1887

Abstract

A pure culture of an actinobacterium previously described as ‘ Rhodoluna lacicola ’ strain MWH-Ta8 was established and deposited in two public culture collections. Strain MWH-Ta8 represents a free-living planktonic freshwater bacterium obtained from hypertrophic Meiliang Bay, Lake Taihu, PR China. The strain was characterized by phylogenetic and taxonomic investigations, as well as by determination of its complete genome sequence. Strain MWH-Ta8 is noticeable due to its unusually low values of cell size (0.05 µm), genome size (1.43 Mbp), and DNA G+C content (51.5 mol%). Phylogenetic analyses based on 16S rRNA gene and RpoB sequences suggested that strain MWH-Ta8 is affiliated with the family with being its closest relative among the currently described species within this family. Strain MWH-Ta8 and the type strain of shared a 16S rRNA gene sequence similarity of 94.3 %. The cell-wall peptidoglycan of strain MWH-Ta8 was of type B2β (B10), containing 2,4-diaminobutyric acid as the diamino acid. The predominant cellular fatty acids were anteiso-C (36.5 %), iso-C (16.5 %), iso-C (15.6 %) and iso-C (8.9 %), and the major (>10 %) menaquinones were MK-11 and MK-12. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and two unknown glycolipids. The combined phylogenetic, phenotypic and chemotaxonomic data clearly suggest that strain MWH-Ta8 represents a novel species of a new genus in the family , for which the name gen. nov., sp. nov. is proposed. The type strain of the type species is MWH-Ta8 ( = DSM 23834 = LMG 26932).

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2014-09-01
2019-11-13
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