 ). Handbook of Microbiological Media. Edited by Parks L. C. . Boca Raton, FL:: CRC Press;.
    [Google Scholar]
  2. Boubetra D., Zitouni A., Bouras N., Mathieu F., Lebrihi A., Schumann P., Spröer C., Klenk H. P., Sabaou N. ( 2013a ). Saccharothrix hoggarensis sp. nov., an actinomycete isolated from Saharan soil. . Int J Syst Evol Microbiol 63, 549553. [CrossRef] [PubMed]
    [Google Scholar]
  3. Boubetra D., Zitouni A., Bouras N., Mathieu F., Lebrihi A., Schumann P., Spröer C., Klenk H. P., Sabaou N. ( 2013b ). Saccharothrix saharensis sp. nov., an actinomycete isolated from Algerian Saharan soil. . Int J Syst Evol Microbiol 63, 37443749. [CrossRef] [PubMed]
    [Google Scholar]
  4. Collins M. D. ( 1985 ). Isoprenoid quinone analysis in classification and identification. . In Chemical Methods in Bacterial Systematics, pp. 267287. Edited by Goodfellow M., Minnikin D. E. . London:: Academic Press;.
    [Google Scholar]
  5. De Ley J., Cattoir H., Reynaerts A. ( 1970 ). The quantitative measurement of DNA hybridization from renaturation rates. . Eur J Biochem 12, 133142. [CrossRef] [PubMed]
    [Google Scholar]
  6. Felsenstein J. ( 1981 ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17, 368376. [CrossRef] [PubMed]
    [Google Scholar]
  7. Felsenstein J. ( 1985 ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39, 783791. [CrossRef]
    [Google Scholar]
  8. Gao R. X., Liu C. X., Zhao J. W., Jia F. Y., Yu C., Yang L. Y., Wang X. J., Xiang W. S. ( 2014 ). Micromonospora jinlongensis sp. nov., isolated from muddy soil in China and emended description of the genus Micromonospora . . Antonie van Leeuwenhoek 105, 307315. [CrossRef] [PubMed]
    [Google Scholar]
  9. Gordon R. E., Barnett D. A., Handerhan J. E., Pang C. ( 1974 ). Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. . Int J Syst Bacteriol 24, 5463. [CrossRef]
    [Google Scholar]
  10. Hayakawa M., Nonomura H. ( 1987 ). Humic acid-vitamin agar, a new medium for the selective isolation of soil actinomycetes. . J Ferment Technol 65, 501509. [CrossRef]
    [Google Scholar]
  11. Huss V. A. R., Festl H., Schleifer K. H. ( 1983 ). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. . Syst Appl Microbiol 4, 184192. [CrossRef] [PubMed]
    [Google Scholar]
  12. Kelly K. L. ( 1964 ). Inter-Society Color Council – National Bureau of Standards Color Name Charts Illustrated with Centroid Colors. Washington, DC:: US Government Printing Office;.
    [Google Scholar]
  13. Kim S. B., Brown R., Oldfield C., Gilbert S. C., Iliarionov S., Goodfellow M. ( 2000 ). Gordonia amicalis sp. nov., a novel dibenzothiophene-desulphurizing actinomycete. . Int J Syst Evol Microbiol 50, 20312036. [CrossRef] [PubMed]
    [Google Scholar]
  14. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. & other authors ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716721. [CrossRef] [PubMed]
    [Google Scholar]
  15. Kimura M. ( 1980 ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16, 111120. [CrossRef] [PubMed]
    [Google Scholar]
  16. Labeda D. P., Kroppenstedt R. M. ( 2000 ). Phylogenetic analysis of Saccharothrix and related taxa: proposal for Actinosynnemataceae fam. nov.. Int J Syst Evol Microbiol 50, 331336. [CrossRef] [PubMed]
    [Google Scholar]
  17. Labeda D. P., Lechevalier M. P. ( 1989 ). Amendment of the genus Saccharothrix Labeda et al. 1984 and descriptions of Saccharothrix espanaensis sp. nov., Saccharothrix cryophilis sp. nov., and Saccharothrix mutabilis comb. nov.. Int J Syst Bacteriol 39, 420423. [CrossRef]
    [Google Scholar]
  18. Labeda D. P., Testa R. T., Lechevalier M. P., Lechevalier H. A. ( 1984 ). Saccharothrix: a new genus of the Actinomycetales related to Nocardiopsis . . Int J Syst Bacteriol 34, 426431. [CrossRef]
    [Google Scholar]
  19. Lechevalier M. P., Lechevalier H. A. ( 1980 ). The chemotaxonomy of actinomycetes. . In Actinomycete Taxonomy (Society for Industrial Microbiology Special Publication no. 6), pp. 227291. Edited by Dietz A., Thayer D. W. . Arlington, VA:: Society for Industrial Microbiology;.
