A Gram-staining-negative, rod-shaped, motile and facultatively anaerobic bacterial strain, designated X2T, was isolated from the sludge of an anaerobic, denitrifying, sulfide-removal bioreactor, and found to oxidize sulfide anaerobically with nitrate as electron acceptor. The strain grew at salinities of 0–3 % (w/v) NaCl (optimum, 0–1 %). Growth occurred at pH 6.0–10.0 (optimum, pH 8.0) and 10–37 °C (optimum, 30 °C). The genomic DNA G+C content was 59 mol%. Q-8 and Q-9 were detected as the respiratory quinones. The major fatty acids (>10 %) were C16 : 1ω7c and/or C16 : 1ω6c, C18 : 1ω7c and C16 : 0. The polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and one unidentified phospholipid. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain X2T formed a novel clade within the family Pseudomonadaceae, with the highest sequence similarity to Pseudomonas caeni KCTC 22292T (93.5 %). On the basis of phenotypic, chemotaxonomic and phylogenetic characteristics, it is proposed that this strain represents novel genus and species within the family Pseudomonadaceae, for which the name Thiopseudomonas denitrificans gen. nov., sp. nov. is proposed. The type strain is X2T ( = CCTCC M 2013362T = DSM 28679T = KCTC 42076T).
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Funding
This study was supported by the:
National High-tech R & D Program of China
(Award 2011AA060904)
National Science Foundation for Distinguished Young Scholars
(Award 51225802)
National Natural Science Foundation of China
(Award 51121062)
Ministry of Science and Technology of China
(Award 2010BAC67B02)
National Natural Science Foundation of China
(Award 51308147)
Guangdong Province Higher Vocational Colleges & Schools Pearl River Scholar Funded Scheme
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