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Volume 64, Issue Pt_11

sp. nov., a curdlan-degrading bacterium isolated from the junction between the ocean and a freshwater spring, and emended description of the genus Free

Abstract

A Gram-stain-negative, aerobic, non-spore-forming, non-flagellated and rod-shaped bacterial strain, designated DMCK3-4, was isolated from the zone where the ocean and a freshwater spring meet at Jeju island, South Korea. Strain DMCK3-4 grew optimally at 30 °C, at pH 7.0–8.0 and in the presence of 2.0 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences revealed that strain DMCK3-4 clustered with the strains of three members of the genus , with which it exhibited 97.0–99.0 % sequence similarity. Sequence similarities to the type strains of the other species with validly published names were less than 92.2 %. Strain DMCK3-4 contained Q-8 as the predominant ubiquinone and summed feature 3 (Cω7 and/or Cω6), Cω8, C, C and Cω7 as the major fatty acids. The major polar lipids of strain DMCK3-4 were phosphatidylethanolamine, phosphatidylglycerol, two unidentified glycolipids, one unidentified lipid and one unidentified aminolipid. The DNA G+C content of strain DMCK3-4 was 51.8 mol% and its mean DNA–DNA relatedness values with KCTC 23176, KCTC 23293 and NRIC 0917 were 23–34 %, respectively. The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain DMCK3-4 is distinct from other species of the genus . On the basis of the data presented, strain DMCK3-4 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is DMCK3-4 ( = KCTC 42075 = CECT 8570). An emended description of the genus is also proposed.

  • Published Online:
Funding
This study was supported by the:
  • National Institute of Biological Resources (NIBR)
  • Ministry of Environment (MOE)
  • Ministry of Science, ICT & Future Planning (MSIP) of the Republic of Korea (Award grant NRF-2013M3A9A5075953)
© 2014 IUMS
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2014-11-01
2024-04-16
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Simiduia curdlanivorans sp. nov., a curdlan-degrading bacterium isolated from the junction between the ocean and a freshwater spring, and emended description of the genus Simiduia
Int J Syst Evol Microbiol 64, 3695 (2014); https://doi.org/10.1099/ijs.0.065334-0
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