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Abstract

The taxonomic status of a rhizospheric soil actinomycete, designated R8-39, was established using a polyphasic approach. The organism had phenotypic and morphological characteristics consistent with its classification in the genus . Phylogenetic analysis based on an almost complete 16S rRNA gene sequence showed that the strain formed a monophyletic clade with the type strains of members of the genus . Strain R8-39 displayed the highest levels of 16S rRNA gene sequence similarity to DSM 44149 (98.8 %) and YIM 120521 (98.3 %). However, the DNA–DNA hybridization values between strain R8-39 and and were clearly below the 70 % threshold. The organism was found to have chemical characteristics consistent with its classification in the genus . Whole-cell hydrolysates contained -diaminopimelic acid, arabinose, galactose, glucose, mannose, rhamnose and ribose. The main menaquinone was MK-9(H). No mycolic acid was detected. The G+C content of the genomic DNA was 71.8 mol%. In addition, strain R8-39 had a phenotypic profile that readily distinguished it from recognized representatives of the genus . It is evident from the combined genotypic and phenotypic properties that strain R8-39 represents a novel species of the genus . The proposed name for this species is sp. nov.; the type strain is R8-39 ( = BCC 60399 = NBRC 109649).

Funding
This study was supported by the:
  • , Kasetsart University Research and Development Institute (KURDI)
  • , Thailand, Office of the Higher Education Commission
  • , Kitasato Institute for Life Sciences, Kitasato University
  • , Faculty of Science, Kasetsart University
  • , Kasetsart University
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2014-10-01
2020-09-26
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