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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 64, Issue Pt_12

gen. nov., sp. nov., isolated from a freshwater lake Free

Abstract

A novel, moderately thermophilic, bacterial strain (skMP5) was isolated from sediment of a freshwater lake in Japan. The cells were rod-shaped, motile and Gram-stain-negative. Growth was observed at temperatures ranging from 25 to 52 °C, with optimum growth observed at 48–50 °C. The pH range for growth was pH 5.0–8.2, with optimum growth at pH 6.0–7.0. The G+C content of genomic DNA was 72 mol%. The major components in the fatty acid profile were iso-C and iso-Cω9. The predominant isoprenoid quinone of the strain was ubiquinone Q-8. The strain was facultatively anaerobic, and reduced nitrate to nitrite under anoxic conditions. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolate was a member of the family within the class , showing highest sequence similarity with RaM5-2 (93.6 %) and DKE6 (93.3 %). On the basis of phylogenetic and phenotypic properties, strain skMP5 represents a novel species of a new genus, gen. nov., sp. nov. The type strain of the type species is skMP5 ( = DSM 27098 = NBRC 109608).

  • Published Online:
Funding
This study was supported by the:
  • Institute for fermentation, Osaka
© 2014 IUMS
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2014-12-01
2025-04-19
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Mizugakiibacter sediminis gen. nov., sp. nov., isolated from a freshwater lake
Int J Syst Evol Microbiol 64, 3983 (2014); https://doi.org/10.1099/ijs.0.064659-0
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