1887

Abstract

A Gram-staining-negative, rod-shaped, motile and facultatively anaerobic bacterial strain, designated X2, was isolated from the sludge of an anaerobic, denitrifying, sulfide-removal bioreactor, and found to oxidize sulfide anaerobically with nitrate as electron acceptor. The strain grew at salinities of 0–3 % (w/v) NaCl (optimum, 0–1 %). Growth occurred at pH 6.0–10.0 (optimum, pH 8.0) and 10–37 °C (optimum, 30 °C). The genomic DNA G+C content was 59 mol%. Q-8 and Q-9 were detected as the respiratory quinones. The major fatty acids (>10 %) were Cω7 and/or Cω6, Cω7 and C. The polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and one unidentified phospholipid. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain X2 formed a novel clade within the family , with the highest sequence similarity to KCTC 22292 (93.5 %). On the basis of phenotypic, chemotaxonomic and phylogenetic characteristics, it is proposed that this strain represents novel genus and species within the family , for which the name gen. nov., sp. nov. is proposed. The type strain is X2 ( = CCTCC M 2013362 = DSM 28679 = KCTC 42076).

Funding
This study was supported by the:
  • National High-tech R & D Program of China (Award 2011AA060904)
  • National Science Foundation for Distinguished Young Scholars (Award 51225802)
  • National Natural Science Foundation of China (Award 51121062)
  • Ministry of Science and Technology of China (Award 2010BAC67B02)
  • National Natural Science Foundation of China (Award 51308147)
  • Guangdong Province Higher Vocational Colleges & Schools Pearl River Scholar Funded Scheme
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2015-01-01
2021-10-17
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