A Gram-reaction-negative, aerobic, rod-shaped (1.2–1.6 µm×0.6–0.8 µm), flagellated and motile marine bacterium, designated MEBiC06243T, was isolated from a sediment collected at Daebu Island in the Yellow Sea (37° 20′ N 126° 41′ E), Korea. The 16S rRNA gene sequence analysis revealed that strain MEBiC06243T showed high similarity with Neptunomonas naphthovorans NAG-2N-126T (96.3 %). Growth was observed at 10–39 °C (optimum 29 °C), at pH 6.0–9.0 (optimum pH 7) and with 0–7 % (optimum 2.5 %) NaCl. The predominant cellular fatty acids were C10 : 0 3-OH (6.1 %), C12 : 0 (5.8 %), C16 : 0 (30.5 %), C18 : 1ω7c (21.6 %) and summed feature 3 (comprising C15 : 0 2-OH and/or C16 : 1ω7c; 30.7 %). The DNA G+C content was 41.4 mol%. The major respiratory quinone was Q-8. Phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, two unidentified lipids, one unidentified aminophospholipid and three unidentified aminolipids were detected as major polar lipids. On the basis of this polyphasic taxonomic data, strain MEBiC06243T should be classified as a novel species of the genus Neptunomonas proposed as Neptunomonas acidivorans sp. nov. The type strain is MEBiC06243T ( = KCCM 42975T = JCM 18291T). An emended description of the genus Neptunomonasis also given.
Published Online:
Funding
This study was supported by the:
Korea Institute of Ocean Science & Technology
(Award PE99212)
Marine BioResource Bank Program of the Ministry of Ocean & Fisheries, Korea
CollinsM. D.(1985). Isoprenoid quinone analysis in bacterial classification and identification. . In Chemical Methods in Bacterial Systematics, pp. 267–287. Edited by
GoodfellowM.,
MinnikinD. E.
. London:: Academic Press;.
DittmerJ. C. F.,
LesterR. L.(1964). A simple specific spray for the detection of phospholipids on thin-layer chromatograms. . J Lipid Res5, 126–127.[PubMed]
GiovannoniS. J.(1991). The polymerase chain reaction. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 177–203. Edited by
StackebrandtE.,
GoodfellowM.
. Chichester:: Wiley;.
HedlundB. P.,
GeiselbrechtA. D.,
BairT. J.,
StaleyJ. T.(1999). Polycyclic aromatic hydrocarbon degradation by a new marine bacterium, Neptunomonas naphthovorans gen. nov., sp. nov.. Appl Environ Microbiol65, 251–259.[PubMed]
JacinH.,
MishkinA. R.(1965). Separation of carbohydrates on borate-impregnated silica gel G plates. . J Chromatogr A18, 170–173. [View Article][PubMed]
KwonK. K.,
LeeH.-S.,
YangS. H.,
KimS.-J.(2005).Kordiimonas gwangyangensis gen. nov., sp. nov., a marine bacterium isolated from marine sediments that forms a distinct phyletic lineage (Kordiimonadales ord. nov.) in the ‘Alphaproteobacteria’. . Int J Syst Evol Microbiol55, 2033–2037. [View Article][PubMed]
LeeH.-W.,
ShinN.-R.,
LeeJ.,
RohS. W.,
WhonT. W.,
BaeJ.-W.(2012).Neptunomonas concharum sp. nov., isolated from a dead ark clam, and emended description of the genus Neptunomonas
. . Int J Syst Evol Microbiol62, 2657–2661. [View Article][PubMed]
LeeJ.-W.,
KwonK. K.,
AziziA.,
OhH.-M.,
KimW.,
BahkJ.-J.,
LeeD.-H.,
LeeJ.-H.(2013). Microbial community structures of methane hydrate bearing sediments in the Ulleung Basin, East Sea of Korea. . Mar Pet Geol47, 136–146. [View Article]
MinnikinD. E.,
O’DonnellA. G.,
GoodfellowM.,
AldersonG.,
AthalyeM.,
SchaalA.,
ParlettJ. H.(1984). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipid. . J Microbiol Methods2, 233–241. [View Article]
SmibertR. M.,
KriegN. R.(1994). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by
GerhardtP.,
MurrayR. G. E.,
WoodW. A.,
KriegN. R.
. Washington, DC:: American Society for Microbiology;.
StackebrandtE.,
LiesackW.(1993). Nucleic acids and classification. . In Handbook of New Bacterial Systematics, pp. 158–160. Edited by
GoodfellowM.,
O’DonnellA. G.
. London:: Academic Press;.
YangS.-H.,
SeoH.-S.,
OhH.-M.,
KimS.-J.,
LeeJ.-H.,
KwonK. K.(2013).Brumimicrobium mesophilum sp. nov., isolated from a tidal flat sediment, and emended descriptions of the genus Brumimicrobium and Brumimicrobium glaciale
. . Int J Syst Evol Microbiol63, 1105–1110. [View Article][PubMed]