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Abstract

A taxonomic study was carried out on bacterial strain H3, which was isolated from the toxic marine diatom . Cells of strain H3 were Gram-stain-negative, rod-shaped, non-motile and capable of reducing nitrate to nitrite, but not denitrification. Growth was observed at NaCl concentrations of 1–9 %, pH 6–12 and 10–37 °C. It was unable to degrade aesculin or gelatin. The dominant fatty acids (>10 %) were Cω7/ω6 (summed feature 8) and C. The respiratory ubiquinone was Q10. The major lipids were phosphatidylethanolamine, phosphatidylglycerol, an aminolipid and one unknown lipid, and the minor lipids were two phospholipids and three unknown lipids. The G+C content of the chromosomal DNA was 61.7 mol%. 16S rRNA gene sequence comparison showed that strain H3 was related most closely to DSW-25 (97.3 % similarity) and levels of similarity with other species of the genus were 95.1–96.9 %. The mean (±) DNA–DNA hybridization value between strain H3 and DSW-25 was 18.0±2.25 %. The average nucleotide identity between strain H3 and DSW-25 was 70.45 %. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain H3 formed a separate clade close to the genus and was distinguishable from phylogenetically related species by differences in several phenotypic properties. On the basis of the phenotypic and phylogenetic data, strain H3 represents a novel species of the genus , for which the name is proposed (type strain H3 = DSM 26824 = MCCC 1A00686).

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2015-01-01
2019-11-17
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