1887

Abstract

Two Gram-negative, aerobic and rod-shaped or ovoid bacterial strains with different colony colours (greyish yellow and moderate orange), designated J-MR2-Y and J-MR2-O, were isolated from a tidal flat in the South Sea of South Korea. The two novel strains grew optimally at 35–37 °C. Strains J-MR2-Y and J-MR2-O showed no difference in their 16S rRNA gene sequences, and the mean DNA–DNA relatedness between them was 94 %. Phylogenetic trees based on 16S rRNA gene sequences revealed that strains J-MR2-Y and J-MR2-O clustered consistently with the type strains of , and , with which it exhibited 97.83–99.06 % sequence similarity. Sequence similarities to the type strains of the other recognized species of the genus were 94.01–96.26 %. Both strains contained Q-10 as the predominant ubiquinone, Cω7 as the major fatty acid and phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, one unidentified glycolipid and one unidentified aminolipid as the major polar lipids. The DNA G+C contents of strains J-MR2-Y and J-MR2-O were 68.1 and 68.4 mol%, respectively, and DNA–DNA relatedness values with the type strains of , and were 19–37 %. Differential phenotypic properties, together with their phylogenetic and genetic distinctiveness, revealed that the two novel strains are separated from other species of the genus . On the basis of the data presented, strains J-MR2-Y and J-MR2-O are proposed to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is J-MR2-Y ( = KCTC 42074 = CECT 8572).

Funding
This study was supported by the:
  • , National Institute of Biological Resources (NIBR) under the Ministry of Environment (MOE) and the Program for Collection, Management and Utilization of Biological Resources , (Award NRF-2013M3A9A5075953)
  • , Ministry of Science, ICT & Future Planning (MSIP) of the Republic of Korea
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2014-08-01
2020-07-10
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