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Volume 64, Issue Pt_8

Descriptions of gen. nov., sp. nov., a bacteriochlorophyll -containing fermentative bacterium related phylogenetically to alphaproteobacterial methanotrophs, and of the family fam. nov. Free

Abstract

A light-pink-pigmented, microaerophilic bacterium was obtained from a methanotrophic consortium enriched from acidic peat and designated strain Pf56. Cells of this bacterium were Gram-negative, non-motile, thick curved rods that contained a vesicular intracytoplasmic membrane system characteristic of some purple non-sulfur alphaproteobacteria. The absorption spectrum of acetone/methanol extracts of cells grown in the light showed maxima at 363, 475, 505, 601 and 770 nm; the peaks at 363 and 770 nm are characteristic of bacteriochlorophyll . However, in contrast to purple non-sulfur bacteria, strain Pf56 was unable to grow phototrophically under anoxic conditions in the light. Best growth occurred on some sugars and organic acids under micro-oxic conditions by means of fermentation. The fermentation products were propionate, acetate and hydrogen. Slow chemo-organotrophic growth was also observed under fully oxic conditions. Light stimulated growth. C substrates were not utilized. Strain Pf56 grew at pH 4.0–7.0 (optimum pH 5.5–6.5) and at 15–30 °C (optimum 22–28 °C). The major cellular fatty acids were 19 : 0 cyclo ω8 and 18 : 1ω7; quinones were represented by ubiquinone Q-10. The G+C content of the DNA was 70.0 mol%. Strain Pf56 displays 93.6–94.7 and 92.7–93.7 % 16S rRNA gene sequence similarity to members of the families and , respectively, and belongs to a large cluster of environmental sequences retrieved from various wetlands and forest soils in cultivation-independent studies. Phenotypic, genotypic and chemotaxonomic characteristics of strain Pf56 suggest that it represents a novel genus and species of bacteriochlorophyll -containing fermentative bacteria, for which the name gen. nov., sp. nov. is proposed. Strain Pf56 ( = DSM 24875 = VKM B-2876) is the type strain of , and is also the first characterized member of a novel family within the class , fam. nov.

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Funding
This study was supported by the:
  • Program ‘Molecular and Cell Biology’
  • Russian Fund of Basic Research (Award 12-04-00768)
© 2014 IUMS
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2014-08-01
2024-04-24
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Descriptions of Roseiarcus fermentans gen. nov., sp. nov., a bacteriochlorophyll a-containing fermentative bacterium related phylogenetically to alphaproteobacterial methanotrophs, and of the family Roseiarcaceae fam. nov.
Int J Syst Evol Microbiol 64, 2558 (2014); https://doi.org/10.1099/ijs.0.064576-0
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