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Volume 64, Issue Pt_8

sp. nov., isolated from a saline lake Free

Abstract

A Gram-staining-negative bacterium, strain DSG-S4-2, was isolated from Dasugan Lake, a saline lake (salinity 3.1 %, w/v) in Qaidam basin, Qinghai, China and its taxonomic position was determined by using a polyphasic approach. Cells of strain DSG-S4-2 were non-spore-forming rods, 0.5–0.8 µm wide and 1.2–3.8 µm long and motile by means of a single polar flagellum. Strain DSG-S4-2 was strictly heterotrophic and aerobic, catalase-positive and oxidase-negative. and genes were present, bacteriochlorophyll (BChl ) and a carotenoid pigment were produced. Growth was observed in the presence of 0–8.0 % (w/v) NaCl (optimum, 1.0–2.0 %), at 20–40 °C (optimum, 35 °C) and pH 6.5–10.5 (optimum, pH 7.5–8.0). Strain DSG-S4-2 contained Q-10 as the sole respiratory quinone. The polar lipids contained two aminolipids, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, sulphoquinovosyldiacylglyceride, phosphatidylcholine and some unknown phospholipids, like the other members of the genus . The predominant fatty acid (>70 %) was summed feature 8 (Cω7 and/or Cω6). The DNA G+C content was 61.4 mol% (determined from melting temperature). Phylogenetic trees (neighbour-joining, maximum-likelihood and maximum-parsimony) based on 16S rRNA gene sequences showed that strain DSG-S4-2 was associated with the members of the genus , with highest 16S rRNA gene sequence similarity to OCh 254 (96.3 %) and OCh 368 (96.3 %). Based on the data presented above, it is concluded that strain DSG-S4-2 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is DSG-S4-2 ( = CGMCC 1.12426 = JCM 19310).

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Funding
This study was supported by the:
  • Agriculture-Transfer Foundation of China (Award SQ2011EC3320022)
  • Project of China State Key Laboratory of Simulation and Regulation of Water Cycle in River Basin (Award 2013ZY06)
© 2014 IUMS
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Roseibiumaquae sp. nov., isolated from a saline lake
Int J Syst Evol Microbiol 64, 2812 (2014); https://doi.org/10.1099/ijs.0.065508-0
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