1887

Abstract

Ten isolates of unknown, Gram-stain-negative, anaerobic cocci were recovered from human clinical samples, mainly from semen. On the basis of their phenotypic features, including morphology, main metabolic end products, gas production, nitrate reduction and decarboxylation of succinate, the strains were identified as members of the genus Multi-locus sequence analysis and corresponding phylogenies were based on 16S rRNA, and genes, and on the newly proposed gene. The strains shared high levels of genetic sequence similarity and were related most closely to . The strains could not be differentiated from on the basis of 16S rRNA gene sequence analysis while , and gene sequences showed 85.1, 93.5 and 90.2 % similarity with those of the type strain of , respectively. Phylogenetic analyses revealed that the isolates formed a robust clade in the subgroup of the genus . As observed for , the isolates were able to ferment fructose. In contrast to other members of the genus , the 10 strains were not able to metabolize lactate. Cellular fatty acid composition was consistent with that of other species of the genus . From these data, the 10 isolates are considered to belong to a novel species in the genus , for which the name sp. nov. is proposed. The type strain is ADV 4313.2 ( = CIP 107810 = LMG 28162). strain ACS-216-V-Col6b subjected to whole genome sequencing as part as the Human Microbiome Project is another representative of sp. nov. An emended description of the genus is also proposed.

Funding
This study was supported by the:
  • Ministère de l’enseignement supérieur et de la recherche
  • ADEREMPHA
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.064451-0
2014-10-01
2024-12-14
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/64/10/3526.html?itemId=/content/journal/ijsem/10.1099/ijs.0.064451-0&mimeType=html&fmt=ahah

