1887

Abstract

A Gram-staining-negative bacterium, strain DSG-S4-2, was isolated from Dasugan Lake, a saline lake (salinity 3.1 %, w/v) in Qaidam basin, Qinghai, China and its taxonomic position was determined by using a polyphasic approach. Cells of strain DSG-S4-2 were non-spore-forming rods, 0.5–0.8 µm wide and 1.2–3.8 µm long and motile by means of a single polar flagellum. Strain DSG-S4-2 was strictly heterotrophic and aerobic, catalase-positive and oxidase-negative. and genes were present, bacteriochlorophyll (BChl ) and a carotenoid pigment were produced. Growth was observed in the presence of 0–8.0 % (w/v) NaCl (optimum, 1.0–2.0 %), at 20–40 °C (optimum, 35 °C) and pH 6.5–10.5 (optimum, pH 7.5–8.0). Strain DSG-S4-2 contained Q-10 as the sole respiratory quinone. The polar lipids contained two aminolipids, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, sulphoquinovosyldiacylglyceride, phosphatidylcholine and some unknown phospholipids, like the other members of the genus . The predominant fatty acid (>70 %) was summed feature 8 (Cω7 and/or Cω6). The DNA G+C content was 61.4 mol% (determined from melting temperature). Phylogenetic trees (neighbour-joining, maximum-likelihood and maximum-parsimony) based on 16S rRNA gene sequences showed that strain DSG-S4-2 was associated with the members of the genus , with highest 16S rRNA gene sequence similarity to OCh 254 (96.3 %) and OCh 368 (96.3 %). Based on the data presented above, it is concluded that strain DSG-S4-2 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is DSG-S4-2 ( = CGMCC 1.12426 = JCM 19310).

Funding
This study was supported by the:
  • , Agriculture-Transfer Foundation of China , (Award SQ2011EC3320022)
  • , Project of China State Key Laboratory of Simulation and Regulation of Water Cycle in River Basin , (Award 2013ZY06)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.065508-0
2014-08-01
2020-11-24
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/64/8/2812.html?itemId=/content/journal/ijsem/10.1099/ijs.0.065508-0&mimeType=html&fmt=ahah

