1887

Abstract

A strictly anaerobic bacterial strain, WK011, was isolated from a methanogenic reactor treating waste from cattle farms. The cells stained Gram-negative and were curved rods with a polar or subpolar flagellum. Spore formation was not observed. The optimum temperature for growth was 35 °C and the optimum pH was 6.7. Tests for oxidase, catalase and nitrate-reduction activities were negative. Hydrogen sulfide was produced. The strain fermented carbohydrates and produced acetate and propionate as major fermentation products. The genomic DNA G+C content was 41.7 mol%. The major cellular fatty acids were C, Cω9 and C dimethylacetal. The diagnostic diamino acid of the cell-wall peptidoglycan was -diaminopimelic acid. The most closely related species to strain WK011 on the basis of 16S rRNA gene sequences were and (95.6 % sequence similarity to the type strains of both species). It was shown by phylogenetic and phenotypic examination of the type strains of related species, including the second species of the genus , , that the two genera should be combined and that the two species of the genus should be transferred to the genus , as comb. nov. (type strain SH2 = ATCC 49691 = DSM 20765) and comb. nov. (type strain AA1 = ATCC 49689 = DSM 20756), with an emended description of the genus . Based on differences in the phylogenetic and phenotypic characteristics of strain WK011 from those of closely related species, the novel species sp. nov. is proposed to accommodate the strain. The type strain is WK011 ( = JCM 16475 = DSM 22929).

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2014-10-01
2019-11-19
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References

