1887

Abstract

Two anaerobic bacterial strains, MB9-7 and MB9-9, were isolated from decomposing algal scum and were characterized using a polyphasic approach. Phylogenetic analysis of 16S rRNA gene sequences showed that strains MB9-7 and MB9-9 are closely related to each other (99.7 % similarity) and they are also closely related to (96.5 %). The two strains were Gram-stain positive and rod-shaped. Growth occurred at 20–45 °C, at pH 4.0–8.0 and at NaCl concentrations of up to 2 % (w/v). Acid was produced from glucose, xylose and mannose. Products of fermentation in PYG medium were mainly butyrate, acetate, carbon dioxide and hydrogen. The predominant cellular fatty acids were C and C. The cellular polar lipids comprised phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, two glycolipids, one phospholipid, one aminophospholipid and two aminolipids. The DNA G+C contents of strain MB9-7 and MB9-9 were 27.9 and 28.7 mol%, respectively. These results support the assignment of the new isolates to the genus and also distinguish them from other species of the genus . Hence, it is proposed that strains MB9-7 and MB9-9 represent a novel species of the genus with the suggested name sp. nov. The type strain is MB9-7 ( = CGMCC 1.5188 = DSM 28783).

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2014-11-01
2019-11-20
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