1887

Abstract

Ten isolates of unknown, Gram-stain-negative, anaerobic cocci were recovered from human clinical samples, mainly from semen. On the basis of their phenotypic features, including morphology, main metabolic end products, gas production, nitrate reduction and decarboxylation of succinate, the strains were identified as members of the genus . Multi-locus sequence analysis and corresponding phylogenies were based on 16S rRNA, and genes, and on the newly proposed gene. The strains shared high levels of genetic sequence similarity and were related most closely to . The strains could not be differentiated from on the basis of 16S rRNA gene sequence analysis while , and gene sequences showed 85.1, 93.5 and 90.2 % similarity with those of the type strain of , respectively. Phylogenetic analyses revealed that the isolates formed a robust clade in the subgroup of the genus . As observed for , the isolates were able to ferment fructose. In contrast to other members of the genus , the 10 strains were not able to metabolize lactate. Cellular fatty acid composition was consistent with that of other species of the genus . From these data, the 10 isolates are considered to belong to a novel species in the genus , for which the name sp. nov. is proposed. The type strain is ADV 4313.2 ( = CIP 107810 = LMG 28162). strain ACS-216-V-Col6b subjected to whole genome sequencing as part as the Human Microbiome Project is another representative of sp. nov. An emended description of the genus is also proposed.

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2014-10-01
2019-11-21
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