1887

Abstract

Two anaerobic bacterial strains, MB9-7 and MB9-9, were isolated from decomposing algal scum and were characterized using a polyphasic approach. Phylogenetic analysis of 16S rRNA gene sequences showed that strains MB9-7 and MB9-9 are closely related to each other (99.7 % similarity) and they are also closely related to (96.5 %). The two strains were Gram-stain positive and rod-shaped. Growth occurred at 20–45 °C, at pH 4.0–8.0 and at NaCl concentrations of up to 2 % (w/v). Acid was produced from glucose, xylose and mannose. Products of fermentation in PYG medium were mainly butyrate, acetate, carbon dioxide and hydrogen. The predominant cellular fatty acids were C and C. The cellular polar lipids comprised phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, two glycolipids, one phospholipid, one aminophospholipid and two aminolipids. The DNA G+C contents of strain MB9-7 and MB9-9 were 27.9 and 28.7 mol%, respectively. These results support the assignment of the new isolates to the genus and also distinguish them from other species of the genus . Hence, it is proposed that strains MB9-7 and MB9-9 represent a novel species of the genus with the suggested name sp. nov. The type strain is MB9-7 ( = CGMCC 1.5188 = DSM 28783).

Funding
This study was supported by the:
  • , National Science Foundation of China , (Award U1202231)
  • , State Key Laboratory of Microbial Resources at the Institute of Microbiology, Chinese Academy of Sciences , (Award SKLMR-20110605)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.064345-0
2014-11-01
2020-09-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/64/11/3844.html?itemId=/content/journal/ijsem/10.1099/ijs.0.064345-0&mimeType=html&fmt=ahah

References

  1. Collins M. D., Lawson P. A., Willems A., Cordoba J. J., Fernandez-Garayzabal J., Garcia P., Cai J., Hippe H., Farrow J. A. E. ( 1994 ). The phylogeny of the genus Clostridium: proposal of five new genera and eleven new species combinations. . Int J Syst Bacteriol 44, 812826. [CrossRef] [PubMed]
    [Google Scholar]
  2. Felsenstein J. ( 1985 ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39, 783791. [CrossRef]
    [Google Scholar]
  3. Fitch W. M. ( 1971 ). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20, 406416. [CrossRef]
    [Google Scholar]
  4. Holdeman L. V., Cato E. P., Moore W. E. C. (editors) ( 1977 ). Anaerobe Laboratory Manual, , 4th edn. . Blacksburg, VA:: Virginia Polytechnic Institute and State University;.
    [Google Scholar]
  5. Hungate R. E. ( 1969 ). A roll-tube method for the cultivation of strict anaerobes. . Methods Microbiol 3B, 117132. [CrossRef]
    [Google Scholar]
  6. Inglett K. S., Bae H. S., Aldrich H. C., Hatfield K., Ogram A. V. ( 2011 ). Clostridium chromiireducens sp. nov., isolated from Cr(VI)-contaminated soil. . Int J Syst Evol Microbiol 61, 26262631. [CrossRef] [PubMed]
    [Google Scholar]
  7. Johnson M. J., Thatcher E., Cox M. E. ( 1995 ). Techniques for controlling variability in gram staining of obligate anaerobes. . J Clin Microbiol 33, 755758.[PubMed]
    [Google Scholar]
  8. Keis S., Shaheen R., Jones D. T. ( 2001 ). Emended descriptions of Clostridium acetobutylicum and Clostridium beijerinckii, and descriptions of Clostridium saccharoperbutylacetonicum sp. nov. and Clostridium saccharobutylicum sp. nov.. Int J Syst Evol Microbiol 51, 20952103. [CrossRef] [PubMed]
    [Google Scholar]
  9. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. & other authors ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716721. [CrossRef] [PubMed]
    [Google Scholar]
  10. Kimura M. ( 1980 ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16, 111120. [CrossRef]
    [Google Scholar]
  11. Lin P. Y., Whang L. M., Wu Y. R., Ren W. J., Hsiao C. J., Li S. L., Chang J. S. ( 2007 ). Biological hydrogen production of the genus Clostridium: metabolic study and mathematical model simulation. . Int J Hydrogen Energy 32, 17281735. [CrossRef]
    [Google Scholar]
  12. Marmur J., Doty P. ( 1962 ). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. . J Mol Biol 5, 109118. [CrossRef] [PubMed]
    [Google Scholar]
  13. Ruusunen M., Surakka A., Korkeala H., Lindström M. ( 2012 ). Clostridium tyrobutyricum strains show wide variation in growth at different NaCl, pH, and temperature conditions. . J Food Prot 75, 17911795. [CrossRef] [PubMed]
    [Google Scholar]
  14. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  15. Schaeffer A. B., Fulton M. D. ( 1933 ). A simplified method of staining endospores. . Science 77, 194. [CrossRef] [PubMed]
    [Google Scholar]
  16. Shiratori H., Sasaya K., Ohiwa H., Ikeno H., Ayame S., Kataoka N., Miya A., Beppu T., Ueda K. ( 2009 ). Clostridium clariflavum sp. nov. and Clostridium caenicola sp. nov., moderately thermophilic, cellulose-/cellobiose-digesting bacteria isolated from methanogenic sludge. . Int J Syst Evol Microbiol 59, 17641770. [CrossRef] [PubMed]
    [Google Scholar]
  17. Suresh K., Prakash D., Rastogi N., Jain R. K. ( 2007 ). Clostridium nitrophenolicum sp. nov., a novel anaerobic p-nitrophenol-degrading bacterium, isolated from a subsurface soil sample. . Int J Syst Evol Microbiol 57, 18861890. [CrossRef] [PubMed]
    [Google Scholar]
  18. Tamura K., Dudley J., Nei M., Kumar S. ( 2007 ). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. . Mol Biol Evol 24, 15961599. [CrossRef] [PubMed]
    [Google Scholar]
  19. Tanner R. S., Miller L. M., Yang D. ( 1993 ). Clostridium ljungdahlii sp. nov., an acetogenic species in clostridial rRNA homology group I. . Int J Syst Bacteriol 43, 232236. [CrossRef] [PubMed]
    [Google Scholar]
  20. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. ( 1997 ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25, 48764882. [CrossRef] [PubMed]
    [Google Scholar]
  21. Wiegel J., Tanner R., Rainey F. A. ( 2006 ). An introduction to the family Clostridiaceae . . In The Prokaryotes: a Handbook on the Biology of Bacteria, , 3rd edn., vol. 4, pp. 654678. Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K. H., Stackebrandt E. . New York:: Springer;. [CrossRef]
    [Google Scholar]
  22. Wu Y. F., Wu Q. L., Liu S. J. ( 2012 ). Chryseobacterium taihuense sp. nov., isolated from a eutrophic lake and emended descriptions of the genus Chryseobacterium, C. taiwanense, C. jejuense and C. indoltheticum . . Int J Syst Evol Microbiol 63, 913919. [CrossRef] [PubMed]
    [Google Scholar]
  23. Zhang J. Y., Liu X. Y., Liu S. J. ( 2011 ). Phycicoccus cremeus sp. nov., isolated from forest soil, and emended description of the genus Phycicoccus . . Int J Syst Evol Microbiol 61, 7175. [CrossRef] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.064345-0
Loading
/content/journal/ijsem/10.1099/ijs.0.064345-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error