RT Journal Article SR Electronic(1) A1 Shahraki, Abdolrazagh Hashemi A1 Çavuşoğlu, Cengiz A1 Borroni, Emanuele A1 Heidarieh, Parvin A1 Koksalan, Orhan Kaya A1 Cabibbe, Andrea Maurizio A1 Hashemzadeh, Mohamad A1 Mariottini, Alessandro A1 Mostafavi, Ehsan A1 Cittaro, Davide A1 Feizabadi, Mohamad Mehdi A1 Lazarevic, Dejan A1 Yaghmaei, Farhad A1 Molinari, Gian Lorenzo A1 Camaggi, Anna A1 Tortoli, EnricoYR 2015 T1 Mycobacterium celeriflavum sp. nov., a rapidly growing scotochromogenic bacterium isolated from clinical specimens JF International Journal of Systematic and Evolutionary Microbiology, VO 65 IS Pt_2 SP 510 OP 515 DO https://doi.org/10.1099/ijs.0.064832-0 PB Microbiology Society, SN 1466-5034, AB Six strains of a rapidly growing scotochromogenic mycobacterium were isolated from pulmonary specimens of independent patients. Biochemical and cultural tests were not suitable for their identification. The mycolic acid pattern analysed by HPLC was different from that of any other mycobacterium. Genotypic characterization, targeting seven housekeeping genes, revealed the presence of microheterogeneity in all of them. Different species were more closely related to the test strains in various regions: the type strain of Mycobacterium moriokaense showed 99.0 % 16S rRNA gene sequence similarity, and 91.5–96.5 % similarity for the remaining six regions. The whole genome sequences of the proposed type strain and that of M. moriokaense presented an average nucleotide identity (ANI) of 82.9 %. Phylogenetic analysis produced poorly robust trees in most genes with the exception of rpoB and sodA where Mycobacterium flavescens and Mycobacterium novocastrense were the closest species. This phylogenetic relatedness was confirmed by the tree inferred from five concatenated genes, which was very robust. The polyphasic characterization of the test strains, supported by the ANI value, demonstrates that they belong to a previously unreported species, for which the name Mycobacterium celeriflavum sp. nov. is proposed. The type strain is AFPC-000207T ( = DSM 46765T = JCM 18439T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.064832-0