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Volume 64, Issue Pt_11

gen. nov., sp. nov., a moderately halophilic bacterium from a hypersaline lake, and reclassification of as comb. nov. and as comb. nov. Free

Abstract

A novel Gram-stain-positive, moderately halophilic bacterium, designated strain B6B, was isolated from the water of an Iranian hypersaline lake, Aran-Bidgol, and characterized taxonomically using a polyphasic approach. Cells of strain B6B were rod-shaped, motile and produced ellipsoidal endospores in terminal positions in non-swollen sporangia. Strain B6B was a strictly aerobic bacterium and catalase- and oxidase-positive. The strain was able to grow at NaCl concentrations of 0.5–20.0 % (w/v), with optimum growth occurring at 10.0 % (w/v) NaCl. The optimum temperature and pH for growth were 35 °C and pH 7.0. On the basis of 16S rRNA gene sequence analysis, strain B6B was shown to belong to the phylum and its closest phylogenetic similarities were with the species BH30097 (97.5 %), YIM 93624 (97.4 %), EN8d (96.8 %), NHBX5 (96.6 %), LMG 20964 (96.3 %) and YIM-C158 (96.0 %), respectively. Phylogenetic analysis revealed that strain B6B, along with BH30097 and YIM 93624, clustered in a separate clade in the family . The DNA G+C content of the novel isolate was 35.8 mol%. DNA–DNA hybridization experiments revealed low levels of relatedness between strain B6Band BH30097 (13 %) and YIM 93624 (33 %). The major cellular fatty acid of strain B6B was anteiso-C (75.1 %) and its polar lipid pattern consisted of phosphatidylglycerol, diphosphatidylglycerol, an unknown phospholipid and an unknown glycolipid. The isoprenoid quinones were MK-7 (90 %) and MK-6 (3 %). The peptidoglycan contained -diaminopimelic acid as the diagnostic diamino acid. All of these features support the placement of isolate B6B within the phylum . It is closely related to and , but with features that clearly distinguish it from species of the genus or of other related genera. On the basis of the polyphasic evidence derived in this study, we propose that strain B6B be placed within a new genus, as gen. nov., sp. nov., with B6B as the type strain ( = IBRC-M 10775 = KCTC 13828). We also propose that and should be transferred to this new genus and be named comb. nov. and comb. nov., respectively. The type strain of comb. nov. is BH30097 ( = KCTC 3823 = IBRC-M 10657 = JCM 12387) and the type strain of comb. nov. is YIM 93624 ( = DSM 23711 = IBRC-M 10798 = JCM 17364).

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Funding
This study was supported by the:
  • Research Council, University of Tehran and Iranian Biological Resource Centre (IBRC) (Award MI-1388-04)
  • International Foundation for Science (IFS) (Award A/4527-1)
  • Spanish Ministerio de Economía y Competitividad (Award CGL2013-46941-P)
  • Junta de Andalucía (Award P10-CVI-6226)
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2014-11-01
2024-04-20
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Aquibacillus halophilus gen. nov., sp. nov., a moderately halophilic bacterium from a hypersaline lake, and reclassification of Virgibacillus koreensis as Aquibacillus koreensis comb. nov. and Virgibacillus albus as Aquibacillus albus comb. nov.
Int J Syst Evol Microbiol 64, 3616 (2014); https://doi.org/10.1099/ijs.0.065375-0
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