1887

Abstract

A Gram-negative, coccoid or oval-shaped and gliding bacterial strain, designated HDM-25, belonging to the , was isolated from a tidal flat sediment of the Yellow Sea, Korea, and was subjected to a polyphasic taxonomic study. Strain HDM-25 grew optimally at pH 7.0–8.0, at 30 °C and in the presence of 2–3 % (w/v) NaCl. Neighbour-joining, maximum-likelihood and maximum-parsimony phylogenetic trees based on 16S rRNA gene sequences showed that strain HDM-25 fell within the clade comprising the species of the genus , clustering with the type strain of , with which it exhibited the highest 16S rRNA gene sequence similarity (97.7 %). The 16S rRNA gene sequence similarity between strain HDM-25 and the type strains of the other species of was 93.6–97.0 %. The DNA G+C content was 65.9 mol% and the mean DNA–DNA relatedness between strain HDM-25 and the type strain of was 10.7±2.7 % (9.9±4.0 %, reciprocal analysis). Strain HDM-25 contained Q-10 as the predominant ubiquinone and summed feature 8 (Cω7 and/or Cω6) and C as the major fatty acids. The major polar lipids were phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, an unidentified aminolipid, an unidentified glycolipid and an unidentified lipid. Differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, demonstrated that strain HDM-25 is distinguishable from other species of the genus . On the basis of the data presented, strain HDM-25 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is HDM-25 ( = KCTC 42007 = CECT 8525).

Funding
This study was supported by the:
  • National Institute of Biological Resources (NIBR)
  • Ministry of Environment (MOE), Republic of Korea
  • Program for Collection, Management and Utilization of Biological Resources
  • BK 21 program
  • Ministry of Science, ICT & Future Planning (MSIP) of the Republic of Korea
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2014-08-01
2024-10-04
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References

  1. Barrow G. I., Feltham R. K. A. ( 1993 ). Cowan and Steel’s Manual for the Identification of Medical Bacteria, , 3rd edn.. Cambridge:: Cambridge University Press;. [View Article]
    [Google Scholar]
  2. Baumann P., Baumann L. ( 1981 ). The marine Gram-negative eubacteria: genera Photobacterium, Beneckea, Alteromonas, Pseudomonas, and Alcaligenes . . In The Prokaryotes, pp. 13021331. Edited by Starr M. P., Stolp H., Trüper H. G., Balows A., Schlegel H. G. . Berlin:: Springer;.
    [Google Scholar]
  3. Bowman J. P. ( 2000 ). Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov.. Int J Syst Evol Microbiol 50, 18611868.[PubMed]
    [Google Scholar]
  4. Bruns A., Rohde M., Berthe-Corti L. ( 2001 ). Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. . Int J Syst Evol Microbiol 51, 19972006. [View Article] [PubMed]
    [Google Scholar]
  5. Chen M.-H., Sheu S.-Y., Chen C. A., Wang J.-T., Chen W.-M. ( 2011 ). Paracoccus isoporae sp. nov., isolated from the reef-building coral Isopora palifera . . Int J Syst Evol Microbiol 61, 11381143. [View Article] [PubMed]
    [Google Scholar]
  6. Cohen-Bazire G., Sistrom W. R., Stanier R. Y. ( 1957 ). Kinetic studies of pigment synthesis by nonsulfur purple bacteria. . J Cell Comp Physiol 49, 2568. [View Article]
    [Google Scholar]
  7. Davis D. H., Doudoroff M., Stanier R. Y., Mandel M. ( 1969 ). Proposal to reject the genus Hydrogenomonas: taxonomic implications. . Int J Syst Bacteriol 19, 375390. [View Article]
    [Google Scholar]
  8. Ezaki T., Hashimoto Y., Yabuuchi E. ( 1989 ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39, 224229. [View Article]
    [Google Scholar]
  9. Kim Y.-O., Kong H. J., Park S., Kang S.-J., Kim K.-K., Moon D. Y., Oh T.-K., Yoon J.-H. ( 2010 ). Paracoccus fistulariae sp. nov., a lipolytic bacterium isolated from bluespotted cornetfish, Fistularia commersonii . . Int J Syst Evol Microbiol 60, 29082912. [View Article] [PubMed]
    [Google Scholar]
  10. Kim O.-S., Cho Y.-J., Lee K., Yoon S.-H., Kim M., Na H., Park S.-C., Jeon Y. S., Lee J.-H. & other authors ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716721. [View Article] [PubMed]
    [Google Scholar]
  11. Komagata K., Suzuki K. ( 1987 ). Lipids and cell-wall analysis in bacterial systematics. . Methods Microbiol 19, 161207. [View Article]
    [Google Scholar]
  12. Lányi B. ( 1987 ). Classical and rapid identification methods for medically important bacteria. . Methods Microbiol 19, 167. [View Article]
    [Google Scholar]
  13. Leifson E. ( 1963 ). Determination of carbohydrate metabolism of marine bacteria. . J Bacteriol 85, 11831184.[PubMed]
    [Google Scholar]
  14. Minnikin D. E., Patel P. V., Alshamaony L., Goodfellow M. ( 1977 ). Polar lipid composition in the classification of Nocardia and related bacteria. . Int J Syst Bacteriol 27, 104117. [View Article]
    [Google Scholar]
  15. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. ( 1984 ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2, 233241. [View Article]
    [Google Scholar]
  16. Sasser M. ( 1990 ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE:: MIDI Inc;.
    [Google Scholar]
  17. Sheu S.-Y., Jiang S.-R., Chen C. A., Wang J.-T., Chen W.-M. ( 2011 ). Paracoccus stylophorae sp. nov., isolated from the reef-building coral Stylophora pistillata . . Int J Syst Evol Microbiol 61, 22212226. [View Article] [PubMed]
    [Google Scholar]
  18. Stackebrandt E., Goebel B. M. ( 1994 ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol 44, 846849. [View Article]
    [Google Scholar]
  19. Staley J. T. ( 1968 ). Prosthecomicrobium and Ancalomicrobium: new prosthecate freshwater bacteria. . J Bacteriol 95, 19211942.[PubMed]
    [Google Scholar]
  20. Tamaoka J., Komagata K. ( 1984 ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. . FEMS Microbiol Lett 25, 125128. [View Article]
    [Google Scholar]
  21. Urakami T., Araki H., Oyanagi H., Suzuki K., Komagata K. ( 1990 ). Paracoccus aminophilus sp. nov. and Paracoccus aminovorans sp. nov., which utilize N,N-dimethylformamide. . Int J Syst Bacteriol 40, 287291. [View Article] [PubMed]
    [Google Scholar]
  22. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. & other authors ( 1987 ). Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37, 463464. [View Article]
    [Google Scholar]
  23. Yoon J.-H., Kim H., Kim S.-B., Kim H.-J., Kim W. Y., Lee S. T., Goodfellow M., Park Y.-H. ( 1996 ). Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. . Int J Syst Bacteriol 46, 502505. [View Article]
    [Google Scholar]
  24. Yoon J.-H., Lee S. T., Park Y.-H. ( 1998 ). Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rDNA sequences. . Int J Syst Bacteriol 48, 187194. [View Article] [PubMed]
    [Google Scholar]
  25. Yoon J.-H., Kang K. H., Park Y.-H. ( 2003 ). Psychrobacter jeotgali sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. . Int J Syst Evol Microbiol 53, 449454. [View Article] [PubMed]
    [Google Scholar]
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