1887

Abstract

Root-nodule bacteria were isolated from (Sw.) Willd. growing in the Cerrado Amazon region, State of Roraima, Brazil. The 16S rRNA gene sequences of six strains (BR 10250, BR 10248, BR 10249, BR 10251, BR 10252 and BR 10253) showed low similarities with currently described species of the genus . Phylogenetic analyses of sequences of five housekeeping genes (, , , and ) revealed EK05 to be the closest type strain (97.4 % sequence similarity or less). Chemotaxonomic data, including fatty acid profiles [with the major components C and summed feature 8 (Cω6/Cω7)], the slow growth rate and carbon compound utilization patterns supported the assignment of our strains to the genus . Results from DNA–DNA hybridizations and physiological traits differentiated our strains from the closest related species of the genus with validly published names. Sequences of symbiosis-related genes for nodulation () and nitrogen fixation () grouped together with those of EK05 and sp. strains BR 6610 (used as a commercial inoculant for in Brazil) and TUXTLAS-10 (previously observed in Central America). Based on these data, the six strains represent a novel species, for which the name sp. nov. is proposed. The type strain is BR 10250 ( = HAMBI 3600).

Funding
This study was supported by the:
  • CNPq
  • Embrapa
  • Murdoch University
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2014-10-01
2024-04-25
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