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Abstract

A novel strain, designated 5GHs31-2, was isolated from a greenhouse soil sample collected from Yongin city, South Korea. Cells of strain 5GHs31-2 were Gram-stain-negative, rod-shaped, polar flagellated and yellow-pigmented. The isolate was aerobic, catalase-negative and oxidase-positive and grew optimally at 28–30 °C and pH 7.0. Strain 5GHs31-2 revealed the highest 16S rRNA gene sequence similarities with LMG 19981 (97.6 %), ATSB10 (97.5 %) and VA24 (97.4 %). Furthermore, the neighbour-joining tree showed that strain 5GHs31-2 was a representative of a member of the genus . Strain 5GHs31-2 contained iso-C, summed feature 9 (iso-Cω9 and/or C 10-methyl), iso-C and iso-C as the major fatty acids, phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and an aminolipid as the main polar lipids and Q-8 as the predominant quinone. The genomic DNA G+C content of 5GHs31-2 was 73.0 mol%. According to DNA–DNA hybridization experiments, DNA–DNA relatedness values between strain 5GHs31-2 and its closest phylogenetic neighbours were below 70 %. Based on the taxonomic data, strain 5GHs31-2 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 5GHs31-2 ( = KACC 16952 = DSM 28344).

Funding
This study was supported by the:
  • , National Academy of Agricultural Science, Rural Development Administration, Republic of Korea , (Award PJ008666)
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2014-08-01
2020-11-26
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