1887

Abstract

A novel Gram-stain-positive, spore-forming, aerobic, non-motile, rod-shaped bacterium designated strain SD111 that forms red-pigmented colonies was isolated from a marine sediment sample (collected from 5 m depth) from Lakshadweep, India. Strain SD111 grew well on seawater agar at pH 6–10 (optimum pH 7.5±0.2). It showed maximum (97.6 %) 16S rRNA gene sequence similarity and formed a monophyletic clade with WS 4628 ( = DSM 25058). The genomic DNA G+C content was 37.4 mol% and the strain showed 37.7 % DNA–DNA relatedness to DSM 25058. The major fatty acids were anteiso-C, C, iso-C and iso-C and MK-6 was the predominant quinone. The polar lipid profile of strain SD111 consisted of unidentified phospholipids (PL1 and PL2), phosphatidylglycerol (PG) and diphosphatidylglycerol (DPG). The cell wall contained -diaminopimelic acid and the peptidoglycan was of A1γ type. Glucose and ribose were detected as major cell-wall sugars. Results from polyphasic studies indicated that SD111 represents a novel species of the genus for which the name sp. nov. is proposed. The type strain is SD111 ( = MCC 2255 = DSM 28032).

Funding
This study was supported by the:
  • , Department of Biotechnology (DBT), Government of India , (Award BT/PR/0054/NDB/52/94/2007)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.064295-0
2014-09-01
2020-09-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/64/9/3010.html?itemId=/content/journal/ijsem/10.1099/ijs.0.064295-0&mimeType=html&fmt=ahah

References

  1. De Ley J., Cattoir H., Reynaerts A. ( 1970 ). The quantitative measurement of DNA hybridization from renaturation rates. . Eur J Biochem 12, 133142. [CrossRef] [PubMed]
    [Google Scholar]
  2. Felsenstein J. ( 1981 ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17, 368376. [CrossRef] [PubMed]
    [Google Scholar]
  3. Felsenstein J. ( 1985 ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39, 783791. [CrossRef]
    [Google Scholar]
  4. Gillis M., De Ley J., De Cleene M. ( 1970 ). The determination of molecular weight of bacterial genome DNA from renaturation rates. . Eur J Biochem 12, 143153. [CrossRef] [PubMed]
    [Google Scholar]
  5. Gonzalez J. M., Saiz-Jimenez C. ( 2002 ). A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. . Environ Microbiol 4, 770773. [CrossRef] [PubMed]
    [Google Scholar]
  6. Huss V. A. R., Festl H., Schleifer K. H. ( 1983 ). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. . Syst Appl Microbiol 4, 184192. [CrossRef] [PubMed]
    [Google Scholar]
  7. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. & other authors ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716721. [CrossRef] [PubMed]
    [Google Scholar]
  8. Kimura M. ( 1980 ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16, 111120. [CrossRef] [PubMed]
    [Google Scholar]
  9. Lane D. J. ( 1991 ). 16S/23S rRNA sequencing. . In Nucleic Acids Techniques in Bacterial Systematics, pp. 115147. Edited by Stackebrandt E., Goodfellow M. . Chichester:: Wiley;.
    [Google Scholar]
  10. Larkin M. A., Blackshields G., Brown N. P., Chenna R., McGettigan P. A., McWilliam H., Valentin F., Wallace I. M., Wilm A. & other authors ( 2007 ). clustal w and clustal_x version 2.0. . Bioinformatics 23, 29472948. [CrossRef] [PubMed]
    [Google Scholar]
  11. Loveland-Curtze J., Miteva V. I., Brenchley J. E. ( 2011 ). Evaluation of a new fluorimetric DNA–DNA hybridization method. . Can J Microbiol 57, 250255. [CrossRef] [PubMed]
    [Google Scholar]
  12. Marmur J. ( 1961 ). A procedure for isolation of deoxyribonucleic acid from micro-organisms. . J Mol Biol 3, 208218. [CrossRef]
    [Google Scholar]
  13. Nei M., Kumar S. ( 2000 ). Molecular Evolution and Phylogenetics. New York:: Oxford University Press;.
    [Google Scholar]
  14. Parte A. C. ( 2014 ). LPSN–list of prokaryotic names with standing in nomenclature. . Nucleic Acids Res 42 (Database issue), D613D616. [CrossRef] [PubMed]
    [Google Scholar]
  15. Prakash O., Green S. J., Jasrotia P., Overholt W. A., Canion A., Watson D. B., Brooks S. C., Kostka J. E. ( 2012 ). Rhodanobacter denitrificans sp. nov., isolated from nitrate-rich zones of a contaminated aquifer. . Int J Syst Evol Microbiol 62, 24572462. [CrossRef] [PubMed]
    [Google Scholar]
  16. Prakash O., Munot H., Nimonkar Y., Sharma M., Kumbhare S., Shouche Y. S. ( 2014 ). Description of Pelistega indica sp. nov., isolated from human gut. . Int J Syst Evol Microbiol 64, 13891394. [CrossRef] [PubMed]
    [Google Scholar]
  17. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  18. Schumann P. ( 2011 ). Peptidoglycan structure. . In Taxonomy of Prokaryotes, Methods in Microbiology, vol. 38, pp. 101129. Edited by Rainey F., Oren A. . London:: Academic Press;. [CrossRef]
    [Google Scholar]
  19. Seiler H., Wenning M., Scherer S. ( 2013 ). Domibacillus robiginosus gen. nov., sp. nov., isolated from a pharmaceutical clean room. . Int J Syst Evol Microbiol 63, 20542061. [CrossRef] [PubMed]
    [Google Scholar]
  20. Smibert R. M., Krieg N. R. ( 1994 ). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607654. Edited by Gerhardt P. . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  21. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 27312739. [CrossRef] [PubMed]
    [Google Scholar]
  22. Tindall B. J. ( 1990a ). Lipid composition of Halobacterium lacusprofundi . . FEMS Microbiol Lett 66, 199202. [CrossRef]
    [Google Scholar]
  23. Tindall B. J. ( 1990b ). A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. . Syst Appl Microbiol 13, 128130. [CrossRef]
    [Google Scholar]
  24. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. & other authors ( 1987 ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37, 463464. [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.064295-0
Loading
/content/journal/ijsem/10.1099/ijs.0.064295-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error