1887

Abstract

A novel actinobacterium, designated strain NEAU-yn17, was isolated from a soil sample collected at the wastewater discharge site of a pesticide factory in Harbin, northern China, and characterized using a polyphasic approach. Morphological and chemotaxonomic properties of strain NEAU-yn17 were consistent with the description of the genus , such as the spore arrangement, the diamino acid of the peptidoglycan, the whole-cell hydrolysates, the predominant menaquinone and the phospholipid profile. Phylogenetic analysis based on 16S rRNA gene sequences demonstrated that strain NEAU-yn17 should also be classified in the genus , with DSM 45456 (99.52 % sequence similarity) and JCM 12329 (99.04 %) as the nearest phylogenetic relatives. A combination of DNA–DNA hybridization results and some phenotypic characteristics indicated that strain NEAU-yn17 can be distinguished from its closest relatives. Therefore, strain NEAU-yn17 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is NEAU-yn17 ( = CGMCC 4.7097 = DSM 45878).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.065557-0
2014-12-01
2019-11-13
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/64/12/4033.html?itemId=/content/journal/ijsem/10.1099/ijs.0.065557-0&mimeType=html&fmt=ahah

References

  1. Atlas R. M.. ( 1993;). Handbook of Microbiological Media. Edited by Parks L. C... Boca Raton, FL:: CRC Press;.
    [Google Scholar]
  2. Boubetra D., Zitouni A., Bouras N., Mathieu F., Lebrihi A., Schumann P., Spröer C., Klenk H. P., Sabaou N.. ( 2013a;). Saccharothrix hoggarensis sp. nov., an actinomycete isolated from Saharan soil. . Int J Syst Evol Microbiol 63:, 549–553. [CrossRef][PubMed]
    [Google Scholar]
  3. Boubetra D., Zitouni A., Bouras N., Mathieu F., Lebrihi A., Schumann P., Spröer C., Klenk H. P., Sabaou N.. ( 2013b;). Saccharothrix saharensis sp. nov., an actinomycete isolated from Algerian Saharan soil. . Int J Syst Evol Microbiol 63:, 3744–3749. [CrossRef][PubMed]
    [Google Scholar]
  4. Collins M. D.. ( 1985;). Isoprenoid quinone analysis in classification and identification. . In Chemical Methods in Bacterial Systematics, pp. 267–287. Edited by Goodfellow M., Minnikin D. E... London:: Academic Press;.
    [Google Scholar]
  5. De Ley J., Cattoir H., Reynaerts A.. ( 1970;). The quantitative measurement of DNA hybridization from renaturation rates. . Eur J Biochem 12:, 133–142. [CrossRef][PubMed]
    [Google Scholar]
  6. Felsenstein J.. ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17:, 368–376. [CrossRef][PubMed]
    [Google Scholar]
  7. Felsenstein J.. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  8. Gao R. X., Liu C. X., Zhao J. W., Jia F. Y., Yu C., Yang L. Y., Wang X. J., Xiang W. S.. ( 2014;). Micromonospora jinlongensis sp. nov., isolated from muddy soil in China and emended description of the genus Micromonospora. . Antonie van Leeuwenhoek 105:, 307–315. [CrossRef][PubMed]
    [Google Scholar]
  9. Gordon R. E., Barnett D. A., Handerhan J. E., Pang C.. ( 1974;). Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. . Int J Syst Bacteriol 24:, 54–63. [CrossRef]
    [Google Scholar]
  10. Hayakawa M., Nonomura H.. ( 1987;). Humic acid-vitamin agar, a new medium for the selective isolation of soil actinomycetes. . J Ferment Technol 65:, 501–509. [CrossRef]
    [Google Scholar]
  11. Huss V. A. R., Festl H., Schleifer K. H.. ( 1983;). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. . Syst Appl Microbiol 4:, 184–192. [CrossRef][PubMed]
    [Google Scholar]
  12. Kelly K. L.. ( 1964;). Inter-Society Color Council – National Bureau of Standards Color Name Charts Illustrated with Centroid Colors. Washington, DC:: US Government Printing Office;.
    [Google Scholar]
  13. Kim S. B., Brown R., Oldfield C., Gilbert S. C., Iliarionov S., Goodfellow M.. ( 2000;). Gordonia amicalis sp. nov., a novel dibenzothiophene-desulphurizing actinomycete. . Int J Syst Evol Microbiol 50:, 2031–2036. [CrossRef][PubMed]
    [Google Scholar]
  14. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H.. & other authors ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62:, 716–721. [CrossRef][PubMed]
    [Google Scholar]
  15. Kimura M.. ( 1980;). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16:, 111–120. [CrossRef][PubMed]