References

  1. Achenbach L. A., Carey J., Madigan M. T. ( 2001 ). Photosynthetic and phylogenetic primers for detection of anoxygenic phototrophs in natural environments. . Appl Environ Microbiol 67, 29222926. [CrossRef] [PubMed]
    [Google Scholar]
  2. Allgaier M., Uphoff H., Felske A., Wagner-Döbler I. ( 2003 ). Aerobic anoxygenic photosynthesis in Roseobacter clade bacteria from diverse marine habitats. . Appl Environ Microbiol 69, 50515059. [CrossRef] [PubMed]
    [Google Scholar]
  3. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J. ( 1990 ). Basic local alignment search tool. . J Mol Biol 215, 403410. [CrossRef] [PubMed]
    [Google Scholar]
  4. Bibi F., Jeong J. H., Chung E. J., Jeon C. O., Chung Y. R. ( 2014 ). Labrenzia suaedae sp. nov., a marine bacterium isolated from a halophyte, and emended description of the genus Labrenzia . . Int J Syst Evol Microbiol 64, 11161122. [CrossRef] [PubMed]
    [Google Scholar]
  5. Biebl H., Wagner-Döbler I. ( 2006 ). Growth and bacteriochlorophyll a formation in taxonomically diverse aerobic anoxygenic phototrophic bacteria in chemostat culture: Influence of light regimen and starvation. . Process Biochem 41, 21532159. [CrossRef]
    [Google Scholar]
  6. Biebl H., Pukall R., Lünsdorf H., Schulz S., Allgaier M., Tindall B. J., Wagner-Döbler I. ( 2007 ). Description of Labrenzia alexandrii gen. nov., sp. nov., a novel alphaproteobacterium containing bacteriochlorophyll a, and a proposal for reclassification of Stappia aggregata as Labrenzia aggregata comb. nov., of Stappia marina as Labrenzia marina comb. nov. and of Stappia alba as Labrenzia alba comb. nov., and emended descriptions of the genera Pannonibacter, Stappia and Roseibium, and of the species Roseibium denhamense and Roseibium hamelinense . . Int J Syst Evol Microbiol 57, 10951107. [CrossRef] [PubMed]
    [Google Scholar]
  7. Chun J., Lee J. H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y. W. ( 2007 ). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. . Int J Syst Evol Microbiol 57, 22592261. [CrossRef] [PubMed]
    [Google Scholar]
  8. Collins M. D. ( 1985 ). Isoprenoid quinone analysis in classification and identification. . In Chemical Methods in Bacterial Systematics, pp. 267287. Edited by Goodfellow M., Minnikin D. E. . London:: Academic Press;.
    [Google Scholar]
  9. Dong X. Z., Cai M. Y. ( 2001 ). Determinative Manual for Routine Bacteriology. Beijing:: Scientific Press (English translation);.
    [Google Scholar]
  10. Felsenstein J. ( 1981 ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17, 368376. [CrossRef] [PubMed]
    [Google Scholar]
  11. Fitch W. M. ( 1971 ). Toward defining course of evolution-minimum change for a specific tree topology. . Syst Zool 20, 406416. [CrossRef]
    [Google Scholar]
  12. Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. (editors) ( 1994 ). Methods for General and Molecular Bacteriology. Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  13. Kates M. ( 1986 ). Techniques of Lipidology, , 2nd edn.. Amsterdam:: Elsevier;.
    [Google Scholar]
  14. Kim B. C., Park J. R., Bae J. W., Rhee S. K., Kim K. H., Oh J. W., Park Y. H. ( 2006 ). Stappia marina sp. nov., a marine bacterium isolated from the Yellow Sea. . Int J Syst Evol Microbiol 56, 7579. [CrossRef] [PubMed]
    [Google Scholar]
  15. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. & other authors ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716721. [CrossRef] [PubMed]
    [Google Scholar]
  16. Kimura M. ( 1983 ). The Neutral Theory of Molecular Evolution. Cambridge:: Cambridge University Press;. [CrossRef]
    [Google Scholar]
  17. King G. M. ( 2003 ). Molecular and culture-based analyses of aerobic carbon monoxide oxidizer diversity. . Appl Environ Microbiol 69, 72577265. [CrossRef] [PubMed]
    [Google Scholar]
  18. Labrenz M., Collins M. D., Lawson P. A., Tindall B. J., Schumann P., Hirsch P. ( 1999 ). Roseovarius tolerans gen. nov., sp. nov., a budding bacterium with variable bacteriochlorophyll a production from hypersaline Ekho Lake. . Int J Syst Bacteriol 49, 137147. [CrossRef] [PubMed]
    [Google Scholar]
  19. Marmur J., Doty P. ( 1962 ). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. . J Mol Biol 5, 109118. [CrossRef] [PubMed]
    [Google Scholar]
  20. Nokhal T. H., Schlegel H. G. ( 1983 ). Taxonomic study of Paracoccus denitrificans . . Int J Syst Bacteriol 33, 2637. [CrossRef]
    [Google Scholar]
  21. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  22. Suzuki T., Muroga Y., Takahama M., Nishimura Y. ( 2000 ). Roseigium denhamense gen. nov., sp. nov. and Roseibium hemelinense sp. nov., aerobic bacteriochlorophyll-containing bacteria isolated from the east and west coasts of Australia. . Int J Syst Evol Microbiol 50, 21512156. [CrossRef] [PubMed]
    [Google Scholar]
  23. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 27312739. [CrossRef] [PubMed]
    [Google Scholar]
  24. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. ( 1997 ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25, 48764882. [CrossRef] [PubMed]
    [Google Scholar]
  25. Weisburg W. G., Barns S. M., Pelletier D. A., Lane D. J. ( 1991 ). 16S ribosomal DNA amplification for phylogenetic study. . J Bacteriol 173, 697703.[PubMed]
    [Google Scholar]
  26. Wu C., Lu X., Qin M., Wang Y., Ruan J. ( 1989 ). Analysis of menaquinone compound in microbial cells by HPLC.. Microbiology [English translation of Microbiology (Beijing)] 16, 176178.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.065508-0
Loading
/content/journal/ijsem/10.1099/ijs.0.065508-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error