A novel, moderately thermophilic, bacterial strain (skMP5T) was isolated from sediment of a freshwater lake in Japan. The cells were rod-shaped, motile and Gram-stain-negative. Growth was observed at temperatures ranging from 25 to 52 °C, with optimum growth observed at 48–50 °C. The pH range for growth was pH 5.0–8.2, with optimum growth at pH 6.0–7.0. The G+C content of genomic DNA was 72 mol%. The major components in the fatty acid profile were iso-C17 : 0 and iso-C17 : 1ω9c. The predominant isoprenoid quinone of the strain was ubiquinone Q-8. The strain was facultatively anaerobic, and reduced nitrate to nitrite under anoxic conditions. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolate was a member of the family Xanthomonadaceaewithin the class Gammaproteobacteria, showing highest sequence similarity with Tahibacter aquaticus RaM5-2 (93.6 %) and Metallibacterium scheffleri DKE6T (93.3 %). On the basis of phylogenetic and phenotypic properties, strain skMP5T represents a novel species of a new genus, Mizugakiibacter sediminis gen. nov., sp. nov. The type strain of the type species is skMP5T ( = DSM 27098T = NBRC 109608T).
Katayama-FujimuraY.,
KomatsuY.,
KuraishiH.,
KanekoT.(1984). Estimation of DNA base composition by high-performance liquid chromatography of its nuclease P1 hydrolysate. . Agric Biol Chem48, 3169–3172. [View Article]
KojimaH.,
FukuiM.(2010).Sulfuricella denitrificans gen. nov., sp. nov., a sulfur-oxidizing autotroph isolated from a freshwater lake. . Int J Syst Evol Microbiol60, 2862–2866. [View Article][PubMed]
KojimaH.,
FukuiM.(2011).Sulfuritalea hydrogenivorans gen. nov., sp. nov., a facultative autotroph isolated from a freshwater lake. . Int J Syst Evol Microbiol61, 1651–1655. [View Article][PubMed]
MergaertJ.,
CnockaertM. C.,
SwingsJ.(2002).Fulvimonas soli gen. nov., sp. nov., a gamma-proteobacterium isolated from soil after enrichment on acetylated starch plastic. . Int J Syst Evol Microbiol52, 1285–1289. [View Article][PubMed]
NishijimaM.,
Araki-SakaiM.,
SanoH.(1997). Identification of isoprenoid quinones by frit-FAB liquid chromatography–mass spectrometry for the chemotaxonomy of microorganisms. . J Microbiol Methods28, 113–122. [View Article]
SahaP.,
KrishnamurthiS.,
MayilrajS.,
PrasadG. S.,
BoraT. C.,
ChakrabartiT.(2005).Aquimonas voraii gen. nov., sp. nov., a novel gammaproteobacterium isolated from a warm spring of Assam, India. . Int J Syst Evol Microbiol55, 1491–1495. [View Article][PubMed]
WatanabeM.,
KojimaH.,
FukuiM.(2014). Proposal of Effusibacillus lacus gen. nov., sp. nov., and reclassification of Alicyclobacillus pohliae as Effusibacillus pohliae comb. nov. and Alicyclobacillus consociatus as Effusibacillus consociatus comb. nov.. Int J Syst Evol Microbiol64, 2770–2774. [View Article][PubMed]