References

  1. Arif N., Do T., Byun R., Sheehy E., Clark D., Gilbert S. C., Beighton D. ( 2008 ). Veillonella rogosae sp. nov., an anaerobic, Gram-negative coccus isolated from dental plaque. . Int J Syst Evol Microbiol 58, 581584. [View Article] [PubMed]
    [Google Scholar]
  2. Beighton D., Clark D., Hanakuka B., Gilbert S., Do T. ( 2008 ). The predominant cultivable Veillonella spp. of the tongue of healthy adults identified using rpoB sequencing. . Oral Microbiol Immunol 23, 344347. [View Article] [PubMed]
    [Google Scholar]
  3. Byun R., Carlier J.-P., Jacques N. A., Marchandin H., Hunter N. ( 2007 ). Veillonella denticariosi sp. nov., isolated from human carious dentine. . Int J Syst Evol Microbiol 57, 28442848. [View Article] [PubMed]
    [Google Scholar]
  4. Carlier J.-P. ( 1985 ). Gas chromatography of fermentation products: its application in diagnosis of anaerobic bacteria. . Bull Inst Pasteur 83, 5769.
    [Google Scholar]
  5. Carlier J.-P. ( 2009 ). Genus I. Veillonella . . In Bergey’s Manual of Systematic Bacteriology, , 2nd edn., vol. 3, pp. 10391045. Edited by De Vos P., Garrity G., Jones D., Krieg N. R., Ludwig W., Rainey F. A., Schleifer K.-H., Whitman W. B. . New York:: Springer;.
    [Google Scholar]
  6. Carlier J.-P., Marchandin H., Jumas-Bilak E., Lorin V., Henry C., Carrière C., Jean-Pierre H. ( 2002 ). Anaeroglobus geminatus gen. nov., sp. nov., a novel member of the family Veillonellaceae . . Int J Syst Evol Microbiol 52, 983986. [View Article] [PubMed]
    [Google Scholar]
  7. Dereeper A., Guignon V., Blanc G., Audic S., Buffet S., Chevenet F., Dufayard J.-F., Guindon S., Lefort V. et al. ( 2008 ). Phylogeny.fr: robust phylogenetic analysis for the non-specialist. . Nucleic Acids Res 36 (Web Server issue), W465W469. [View Article] [PubMed]
    [Google Scholar]
  8. Hall T. A. ( 1999 ). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. . Nucleic Acids Symp Ser 41, 9598.
    [Google Scholar]
  9. Holdeman L. V., Cato E. P., Moore W. E. C. ( 1977 ). Anaerobe Laboratory Manual, , 4th edn.. Blacksburg, VA:: Virginia Polytechnic Institute/State University;.
    [Google Scholar]
  10. Jousimies-Somer H. R., Summanen P., Citron D. M., Baron E. J., Wexler H. M., Finegold S. M. ( 2002 ). Wadworth-KTL Anaerobic Bacteriology Manual, , 6th edn.. Belmont, CA:: Star Publishing Company;.
    [Google Scholar]
  11. Jumas-Bilak E., Carlier J.-P., Jean-Pierre H., Teyssier C., Gay B., Campos J., Marchandin H. ( 2004 ). Veillonella montpellierensis sp. nov., a novel, anaerobic, Gram-negative coccus isolated from human clinical samples. . Int J Syst Evol Microbiol 54, 13111316. [View Article] [PubMed]
    [Google Scholar]
  12. Kraatz M., Taras D. ( 2008 ). Veillonella magna sp. nov., isolated from the jejunal mucosa of a healthy pig, and emended description of Veillonella ratti . . Int J Syst Evol Microbiol 58, 27552761. [View Article] [PubMed]
    [Google Scholar]
  13. Marchandin H., Jumas-Bilak E. ( 2014 ). Family Veillonellaceae . . In The Prokaryotes, , 4th edn., vol. 7. Edited by Rosenberg E. , DeLong E. F., Lory S. , Stackebrandt E. , Thompson F. . Berlin:: Springer;.
    [Google Scholar]
  14. Marchandin H., Teyssier C., Siméon De Buochberg M., Jean-Pierre H., Carrière C., Jumas-Bilak E. ( 2003 ). Intra-chromosomal heterogeneity between the four 16S rRNA gene copies in the genus Veillonella: implications for phylogeny and taxonomy. . Microbiology 149, 14931501. [View Article] [PubMed]
    [Google Scholar]
  15. Marchandin H., Teyssier C., Jumas-Bilak E., Robert M., Artigues A.-C., Jean-Pierre H. ( 2005 ). Molecular identification of the first human isolate belonging to the Veillonella rattiVeillonella criceti group based on 16S rDNA and dnaK gene sequencing. . Res Microbiol 156, 603607. [View Article] [PubMed]
    [Google Scholar]
  16. Marchandin H., Teyssier C., Campos J., Jean-Pierre H., Roger F., Gay B., Carlier J.-P., Jumas-Bilak E. ( 2010 ). Negativicoccus succinicivorans gen. nov., sp. nov., isolated from human clinical samples, emended description of the family Veillonellaceae and description of Negativicutes classis nov., Selenomonadales ord. nov. and Acidaminococcaceae fam. nov. in the bacterial phylum Firmicutes . . Int J Syst Evol Microbiol 60, 12711279. [View Article] [PubMed]
    [Google Scholar]
  17. Mashima I., Kamaguchi A., Nakazawa F. ( 2011 ). The distribution and frequency of oral Veillonella spp. in the tongue biofilm of healthy young adults. . Curr Microbiol 63, 403407. [View Article] [PubMed]
    [Google Scholar]
  18. Mashima I., Kamaguchi A., Miyakawa H., Nakazawa F. ( 2013 ). Veillonella tobetsuensis sp. nov., an anaerobic, gram-negative coccus isolated from human tongue biofilms. . Int J Syst Evol Microbiol 63, 14431449. [View Article] [PubMed]
    [Google Scholar]
  19. Mays T. D., Holdeman L. V., Moore W. E. C., Rogosa M., Johnson J. L. ( 1982 ). Taxonomy of the genus Veillonella Prévot. . Int J Syst Bacteriol 32, 2836. [View Article]
    [Google Scholar]
  20. Michon A.-L., Aujoulat F., Roudière L., Soulier O., Zorgniotti I., Jumas-Bilak E., Marchandin H. ( 2010 ). Intragenomic and intraspecific heterogeneity in rrs may surpass interspecific variability in a natural population of Veillonella . . Microbiology 156, 20802091. [View Article] [PubMed]
    [Google Scholar]
  21. Prévot A. R. ( 1933 ). Etude de systématique bactérienne. I. Lois générales. II. Cocci anaérobius. . Annales des Sciences Naturelles Zoologie et Biologie Animale 15, 23260.
    [Google Scholar]
  22. Rogosa M. ( 1984 ). Anaerobic Gram-negative cocci. Family I. Veillonellaceae Rogosa 1971. . In Bergey’s Manual of Systematic Bacteriology, vol. 1, pp. 680685. Edited by Krieg N. R., Holt J. G. . Baltimore:: Williams & Wilkins;.
    [Google Scholar]
/content/journal/ijsem/10.1099/ijs.0.064451-0
Loading
/content/journal/ijsem/10.1099/ijs.0.064451-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error