1887

Abstract

A halophilic actinomycete, strain R4S8, was isolated from soil of Inche-Broun hypersaline wetland in the north of Iran. The isolate grew aerobically at temperatures of 30–50 °C (optimum 40 °C), pH 6–10 (optimum pH 7.0) and in the presence of 1–15 % (w/v) NaCl (optimum 3–5 %). It formed short and straight to moderately flexuous aerial mycelium without motile elements. The cell wall of strain R4S8 contained -diaminopimelic acid as the diamino acid without any diagnostic sugars. The polar lipid pattern consisted of phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylserine, phosphatidylmonomethylethanolamine two unknown phospholipids and one unknown aminophospholipid. It synthesized anteiso-C (44.8 %), iso-C (28.8 %) and iso-C (8.5 %) as major fatty acids. MK-6 was the predominant respiratory quinone. The G+C content of the genomic DNA was 52.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain R4S8 belongs to the family and showed the closest 16S rRNA gene sequence similarity with IMMIB L-1269 (95.5 %) and SCSIO 10219 (95.3 %). On the basis of phylogenetic analysis and phenotypic characteristics, strain R4S8 represents a novel species in a new genus within the family , for which the name gen. nov., sp. nov. is proposed. The type strain of the type species is R4S8 ( = IBRC-M 10813 = CECT 8506).

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2014-12-01
2019-11-18
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