sp. nov., isolated from a freshwater shrimp culture pond Free

Abstract

A bacterial strain designated CMJ-15 was isolated from a freshwater shrimp culture pond in Taiwan and characterized using a polyphasic taxonomic approach. Cells of strain CMJ-15 were facultatively anaerobic, Gram-stain-negative and motile by a single polar flagellum and formed light-yellow colonies. Growth occurred at 20–40 °C (optimum, 25–30 °C), with 0–0.7 % NaCl (optimum, 0–0.2 %) and at pH 5.0–9.0 (optimum, pH 6.0–7.0). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain CMJ-15 belonged to the genus and its closest neighbour was EM 1, with sequence similarity of 96.3 %. The predominant cellular fatty acids were summed feature 3 (Cω7 and/or Cω6), C and Cω7. The major cellular hydroxy fatty acid was C 3-OH. The polar lipid profile consisted of phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The polyamine profile was composed of the major compound putrescine and moderate amounts of 2-hydroxyputrescine. The major respiratory quinone was Q-8 and the DNA G+C content was 52.2 mol%. On the basis of these phylogenetic and phenotypic data, strain CMJ-15 should be classified as representing a novel species, for which the name sp. nov. is proposed. The type strain is CMJ-15 ( = BCRC 80404 = LMG 26892 = KCTC 23917).

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2014-10-01
2024-03-28
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