    [Google Scholar]
  20. Mandel M., Marmur J. ( 1968 ). Use of ultraviolet absorbance temperature profile for determining the guanine plus cytosine content of DNA. . Methods Enzymol 12B, 195206. [CrossRef]
    [Google Scholar]
  21. McKerrow J., Vagg S., McKinney T., Seviour E. M., Maszenan A. M., Brooks P., Seviour R. J. ( 2000 ). A simple HPLC method for analysing diaminopimelic acid diastereomers in cell walls of Gram-positive bacteria. . Lett Appl Microbiol 30, 178182. [CrossRef] [PubMed]
    [Google Scholar]
  22. Minnikin D. E., Hutchinson I. G., Caldicott A. B., Goodfellow M. ( 1980 ). Thin-layer chromatography of methanolysates of mycolic acid-containing bacteria. . J Chromatogr A 188, 221233. [CrossRef]
    [Google Scholar]
  23. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. ( 1984 ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2, 233241. [CrossRef]
    [Google Scholar]
  24. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  25. Shirling E. B., Gottlieb D. ( 1966 ). Methods for characterization of Streptomyces species. . Int J Syst Bacteriol 16, 313340. [CrossRef]
    [Google Scholar]
  26. Smibert R. M., Krieg N. R. ( 1994 ). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  27. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 27312739. [CrossRef] [PubMed]
    [Google Scholar]
  28. Wang X. J., Zhao J. W., Liu C. X., Wang J. D., Shen Y., Jia F. Y., Wang L., Zhang J., Yu C., Xiang W. S. ( 2013 ). Nonomuraea solani sp. nov., an actinomycete isolated from eggplant root (Solanum melongena L.). . Int J Syst Evol Microbiol 63, 24182423. [CrossRef] [PubMed]
    [Google Scholar]
  29. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. & other authors ( 1987 ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37, 463464. [CrossRef]
    [Google Scholar]
  30. Wu C., Lu X., Qin M., Wang Y., Ruan J. ( 1989 ). Analysis of menaquinone compound in microbial cells by HPLC. . Microbiology [English translation of Microbiology (Beijing)] 16, 176178. [CrossRef]
    [Google Scholar]
  31. Xiang W. S., Liu C. X., Wang X. J., Du J., Xi L. J., Huang Y. ( 2011 ). Actinoalloteichus nanshanensis sp. nov., isolated from the rhizosphere of a fig tree (Ficus religiosa). . Int J Syst Evol Microbiol 61, 11651169. [CrossRef] [PubMed]
    [Google Scholar]
  32. Yokota A., Tamura T., Hasegawa T., Huang L. H. ( 1993 ). Catenuloplanes japonicus gen. nov., sp. nov., nom. rev., a new genus of the order Actinomycetales . . Int J Syst Bacteriol 43, 805812. [CrossRef]
    [Google Scholar]
  33. Zitouni A., Lamari L., Boudjella H., Badji B., Sabaou N., Gaouar A., Mathieu F., Lebrihi A., Labeda D. P. ( 2004 ). Saccharothrix algeriensis sp. nov., isolated from Saharan soil. . Int J Syst Evol Microbiol 54, 13771381. [CrossRef] [PubMed]
    [Google Scholar]
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