Two strains of lactic acid bacteria, Nu27T and Nu29, were isolated from Nukadoko, rice bran paste for Japanese pickles. The isolates were Gram-stain-positive, rod-shaped, catalase-negative, non-motile and facultatively anaerobic lactic acid bacteria. The isolates showed identical 16S rRNA gene sequences. The closest relatives to strain Nu27T based on 16S rRNA gene sequence similarities were Lactobacillus versmoldensis KU-3T (98.9 % 16S rRNA gene sequence similarity), Lactobacillus nodensis iz4bT (96.3 %) and Lactobacillus tucceti CECT 5290T (97.2 %). DNA–DNA relatedness values revealed genotype separation of the two isolates from the above three species. Based on the physiological, biochemical and genotypic characteristics provided, the isolates represent a novel species of the genus Lactobacillus, for which name is Lactobacillus furfuricola proposed. The type strain is Nu 27T ( = JCM 18764T = NRIC 0900T = DSM 27174T).
Published Online:
Funding
This study was supported by the:
Japan Science and Technology Agency (JST)/Japan International Cooperation Agency (JICA)
Science and Technology Research Partnership for Sustainable Development (SATREPS)
EzakiT.,
HashimotoY.,
YabuuchiE.(1989). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol39, 224–229. [View Article]
GuindonS.,
GascuelO.(2003). A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. . Syst Biol52, 696–704. [View Article][PubMed]
KimuraM.(1980). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol16, 111–120. [View Article][PubMed]
KitaharaM.,
TakamineF.,
ImamuraT.,
BennoY.(2001).Clostridium hiranonis sp. nov., a human intestinal bacterium with bile acid 7α-dehydroxylating activity. . Int J Syst Evol Microbiol51, 39–44.[PubMed]
LarkinM. A.,
BlackshieldsG.,
BrownN. P.,
ChennaR.,
McGettiganP. A.,
McWilliamH.,
ValentinF.,
WallaceI. M.,
WilmA.& other authors (2007). Clustal W and Clustal X version 2.0. . Bioinformatics23, 2947–2948. [View Article][PubMed]
ManomeA.,
OkadaS.,
UchimuraT.,
KomagataK.(1998). The ratio of l-form to d-form of lactic acid as a criteria for the identification of lactic acid bacteria. . J Gen Appl Microbiol44, 371–374. [View Article][PubMed]
NaserS. M.,
ThompsonF. L.,
HosteB.,
GeversD.,
DawyndtP.,
VancanneytM.,
SwingsJ.(2005). Application of multilocus sequence analysis (MLSA) for rapid identification of Enterococcus species based on rpoA and pheS genes. . Microbiology151, 2141–2150. [View Article][PubMed]
OkadaS.,
HosoiY.,
TakahashiM.,
KozakiM.(1991). A rapid method for the determination of the fermentation types, homo- and hetero-fermentation, of lactic acid bacteria. . Bull JFCC7, 6–10. (in Japanese).
SakamotoM.,
OhkumaM.(2013).Bacteroides reticulotermitis sp. nov., isolated from the gut of a subterranean termite (Reticulitermes speratus). . Int J Syst Evol Microbiol63, 691–695. [View Article][PubMed]
SakamotoN.,
TanakaS.,
SonomotoK.,
NakayamaJ.(2011). 16S rRNA pyrosequencing-based investigation of the bacterial community in nukadoko, a pickling bed of fermented rice bran. . Int J Food Microbiol144, 352–359. [View Article][PubMed]
TamaokaJ.,
KomagataK.(1984). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. . FEMS Microbiol Lett25, 125–128. [View Article]