1887

Abstract

A novel, moderately thermophilic, bacterial strain (skMP5) was isolated from sediment of a freshwater lake in Japan. The cells were rod-shaped, motile and Gram-stain-negative. Growth was observed at temperatures ranging from 25 to 52 °C, with optimum growth observed at 48–50 °C. The pH range for growth was pH 5.0–8.2, with optimum growth at pH 6.0–7.0. The G+C content of genomic DNA was 72 mol%. The major components in the fatty acid profile were iso-C and iso-Cω9. The predominant isoprenoid quinone of the strain was ubiquinone Q-8. The strain was facultatively anaerobic, and reduced nitrate to nitrite under anoxic conditions. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolate was a member of the family within the class , showing highest sequence similarity with RaM5-2 (93.6 %) and DKE6 (93.3 %). On the basis of phylogenetic and phenotypic properties, strain skMP5 represents a novel species of a new genus, gen. nov., sp. nov. The type strain of the type species is skMP5 ( = DSM 27098 = NBRC 109608).

Funding
This study was supported by the:
  • , Institute for fermentation, Osaka
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.064659-0
2014-12-01
2020-09-25
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/64/12/3983.html?itemId=/content/journal/ijsem/10.1099/ijs.0.064659-0&mimeType=html&fmt=ahah

References

  1. Katayama-Fujimura Y., Komatsu Y., Kuraishi H., Kaneko T. ( 1984 ). Estimation of DNA base composition by high-performance liquid chromatography of its nuclease P1 hydrolysate. . Agric Biol Chem 48, 31693172. [CrossRef]
    [Google Scholar]
  2. Kojima H., Fukui M. ( 2010 ). Sulfuricella denitrificans gen. nov., sp. nov., a sulfur-oxidizing autotroph isolated from a freshwater lake. . Int J Syst Evol Microbiol 60, 28622866. [CrossRef] [PubMed]
    [Google Scholar]
  3. Kojima H., Fukui M. ( 2011 ). Sulfuritalea hydrogenivorans gen. nov., sp. nov., a facultative autotroph isolated from a freshwater lake. . Int J Syst Evol Microbiol 61, 16511655. [CrossRef] [PubMed]
    [Google Scholar]
  4. Kojima H., Iwata T., Fukui M. ( 2009 ). DNA-based analysis of planktonic methanotrophs in a stratified lake. . Freshw Biol 54, 15011509. [CrossRef]
    [Google Scholar]
  5. Kojima H., Watanabe T., Iwata T., Fukui M. ( 2014 ). Identification of major planktonic sulfur oxidizers in stratified freshwater lake. . PLoS ONE 9, e93877. [CrossRef] [PubMed]
    [Google Scholar]
  6. Lane D. J. ( 1991 ). 16S/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 115175. Edited by Stackebrandt E., Goodfellow M. . Chichester:: Wiley;.
    [Google Scholar]
  7. Lovley D. R. ( 2013 ). Dissimilatory Fe(III)- and Mn(IV)-reducing prokaryotes. . In The Prokaryotes, , 4th edn., pp. 287308. Edited by Rosenberg E., DeLong E. F., Lory S., Stackebrandt E., Thompson F. . Berlin:: Springer;. [CrossRef]
    [Google Scholar]
  8. Mergaert J., Cnockaert M. C., Swings J. ( 2002 ). Fulvimonas soli gen. nov., sp. nov., a gamma-proteobacterium isolated from soil after enrichment on acetylated starch plastic. . Int J Syst Evol Microbiol 52, 12851289. [CrossRef] [PubMed]
    [Google Scholar]
  9. Nishijima M., Araki-Sakai M., Sano H. ( 1997 ). Identification of isoprenoid quinones by frit-FAB liquid chromatography–mass spectrometry for the chemotaxonomy of microorganisms. . J Microbiol Methods 28, 113122. [CrossRef]
    [Google Scholar]
  10. Saha P., Krishnamurthi S., Mayilraj S., Prasad G. S., Bora T. C., Chakrabarti T. ( 2005 ). Aquimonas voraii gen. nov., sp. nov., a novel gammaproteobacterium isolated from a warm spring of Assam, India. . Int J Syst Evol Microbiol 55, 14911495. [CrossRef] [PubMed]
    [Google Scholar]
  11. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 27312739. [CrossRef] [PubMed]
    [Google Scholar]
  12. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. ( 1997 ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25, 48764882. [CrossRef] [PubMed]
    [Google Scholar]
  13. Tsutsumi M., Iwata T., Kojima H., Fukui M. ( 2011 ). Spatiotemporal variations in an assemblage of closely related planktonic aerobic methanotrophs. . Freshw Biol 56, 342351. [CrossRef]
    [Google Scholar]
  14. Watanabe M., Kojima H., Fukui M. ( 2013 ). Desulfotomaculum intricatum sp. nov., a sulfate reducer isolated from freshwater lake sediment. . Int J Syst Evol Microbiol 63, 35743578. [CrossRef] [PubMed]
    [Google Scholar]
  15. Watanabe M., Kojima H., Fukui M. ( 2014 ). Proposal of Effusibacillus lacus gen. nov., sp. nov., and reclassification of Alicyclobacillus pohliae as Effusibacillus pohliae comb. nov. and Alicyclobacillus consociatus as Effusibacillus consociatus comb. nov.. Int J Syst Evol Microbiol 64, 27702774. [CrossRef] [PubMed]
    [Google Scholar]
  16. Widdel F., Bak F. ( 1992 ). Gram-negative mesotrophic sulfate-reducing bacteria. . In The Prokaryotes, , 2nd edn., vol. 4, pp. 33523378. Edited by Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K.-H. . New York:: Springer-Verlag;. [CrossRef]
    [Google Scholar]
  17. Xie C.-H., Yokota A. ( 2005 ). Dyella japonica gen. nov., sp. nov., a γ-proteobacterium isolated from soil. . Int J Syst Evol Microbiol 55, 753756. [CrossRef] [PubMed]
    [Google Scholar]
  18. Ziegler S., Waidner B., Itoh T., Schumann P., Spring S., Gescher J. ( 2013 ). Metallibacterium scheffleri gen. nov., sp. nov., an alkalinizing gammaproteobacterium isolated from an acidic biofilm. . Int J Syst Evol Microbiol 63, 14991504. [CrossRef] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.064659-0
Loading
/content/journal/ijsem/10.1099/ijs.0.064659-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error