  1. Akasaka H., Ueki A., Hanada S., Kamagata Y., Ueki K.. ( 2003;). Propionicimonas paludicola gen. nov., sp. nov., a novel facultatively anaerobic, Gram-positive, propionate-producing bacterium isolated from plant residue in irrigated rice-field soil. . Int J Syst Evol Microbiol 53:, 1991–1998. [CrossRef][PubMed]
    [Google Scholar]
  2. Altschul S. F., Madden T. L., Schäffer A. A., Zhang J., Zhang Z., Miller W., Lipman D. J.. ( 1997;). Gapped blast and psi-blast: a new generation of protein database search programs. . Nucleic Acids Res 25:, 3389–3402. [CrossRef][PubMed]
    [Google Scholar]
  3. Blenden D. C., Goldberg H. S.. ( 1965;). Silver impregnation stain for Leptospira and flagella. . J Bacteriol 89:, 899–900.[PubMed]
    [Google Scholar]
  4. Collins M. D., Lawson P. A., Willems A., Cordoba J. J., Fernandez-Garayzabal J., Garcia P., Cai J., Hippe H., Farrow J. A. E.. ( 1994;). The phylogeny of the genus Clostridium: proposal of five new genera and eleven new species combinations. . Int J Syst Bacteriol 44:, 812–826. [CrossRef][PubMed]
    [Google Scholar]
  5. Felsenstein J..( 2006;). phylip (phylogeny inference package), version 3.66. Distributed by the author. . Department of Genome Sciences, University of Washington, Seattle, USA.
  6. Holdeman L. V., Cato E. P., Moore W. E. C.. ( 1977;). Anaerobe Laboratory Manual, , 4th edn.. Blacksburg, VA:: Virginia Polytechnic Institute and State University;.
    [Google Scholar]
  7. Komagata K., Suzuki K.. ( 1987;). Lipid and cell-wall analysis in bacterial systematics. . Methods Microbiol 19:, 161–207. [CrossRef]
    [Google Scholar]
  8. Marchandin H., Teyssier C., Campos J., Jean-Pierre H., Roger F., Gay B., Carlier J.-P., Jumas-Bilak E.. ( 2010;). Negativicoccus succinicivorans gen. nov., sp. nov., isolated from human clinical samples, emended description of the family Veillonellaceae and description of Negativicutes classis nov., Selenomonadales ord. nov. and Acidaminococcaceae fam. nov. in the bacterial phylum Firmicutes. . Int J Syst Evol Microbiol 60:, 1271–1279. [CrossRef][PubMed]
    [Google Scholar]
  9. Miller L. T.. ( 1982;). Single derivatization method for routine analysis of bacterial whole-cell fatty acid methyl esters, including hydroxy acids. . J Clin Microbiol 16:, 584–586.[PubMed]
    [Google Scholar]
  10. Miyagawa E., Azuma R., Suto E.. ( 1979;). Cellular fatty acid composition in Gram-negative obligately anaerobic rods. . J Gen Appl Microbiol 25:, 41–51. [CrossRef]
    [Google Scholar]
  11. Moore L. V. H., Bourne D. M., Moore W. E. C.. ( 1994;). Comparative distribution and taxonomic value of cellular fatty acids in thirty-three genera of anaerobic gram-negative bacilli. . Int J Syst Bacteriol 44:, 338–347. [CrossRef][PubMed]
    [Google Scholar]
  12. Nishiyama T., Ueki A., Kaku N., Ueki K.. ( 2009;). Clostridium sufflavum sp. nov., isolated from a methanogenic reactor treating cattle waste. . Int J Syst Evol Microbiol 59:, 981–986. [CrossRef][PubMed]
    [Google Scholar]
  13. Rainey F. A.. ( 2009;). Family X. Veillonellaceae Rogosa 1971b, 232AL. . In Bergey’s Manual of Systematic Bacteriology, , 2nd edn., vol. 3, p. 1059. Edited by De Vos P., Garrity G., Jones D., Krieg N. R., Ludwig W., Rainey F. A., Schleifer K.-H., Whitman W. B... New York:: Springer;.
    [Google Scholar]
  14. Rainey F. A., Hollen B. J., Small A.. ( 2009;). Genus I. Clostridium Prazmowski 1880, 23AL. . In Bergey’s Manual of Systematic Bacteriology, , 2nd edn., vol. 3, pp. 736–864. Edited by De Vos P., Garrity G., Jones D., Krieg N. R., Ludwig W., Rainey F. A., Schleifer K.-H., Whitman W. B... New York:: Springer;.
    [Google Scholar]
  15. Saitou N., Nei M.. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  16. Satoh A., Watanabe M., Ueki A., Ueki K.. ( 2002;). Physiological properties and phylogenetic affiliations of anaerobic bacteria isolated from roots of rice plants cultivated on a paddy field. . Anaerobe 8:, 233–246. [CrossRef]
    [Google Scholar]
  17. Schink B.. ( 2009;). Genus XVII. Propionispira Schink, Thompson and Zeikus 1983, 673VP. . In Bergey’s Manual of Systematic Bacteriology, , 2nd edn., vol. 3, pp. 1102–1103. Edited by De Vos P., Garrity G., Jones D., Krieg N. R., Ludwig W., Rainey F. A., Schleifer K.-H., Whitman W. B... New York:: Springer;.
    [Google Scholar]
  18. Schink B., Thompson T. E., Zeikus J. G.. ( 1982;). Characterization of Propionispira arboris gen. nov. sp. nov., a nitrogen-fixing anaerobe common to wetwoods of living trees. . J Gen Microbiol 128:, 2771–2779.
    [Google Scholar]
  19. Schleifer K.-H.. ( 2009;). Genus XXVI. Zymophilus Schleifer, Leuteritz, Weiss, Ludwig, Kirchhof and Seidel-Rufwer 1990, 26VP. . In Bergey’s Manual of Systematic Bacteriology, , 2nd edn., vol. 3, pp. 1119–1120. Edited by De Vos P., Garrity G., Jones D., Krieg N. R., Ludwig W., Rainey F. A., Schleifer K.-H., Whitman W. B... New York:: Springer;.
    [Google Scholar]
  20. Schleifer K. H., Leuteritz M., Weiss N., Ludwig W., Kirchhof G., Seidel-Rüfer H.. ( 1990;). Taxonomic study of anaerobic, gram-negative, rod-shaped bacteria from breweries: emended description of Pectinatus cerevisiiphilus and description of Pectinatus frisingensis sp. nov., Selenomonas lacticifex sp. nov., Zymophilus raffinosivorans gen. nov., sp. nov., and Zymophilus paucivorans sp. nov.. Int J Syst Bacteriol 40:, 19–27. [CrossRef][PubMed]
    [Google Scholar]
  21. Stackebrandt E., Pohla H., Kroppenstedt R., Hippe H., Woese C. R.. ( 1985;). 16S rRNA analysis of Sporomusa, Selenomonas, and Megasphaera: on the phylogenetic origin of Gram-positive Eubacteria. . Arch Microbiol 143:, 270–276. [CrossRef]
    [Google Scholar]
  22. Strömpl C., Tindall B. J., Jarvis G. N., Lünsdorf H., Moore E. R. B., Hippe H.. ( 1999;). A re-evaluation of the taxonomy of the genus Anaerovibrio, with the reclassification of Anaerovibrio glycerini as Anaerosinus glycerini gen. nov., comb. nov., and Anaerovibrio burkinabensis as Anaeroarcus burkinensis [corrig.] gen. nov., comb. nov.. Int J Syst Bacteriol 49:, 1861–1872. [CrossRef][PubMed]
    [Google Scholar]
  23. Sugawara Y., Ueki A., Abe K., Kaku N., Watanabe K., Ueki K.. ( 2011;). Propioniciclava tarda gen. nov., sp. nov., isolated from a methanogenic reactor treating waste from cattle farms. . Int J Syst Evol Microbiol 61:, 2298–2303. [CrossRef][PubMed]
    [Google Scholar]
  24. Thompson J. D., Higgins D. G., Gibson T. J.. ( 1994;). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. . Nucleic Acids Res 22:, 4673–4680. [CrossRef][PubMed]
    [Google Scholar]
  25. Tindall B. J.. ( 1990;). A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. . Syst Appl Microbiol 13:, 128–130. [CrossRef]
    [Google Scholar]
  26. Ueki A., Matsuda K., Ohtsuki C.. ( 1986;). Sulfate reduction in the anaerobic digestion of animal waste. . J Gen Appl Microbiol 32:, 111–123. [CrossRef]
    [Google Scholar]
  27. Ueki A., Abe K., Suzuki D., Kaku N., Watanabe K., Ueki K.. ( 2009;). Anaerosphaera aminiphila gen. nov., sp. nov., a glutamate-degrading, Gram-positive anaerobic coccus isolated from a methanogenic reactor treating cattle waste. . Int J Syst Evol Microbiol 59:, 3161–3167. [CrossRef][PubMed]
    [Google Scholar]
  28. Ueki A., Abe K., Ohtaki Y., Kaku N., Watanabe K., Ueki K.. ( 2011;). Bacteroides paurosaccharolyticus sp. nov., isolated from a methanogenic reactor treating waste from cattle farms. . Int J Syst Evol Microbiol 61:, 448–453. [CrossRef][PubMed]
    [Google Scholar]
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