1887

Abstract

A psychrotolerant strain, 8H1, was isolated from soil samples collected in Isla de los Estados, Ushuaia, Argentina. Cells were Gram-negative, aerobic, straight rods, occurring singly or in pairs, non-spore-forming and motile by means of two polar flagella. The isolate was able to grow in the range 4–35 °C, with optimum growth at 28 °C. The predominant cellular fatty acids were summed feature 3 (Cω6 and/or Cω7), C and summed feature 8 (Cω6 and/or Cω7). The polar lipid pattern of strain 8H1 comprised phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and an unknown phospholipid. Ubiquinone 9 (Q-9) was the predominant lipoquinone. The DNA G+C content was 59.8 mol%. 16S rRNA gene sequence-based phylogeny suggested the affiliation of strain 8H1 to the ‘ group’, displaying ≥98.5 % sequence similarity to 29 type strains. A multilocus sequence analysis (MLSA) study performed by concatenating 16S rRNA, , and gene sequences showed that isolate 8H1 could be discriminated from closely related species of the genus and placed in the ‘ subgroup’, including the species with the highest MLSA sequence similarities: (96.2 %), (96.1 %), (96.0 %), (96.0 %) and (95.4 %). DNA–DNA hybridization analysis between 8H1 and the type strains of these closely related species revealed relatedness values of 27.0, 8.8, 41.2, 39.7 and 46.1 %, respectively. These results, together with differences in several phenotypic features, support the classification of a novel species, for which the name sp. nov. is proposed. The type strain is 8H1 ( = DSM 26522 = CCUG 63249 = LMG 27247).

Funding
This study was supported by the:
  • CONICET, Argentina (Award PIP 0460)
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2015-02-01
2021-10-22
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