    [Google Scholar]
  16. Labeda D. P., Kroppenstedt R. M.. ( 2000;). Phylogenetic analysis of Saccharothrix and related taxa: proposal for Actinosynnemataceae fam. nov.. Int J Syst Evol Microbiol 50:, 331–336. [CrossRef][PubMed]
    [Google Scholar]
  17. Labeda D. P., Lechevalier M. P.. ( 1989;). Amendment of the genus Saccharothrix Labeda et al. 1984 and descriptions of Saccharothrix espanaensis sp. nov., Saccharothrix cryophilis sp. nov., and Saccharothrix mutabilis comb. nov.. Int J Syst Bacteriol 39:, 420–423. [CrossRef]
    [Google Scholar]
  18. Labeda D. P., Testa R. T., Lechevalier M. P., Lechevalier H. A.. ( 1984;). Saccharothrix: a new genus of the Actinomycetales related to Nocardiopsis. . Int J Syst Bacteriol 34:, 426–431. [CrossRef]
    [Google Scholar]
  19. Lechevalier M. P., Lechevalier H. A.. ( 1980;). The chemotaxonomy of actinomycetes. . In Actinomycete Taxonomy (Society for Industrial Microbiology Special Publication no. 6), pp. 227–291. Edited by Dietz A., Thayer D. W... Arlington, VA:: Society for Industrial Microbiology;.
    [Google Scholar]
  20. Mandel M., Marmur J.. ( 1968;). Use of ultraviolet absorbance temperature profile for determining the guanine plus cytosine content of DNA. . Methods Enzymol 12B:, 195–206. [CrossRef]
    [Google Scholar]
  21. McKerrow J., Vagg S., McKinney T., Seviour E. M., Maszenan A. M., Brooks P., Seviour R. J.. ( 2000;). A simple HPLC method for analysing diaminopimelic acid diastereomers in cell walls of Gram-positive bacteria. . Lett Appl Microbiol 30:, 178–182. [CrossRef][PubMed]
    [Google Scholar]
  22. Minnikin D. E., Hutchinson I. G., Caldicott A. B., Goodfellow M.. ( 1980;). Thin-layer chromatography of methanolysates of mycolic acid-containing bacteria. . J Chromatogr A 188:, 221–233. [CrossRef]
    [Google Scholar]
  23. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H.. ( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2:, 233–241. [CrossRef]
    [Google Scholar]
  24. Saitou N., Nei M.. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  25. Shirling E. B., Gottlieb D.. ( 1966;). Methods for characterization of Streptomyces species. . Int J Syst Bacteriol 16:, 313–340. [CrossRef]
    [Google Scholar]
  26. Smibert R. M., Krieg N. R.. ( 1994;). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R... Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  27. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S.. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739. [CrossRef][PubMed]
    [Google Scholar]
  28. Wang X. J., Zhao J. W., Liu C. X., Wang J. D., Shen Y., Jia F. Y., Wang L., Zhang J., Yu C., Xiang W. S.. ( 2013;). Nonomuraea solani sp. nov., an actinomycete isolated from eggplant root (Solanum melongena L.). . Int J Syst Evol Microbiol 63:, 2418–2423. [CrossRef][PubMed]
    [Google Scholar]
  29. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E.. & other authors ( 1987;). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37:, 463–464. [CrossRef]
    [Google Scholar]
  30. Wu C., Lu X., Qin M., Wang Y., Ruan J.. ( 1989;). Analysis of menaquinone compound in microbial cells by HPLC. . Microbiology [English translation of Microbiology (Beijing)] 16:, 176–178. [CrossRef]
    [Google Scholar]
  31. Xiang W. S., Liu C. X., Wang X. J., Du J., Xi L. J., Huang Y.. ( 2011;). Actinoalloteichus nanshanensis sp. nov., isolated from the rhizosphere of a fig tree (Ficus religiosa). . Int J Syst Evol Microbiol 61:, 1165–1169. [CrossRef][PubMed]
    [Google Scholar]
  32. Yokota A., Tamura T., Hasegawa T., Huang L. H.. ( 1993;). Catenuloplanes japonicus gen. nov., sp. nov., nom. rev., a new genus of the order Actinomycetales. . Int J Syst Bacteriol 43:, 805–812. [CrossRef]
    [Google Scholar]
  33. Zitouni A., Lamari L., Boudjella H., Badji B., Sabaou N., Gaouar A., Mathieu F., Lebrihi A., Labeda D. P.. ( 2004;). Saccharothrix algeriensis sp. nov., isolated from Saharan soil. . Int J Syst Evol Microbiol 54:, 1377–1381. [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.065557-0
Loading
/content/journal/ijsem/10.1099/ijs.0.065557-0
Loading

Data & Media loading...

Supplements

Supplementary Data 

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error